Indian Agricultural Statistics Research Institute

About: Indian Agricultural Statistics Research Institute is a facility organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Small area estimation. The organization has 454 authors who have published 870 publications receiving 7987 citations.

Genome wide association mapping of agro-morphological traits among a diverse collection of finger millet (Eleusine coracana L.) genotypes using SNP markers.

Abstract: Finger millet (Eleusine coracana L.) is an important dry-land cereal in Asia and Africa because of its ability to provide assured harvest under extreme dry conditions and excellent nutritional properties. However, the genetic improvement of the crop is lacking in the absence of suitable genomic resources for reliable genotype-phenotype associations. Keeping this in view, a diverse global finger millet germplasm collection of 113 accessions was evaluated for 14 agro-morphological characters in two environments viz. ICAR-Vivekananda Institute of Hill Agriculture, Almora (E1) and Crop Research Centre (CRC), GBPUA&T, Pantnagar (E2), India. Principal component analysis and cluster analysis of phenotypic data separated the Indian and exotic accessions into two separate groups. Previously generated SNPs through genotyping by sequencing (GBS) were used for association mapping to identify reliable marker(s) linked to grain yield and its component traits. The marker trait associations were determined using single locus single trait (SLST), multi-locus mixed model (MLMM) and multi-trait mixed model (MTMM) approaches. SLST led to the identification of 20 marker-trait associations (MTAs) (p value<0.01 and <0.001) for 5 traits. While advanced models, MLMM and MTMM resulted in additional 36 and 53 MTAs, respectively. Nine MTAs were common out of total 109 associations in all the three mapping approaches (SLST, MLMM and MTMM). Among these nine SNPs, five SNP sequences showed homology to candidate genes of Oryza sativa (Rice) and Setaria italica (Foxtail millet), which play an important role in flowering, maturity and grain yield. In addition, 67 and 14 epistatic interactions were identified for 10 and 7 traits at E1 and E2 locations, respectively. Hence, the 109 novel SNPs associated with important agro-morphological traits, reported for the first time in this study could be precisely utilized in finger millet genetic improvement after validation.

Exploration of new drug-like inhibitors for serine/threonine protein phosphatase 5 of Plasmodium falciparum: a docking and simulation study.

Abstract: Protein phosphorylation is an important mechanism that implicates in physiology of any organism including parasitic protozoa. Metallic protein Ser/Thr protein phosphatase 5 (PP5) controls various cellular signaling pathways of Plasmodium falciparum. The structure and inhibitory mechanism of PP5 in P. falciparum is not known. In fact, no experimental structural data are available for P. falciparum Ser/Thr protein phosphatase 5 (PfPP5) till date. Hence, we have proposed computer-generated model of catalytic subunit of PfPP5 and its inhibitory mechanism was analyzed. A set of 42 known natural inhibitors of protein phosphate family were docked against metal-binding catalytic site of PfPP5 and we found that cantharidin and its derivatives shows better binding energy among them. Similarity search was performed by taking these compounds as lead compounds against PubChem and ChemBank. The search result provides 3703 similar compounds; out of which 2245 qualified the Lipinski rule of five. Further, virtual screening of these compounds was performed and selected top 25 were selected on the basis of binding energy. In continuation, rigid and flexible docking of these screened compounds was performed to get the insight of interactions. Finally, top 5 compounds were verified for ADMET properties, and then, all are subjected to MD simulations for 25 ns in order to validate their stability. Compounds CBI: 3554182, CID: 23561913, and CID: 21168680 showed most stable binding, although some of hydrogen bonds pairing varied throughout simulation. These finding would be helpful to the medicinal chemists for the development of antimalarial drugs to combat this deadly disease.

Geostatistical and fuzzy clustering approach for delineation of site-specific management zones and yield-limiting factors in irrigated hot arid environment of India

Abstract: Delineation of management zones (MZs) are needed to manage fields in order to maximize economic return, minimize environmental impact, and improve soil and crop management. The MZs of uniform production potential may offer an effective solution to nutrient management. In this study, a total of 122 geo-referenced representative surface (0–250 mm depth) soil samples were collected from the study area covering an area of 6296 ha. Soil samples were analysed for pH, EC, CaCO3, organic carbon (SOC), available nitrogen (AN), available phosphorus (AP), available potassium (AK) and micronutrients (Fe, Mn, Zn and Cu). Their spatial variability was analyzed and spatial distribution maps were constructed using geostatistical techniques. Geostatistical analysis showed that exponential, rational quadratic, tetraspherical, pentaspherical and circular models were the best-fit models for soil properties and available nutrients. Further, geographical weighted principal component analysis (GWPCA) and possibilistic fuzzy C-means (PFCM) clustering algorithm were carried out to delineate the management zones based on optimum clusters identified using fuzzy performance index (FPI) and normalized classification entropy (NCE). The results revealed that the optimum number of MZs for this study area was four and there was heterogeneity in soil nutrients in four MZs. The study indicated that MZ-based soil test crop response recommendation reduces the application quantity of fertilizer significantly at a large extent. Therefore, the management zone concept can reduce agricultural inputs and environmental pollution, and maximize crop production.