Hong Kong Polytechnic University

About: Hong Kong Polytechnic University is a education organization based out in Hong Kong, China. It is known for research contribution in the topics: Computer science & Tourism. The organization has 29633 authors who have published 72136 publications receiving 1956312 citations. The organization is also known as: HKPU & PolyU.

Blockchain technology in supply chain operations: Applications, challenges and research opportunities

Abstract: Blockchain is a technology with unique combination of features such as decentralized structure, distributed notes and storage mechanism, consensus algorithm, smart contracting, and asymmetric encryption to ensure network security, transparency and visibility. Blockchain has immense potential to transform supply chain (SC) functions, from SC provenance, business process reengineering to security enhancement. More and more studies exploring the use of blockchain in SCs have appeared in recent years. In this paper, we consider a total of 178 articles and examine all the relevant research done in the field associated with the use of blockchain integration in SC operations. We highlight the corresponding opportunities, possible societal impacts, current state-of-the-art technologies along with major trends and challenges. We examine several industrial sectors such as shipping, manufacturing, automotive, aviation, finance, technology, energy, healthcare, agriculture and food, e-commerce, and education among others that can be successfully revamped with blockchain based technologies through enhanced visibility and business process management. A future research agenda is established which lays the solid foundation for further studies on this important emerging research area.

Sensitive and Inexpensive Molecular Test for Falciparum Malaria: Detecting Plasmodium falciparum DNA Directly from Heat-Treated Blood by Loop-Mediated Isothermal Amplification,

Abstract: Background: Malaria is one of the most important parasitic infections in humans. A sensitive diagnostic test for malaria that could be applied at the community level could be useful in programs to control the disease. The aim of the present work was to develop a simple, inexpensive molecular test for Plasmodium falciparum . Methods: Blood was collected from controls (n = 100) and from patients diagnosed with falciparum malaria infection (n = 102), who were recruited to the study. Heat-treated blood samples were tested by a loop-mediated isothermal amplification (LAMP) assay for P. falciparum . Results were interpreted by a turbidity meter in real time or visually at the end of the assay. To evaluate the assay, DNA from these samples was purified and tested by PCR. Results from the LAMP and PCR assays were compared. Results: The LAMP assay detected P. falciparum directly from heat-treated blood. The quantitative data from the assay correlated to the parasite counts obtained by blood-film microscopic analyses. When we used the PCR assay as the comparison method, the sensitivity and specificity of the LAMP assay were 95% and 99%, respectively. Conclusions: Unlike PCR, the LAMP assay does not require purified DNA for efficient DNA amplification, thereby reducing the cost and turnaround time for P. falciparum diagnosis. The assay requires only basic instruments, and assay positivity can be verified by visual inspection.