Institution
Indian Institute of Pulses Research
Facility•Kanpur, India•
About: Indian Institute of Pulses Research is a facility organization based out in Kanpur, India. It is known for research contribution in the topics: Population & Genetic diversity. The organization has 396 authors who have published 646 publications receiving 8174 citations.
Topics: Population, Genetic diversity, Agriculture, Molecular breeding, Gene
Papers published on a yearly basis
Papers
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TL;DR: The current review aims to offer a deeper understanding of TFs engaged in regulating plant’s response under drought stress and to devise potential strategies to improve plant tolerance against drought.
Abstract: Increasing vulnerability of plants to a variety of stresses such as drought, salt and extreme temperatures poses a global threat to sustained growth and productivity of major crops. Of these stresses, drought represents a considerable threat to plant growth and development. In view of this, developing staple food cultivars with improved drought tolerance emerges as the most sustainable solution towards improving crop productivity in a scenario of climate change. In parallel, unraveling the genetic architecture and the targeted identification of molecular networks using modern “OMICS” analyses, that can underpin drought tolerance mechanisms, is urgently required. Importantly, integrated studies intending to elucidate complex mechanisms can bridge the gap existing in our current knowledge about drought stress tolerance in plants. It is now well established that drought tolerance is regulated by several genes, including transcription factors (TFs) that enable plants to withstand unfavorable conditions, and these remain potential genomic candidates for their wide application in crop breeding. These TFs represent the key molecular switches orchestrating the regulation of plant developmental processes in response to a variety of stresses. The current review aims to offer a deeper understanding of TFs engaged in regulating plant’s response under drought stress and to devise potential strategies to improve plant tolerance against drought.
499 citations
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International Crops Research Institute for the Semi-Arid Tropics1, University of Florida2, Hokkaido University3, Leibniz Association4, Indian Institute of Pulses Research5, Indian Agricultural Research Institute6, Central Arid Zone Research Institute7, University of Agricultural Sciences, Bangalore8
TL;DR: Nine QTL clusters containing QTLs for several drought tolerance traits have been identified that can be targeted for molecular breeding and one cluster present on CaLG04 has a high potential to enhance drought tolerance in chickpea improvement.
Abstract: Key message
Analysis of phenotypic data for 20 drought tolerance traits in 1–7 seasons at 1–5 locations together with genetic mapping data for two mapping populations provided 9 QTL clusters of which one present on CaLG04 has a high potential to enhance drought tolerance in chickpea improvement.
293 citations
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International Crops Research Institute for the Semi-Arid Tropics1, United States Department of Agriculture2, Indian Institute of Pulses Research3, Egerton University4, Agricultural Research Organization, Volcani Center5, Indian Agricultural Research Institute6, Jawaharlal Nehru Agricultural University7, Mahatma Phule Krishi Vidyapeeth8, University of Agricultural Sciences, Dharwad9, Crops Research Institute10
TL;DR: The use of integrated genomics and breeding approach in these legume crops to enhance crop productivity in marginal environments ensuring food security in developing countries is proposed.
278 citations
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Kalyani Government Engineering College1, Indian Agricultural Research Institute2, Indian Institute of Pulses Research3, Institute of Agricultural Sciences, Banaras Hindu University4, University of Agricultural Sciences, Dharwad5, Dr. Panjabrao Deshmukh Krishi Vidyapeeth6, International Crops Research Institute for the Semi-Arid Tropics7, University of California, Davis8
TL;DR: A comprehensive set of validated genic simple sequence repeat (SSR) markers was developed as an important genomic resource for diversity analysis and genetic mapping in pigeonpea and was validated for expected allelic segregation in the reference mapping population.
