Hong Kong Baptist University

About: Hong Kong Baptist University is a education organization based out in Hong Kong, China. It is known for research contribution in the topics: Population & China. The organization has 7811 authors who have published 18919 publications receiving 555274 citations. The organization is also known as: Hong Kong Baptist College & HKBU.

Conjugating a groove-binding motif to an Ir(III) complex for the enhancement of G-quadruplex probe behavior

Abstract: In this study, the reported G-quadruplex groove binder benzo[d,e]isoquinoline was linked to a cyclometallated Ir(iii) complex to generate a highly selective DNA probe 1 that retains the favorable photophysical properties of the parent complex. The linked complex 1 showed advantages of both parent complex 2 and groove binder 3. Similar to 3, the conjugated complex 1 exhibits a superior affinity and selectivity for G-quadruplex DNA over other conformations of DNA or proteins, with the fold enhancement ratio obviously improved compared with parent complex 2. The molecular modelling revealed a groove-binding mode between complex 1 and G-quadruplex. Meanwhile 1 also possesses the prominent advantages of transition metal complex probes such as a large Stokes shift and long lifetime phosphorescence, which could be recognized in strong fluorescence media through time-resolved emission spectroscopy (TRES). We then employed 1 to develop a detection assay for AGR2, a potential cancer biomarker, as a "proof-of-principle" demonstration of the application of a linked complex for DNA-based detection in diluted fetal bovine serum. We anticipate that this conjugation method may be further employed in the development of DNA probes and have applications in label-free DNA-based diagnostic platforms.

Phase synchronization in coupled nonidentical excitable systems and array-enhanced coherence resonance

Abstract: We study the dynamics of a lattice of coupled nonidentical Fitz Hugh-Nagumo system subject to independent external noise. It is shown that these stochastic oscillators can lead to global synchronization behavior without an external signal. With the increase of the noise intensity, the system exhibits coherence resonance behavior. Coupling can enhance greatly the noise-induced coherence in the system.

Dynamic Label Graph Matching for Unsupervised Video Re-Identification

Abstract: Label estimation is an important component in an unsupervised person re-identification (re-ID) system. This paper focuses on cross-camera label estimation, which can be subsequently used in feature learning to learn robust re-ID models. Specifically, we propose to construct a graph for samples in each camera, and then graph matching scheme is introduced for cross-camera labeling association. While labels directly output from existing graph matching methods may be noisy and inaccurate due to significant cross-camera variations, this paper proposes a dynamic graph matching (DGM) method. DGM iteratively updates the image graph and the label estimation process by learning a better feature space with intermediate estimated labels. DGM is advantageous in two aspects: 1) the accuracy of estimated labels is improved significantly with the iterations; 2) DGM is robust to noisy initial training data. Extensive experiments conducted on three benchmarks including the large-scale MARS dataset show that DGM yields competitive performance to fully supervised baselines, and outperforms competing unsupervised learning methods.

Ascorbic acid and reactive oxygen species are involved in the inhibition of seed germination by abscisic acid in rice seeds

Abstract: The antagonism between abscisic acid (ABA) and gibberellin (GA) plays a key role in controlling seed germination, but the mechanism of antagonism during this process is not known. The possible links among ABA, reactive oxygen species (ROS), ascorbic acid (ASC), and GA during rice seed germination were investigated. Unlike in non-seed tissues where ROS production is increased by ABA, ABA reduced ROS production in imbibed rice seeds, especially in the embryo region. Such reduced ROS also led to an inhibition of ASC production. GA accumulation was also suppressed by a reduced ROS and ASC level, which was indicated by the inhibited expression of GA biosynthesis genes, amylase genes, and enzyme activity. Application of exogenous ASC can partially rescue seed germination from ABA treatment. Production of ASC, which acts as a substrate in GA biosynthesis, was significantly inhibited by lycorine which thus suppressed the accumulation of GA. Consequently, expression of GA biosynthesis genes was suppressed by the low levels of ROS and ASC in ABA-treated seeds. It can be concluded that ABA regulates seed germination in multiple dimensions. ROS and ASC are involved in its inhibition of GA biosynthesis.

Small interfering RNA specific for N-methyl-D-aspartate receptor 2B offers neuroprotection to dopamine neurons through activation of MAP kinase.

Abstract: In the present study, N-methyl-D-aspartate receptor 2B (NR2B)-specific siRNA was applied in parkinsonian models. Our previous results showed that reduction in expression of N-methyl-D-aspartate receptor 1 (NR1), the key subunit of N-methyl-D-aspartate receptors, by antisense oligos amelio-rated the motor symptoms in the 6-hydroxydopamine (6-OHDA)-lesioned rat, an animal model of Parkinson's disease (PD) [Lai et al.: Neurochem Int 2004;45:11-22]. To further the investigation on the efficacy of gene silencing, small interference RNA (siRNA) specific for the NR2B subunit was designed and administered in the striatum of 6-OHDA-lesioned rats. The present results show that administration of NR2B-specific siRNA decreased the number of apomorphine-induced rotations in the lesioned rats and that there was a significant reduction in NR2B proteins levels after NR2B-specific siRNA administration. Furthermore, attenuation of the loss of dopaminergic neurons was found in both the striatal and substantia nigra regions of the 6-OHDA-lesioned rats that had been continuously infused with siRNA for 7 days. In addition, a significant upregulation of p-p44/42 MAPK (ERK1/2; Thr202/Tyr204) and p-CREB (Ser133) in striatal neurons was found. These results suggest that application of the gene silencing targeting NR2B could be a potential treatment of PD, and they also revealed the possibility of NR2B-specific siRNA being involved in the prosurvival pathway.