Indiana University

About: Indiana University is a education organization based out in Bloomington, Indiana, United States. It is known for research contribution in the topics: Population & Poison control. The organization has 64480 authors who have published 150058 publications receiving 6392902 citations. The organization is also known as: Indiana University system & indiana.edu.

Phylogenetic Stains: Ribosomal RNA-Based Probes for the Identification of Single Cells

Abstract: Rapid phylogenetic identification of single microbial cells was achieved with a new staining method. Formaldehyde-fixed, intact cells were hybridized with fluorescently labeled oligodeoxynucleotides complementary to 16S ribosomal RNA (rRNA) and viewed by fluorescence microscopy. Because of the abundance of rRNA in cells, the binding of the fluorescent probes to individual cells is readily visualized. Phylogenetic identification is achieved by the use of oligonucleotides (length 17 to 34 nucleotides) that are complementary to phylogenetic group-specific 16S rRNA sequences. Appropriate probes can be composed of oligonucleotide sequences that distinguish between the primary kingdoms (eukaryotes, eubacteria, archaebacteria) and between closely related organisms. The simultaneous use of multiple probes, labeled with different fluorescent dyes, allows the identification of different cell types in the same microscopic field. Quantitative microfluorimetry shows that the amount of an rRNA-specific probe that binds to Escherichia coli varies with the ribosome content and therefore reflects growth rate.

Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4

Abstract: We conducted a combined genome-wide association study (GWAS) of 7,481 individuals with bipolar disorder (cases) and 9,250 controls as part of the Psychiatric GWAS Consortium. Our replication study tested 34 SNPs in 4,496 independent cases with bipolar disorder and 42,422 independent controls and found that 18 of 34 SNPs had P < 0.05, with 31 of 34 SNPs having signals with the same direction of effect (P = 3.8 × 10−7). An analysis of all 11,974 bipolar disorder cases and 51,792 controls confirmed genome-wide significant evidence of association for CACNA1C and identified a new intronic variant in ODZ4. We identified a pathway comprised of subunits of calcium channels enriched in bipolar disorder association intervals. Finally, a combined GWAS analysis of schizophrenia and bipolar disorder yielded strong association evidence for SNPs in CACNA1C and in the region of NEK4-ITIH1-ITIH3-ITIH4. Our replication results imply that increasing sample sizes in bipolar disorder will confirm many additional loci.

Epistemological and methodological bases of naturalistic inquiry.

Abstract: How suitable is the rationalistic paradigm for research focusing on human behavior? Proposing that naturalistic inquiry better serves the social/ behavioral sciences, the authors define the differences between the two paradigms and suggest criteria for ensuring the trustworthiness of naturalistic inquiry.

Lack of effect of induction of hypothermia after acute brain injury.

Abstract: Background Induction of hypothermia in patients with brain injury was shown to improve outcomes in small clinical studies, but the results were not definitive. To study this issue, we conducted a multicenter trial comparing the effects of hypothermia with those of normothermia in patients with acute brain injury. Methods The study subjects were 392 patients 16 to 65 years of age with coma after sustaining closed head injuries who were randomly assigned to be treated with hypothermia (body temperature, 33°C), which was initiated within 6 hours after injury and maintained for 48 hours by means of surface cooling, or normothermia. All patients otherwise received standard treatment. The primary outcome measure was functional status six months after the injury. Results The mean age of the patients and the type and severity of injury in the two treatment groups were similar. The mean (±SD) time from injury to randomization was 4.3±1.1 hours in the hypothermia group and 4.1±1.2 hours in the normothermia group, a...

Visualizing knowledge domains

Abstract: L'A. passe en revue les techniques de visualisation utilisees pour representer de facon cartographique la structure de domaine des disciplines scientifiques, et pour soutenir la recherche d'information et la classification. Un bref historique montre que la visualisation des domaines de connaissances s'enracine dans des disciplines telles que la scientometrie, la bibliometrie et l'analyse de citations, ainsi que la visualisation scientifique. L'A. analyse les principales etapes du processus de visualisation des domaines de connaissances : unites d'analyse, mesures, similarites entre unites. Differentes techniques couramment utilisees pour l'analyse et la visualisation des connaissances sont passees en revue : techniques de reduction de la dimensionnalite, analyse par clusters, configuration spatiale, visualisation et conception d'interaction. Differentes approches sont appliquees pour engendrer et comparer diverses representations cartographiques de la recherche sur la visualisation des domaines de connaissances. Ces cartes mettent en valeur les relations entre l'analyse de citations, la bibliometrie, la semantique et la visualisation de l'information. Augmenter l'accessibilite de la visualisation des domaines aupres des non-experts, appliquer la visualisation des domaines de connaissances pour mieux repondre a des questions pragmatiques, favoriser la collaboration et la diffusion des resultats entre chercheurs, developper des algorithmes plus robustes, comptent parmi les directions de recherche les plus prometteuses.