Abstract: Pigeonpea [Cajanus cajan (L.) Millspaugh], one of the most important food legumes of semi-arid tropical and subtropical regions, has limited genomic resources, particularly expressed sequence based (genic) markers. We report a comprehensive set of validated genic simple sequence repeat (SSR) markers using deep transcriptome sequencing, and its application in genetic diversity analysis and mapping. In this study, 43,324 transcriptome shotgun assembly unigene contigs were assembled from 1.696 million 454 GS-FLX sequence reads of separate pooled cDNA libraries prepared from leaf, root, stem and immature seed of two pigeonpea varieties, Asha and UPAS 120. A total of 3,771 genic-SSR loci, excluding homopolymeric and compound repeats, were identified; of which 2,877 PCR primer pairs were designed for marker development. Dinucleotide was the most common repeat motif with a frequency of 60.41%, followed by tri- (34.52%), hexa- (2.62%), tetra- (1.67%) and pentanucleotide (0.76%) repeat motifs. Primers were synthesized and tested for 772 of these loci with repeat lengths of ≥18 bp. Of these, 550 markers were validated for consistent amplification in eight diverse pigeonpea varieties; 71 were found to be polymorphic on agarose gel electrophoresis. Genetic diversity analysis was done on 22 pigeonpea varieties and eight wild species using 20 highly polymorphic genic-SSR markers. The number of alleles at these loci ranged from 4-10 and the polymorphism information content values ranged from 0.46 to 0.72. Neighbor-joining dendrogram showed distinct separation of the different groups of pigeonpea cultivars and wild species. Deep transcriptome sequencing of the two parental lines helped in silico identification of polymorphic genic-SSR loci to facilitate the rapid development of an intra-species reference genetic map, a subset of which was validated for expected allelic segregation in the reference mapping population. We developed 550 validated genic-SSR markers in pigeonpea using deep transcriptome sequencing. From these, 20 highly polymorphic markers were used to evaluate the genetic relationship among species of the genus Cajanus. A comprehensive set of genic-SSR markers was developed as an important genomic resource for diversity analysis and genetic mapping in pigeonpea.
251 citations
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International Crops Research Institute for the Semi-Arid Tropics1, Indian Institute of Pulses Research2, Ethiopian Institute of Agricultural Research3, Egerton University4, Guru Gobind Singh Indraprastha University5, University of Agricultural Sciences, Bangalore6, Hokkaido University7, United Nations University8
TL;DR: This study provides significant MTAs for drought andHeat tolerance in chickpea that can be used, after validation, in molecular breeding for developing superior varieties with enhanced drought and heat tolerance.
Abstract: To understand the genetic basis of tolerance to drought and heat stresses in chickpea, a comprehensive association mapping approach has been undertaken Phenotypic data were generated on the reference set (300 accessions, including 211 mini-core collection accessions) for drought tolerance related root traits, heat tolerance, yield and yield component traits from 1–7 seasons and 1–3 locations in India (Patancheru, Kanpur, Bangalore) and three locations in Africa (Nairobi, Egerton in Kenya and Debre Zeit in Ethiopia) Diversity Array Technology (DArT) markers equally distributed across chickpea genome were used to determine population structure and three sub-populations were identified using admixture model in STRUCTURE The pairwise linkage disequilibrium (LD) estimated using the squared-allele frequency correlations (r2; when r2<020) was found to decay rapidly with the genetic distance of 5 cM For establishing marker-trait associations (MTAs), both genome-wide and candidate gene-sequencing based association mapping approaches were conducted using 1,872 markers (1,072 DArTs, 651 single nucleotide polymorphisms [SNPs], 113 gene-based SNPs and 36 simple sequence repeats [SSRs]) and phenotyping data mentioned above employing mixed linear model (MLM) analysis with optimum compression with P3D method and kinship matrix As a result, 312 significant MTAs were identified and a maximum number of MTAs (70) was identified for 100-seed weight A total of 18 SNPs from 5 genes (ERECTA, 11 SNPs; ASR, 4 SNPs; DREB, 1 SNP; CAP2 promoter, 1 SNP and AMDH, 1SNP) were significantly associated with different traits This study provides significant MTAs for drought and heat tolerance in chickpea that can be used, after validation, in molecular breeding for developing superior varieties with enhanced drought and heat tolerance
249 citations
Authors
Showing all 403 results
Name | H-index | Papers | Citations |
---|---|---|---|
Narinder Singh | 45 | 452 | 9028 |
Narendra Singh | 36 | 289 | 9187 |
Sanjeev K. Gupta | 28 | 280 | 2773 |
Abhishek Bohra | 27 | 98 | 2906 |
Shiv Kumar | 25 | 116 | 1758 |
Sushil K. Chaturvedi | 24 | 52 | 1866 |
Aamir W. Khan | 24 | 38 | 2000 |
Jitendra Kumar | 23 | 86 | 1741 |
Kusal K Das | 20 | 110 | 1759 |
Aditya Pratap | 19 | 86 | 1415 |
A. N. Ganeshamurthy | 18 | 59 | 922 |
N. Nadarajan | 17 | 34 | 1912 |
Subhojit Datta | 17 | 60 | 1573 |
Sujay Rakshit | 17 | 90 | 1105 |
A. K. Choudhary | 16 | 39 | 823 |