Indian Institute of Technology Guwahati

About: Indian Institute of Technology Guwahati is a education organization based out in Guwahati, Assam, India. It is known for research contribution in the topics: Adsorption & Catalysis. The organization has 6933 authors who have published 17102 publications receiving 257351 citations.

Tissue Engineered Skin and Wound Healing: Current Strategies and Future Directions

Abstract: The global volume of skin damage or injuries has major healthcare implications and, accounts for about half of the world's annual expenditure in the healthcare sector. In the last two decades, tissue-engineered skin constructs have shown great promise in the treatment of various skin-related disorders such as deep burns and wounds. The treatment methods for skin replacement and repair have evolved from utilization of autologous epidermal sheets to more complex bilayered cutaneous tissue engineered skin substitutes. However, inadequate vascularization, lack of flexibility in drug/growth factors loading and inability to reconstitute skin appendages such as hair follicles limits their utilization for restoration of normal skin anatomy on a routine basis. Recent advancements in cutting-edge technology from stem cell biology, nanotechnology, and various vascularization strategies have provided a tremendous springboard for researchers in developing and manipulating tissue engineered skin substitutes for improved skin regeneration and wound healing. This review summarizes the overview of skin tissue engineering and wound healing. Herein, developments and challenges of various available biomaterials, cell sources and in vitro skin models (full thickness and wound healing models) in tissue-engineered skin research are discussed. Furthermore, central to the discussion is the inclusion of various innovative strategies starting from stem cells, nanotechnology, vascularization strategies, microfluidics to three dimensional (3D) bioprinting based strategies for generation of complex skin mimics. The review then moves on to highlight the future prospects of advanced construction strategies of these bioengineered skin constructs and their contribution to wound healing and skin regeneration on current practice.

Speaker localization using excitation source information in speech

Abstract: This paper presents the results of simulation and real room studies for localization of a moving speaker using information about the excitation source of speech production. The first step in localization is the estimation of time-delay from speech collected by a pair of microphones. Methods for time-delay estimation generally use spectral features that correspond mostly to the shape of vocal tract during speech production. Spectral features are affected by degradations due to noise and reverberation. This paper proposes a method for localizing a speaker using features that arise from the excitation source during speech production. Experiments were conducted by simulating different noise and reverberation conditions to compare the performance of the time-delay estimation and source localization using the proposed method with the results obtained using the spectrum-based generalized cross correlation (GCC) methods. The results show that the proposed method shows lower number of discrepancies in the estimated time-delays. The bias, variance and the root mean square error (RMSE) of the proposed method is consistently equal or less than the GCC methods. The location of a moving speaker estimated using the time-delays obtained by the proposed method are closer to the actual values, than those obtained by the GCC method.

Effect of Functional Groups of Self-Assembled Monolayers on Protein Adsorption and Initial Cell Adhesion.

Abstract: Surface modification plays a vital role in regulating protein adsorption and subsequently cell adhesion. In the present work, we prepared nanoscaled modified surfaces using silanization and characterized them using Fourier-transform infrared spectroscopy (FTIR), water contact angle (WCA), and atomic force microscopy (AFM). Five different (amine, octyl, mixed, hybrid, and COOH) surfaces were prepared based on their functionality and varying wettability and their effect on protein adsorption and initial cell adhesion was investigated. AFM analysis revealed nanoscale roughness on all modified surfaces. Fetal bovine serum (FBS) was used for protein adsorption experiment and effect of FBS was analyzed on initial cell adhesion kinetics (up to 6 h) under three different experimental conditions: (a) with FBS in media, (b) with preadsorbed FBS on surfaces, and (c) incomplete media, i.e., without FBS. Various cell features such as cell morphology/circularity, cell area and nuclei size were also studied for the abov...