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Showing papers by "Institute of Chartered Accountants of Nigeria published in 2012"


Journal ArticleDOI
TL;DR: The aim was to build an algorithm along with a scoring system for histopathologic classification of liver lesions that covers the entire spectrum of lesions in morbidly obese patients, and suggest describing liver lesions using the SAF score.

645 citations


Journal ArticleDOI
TL;DR: The critical role of the Na(v)1.5 N-terminal region in channel function and the dominant-negative effect of trafficking-defective channels occurring through α-subunit interaction are reported for the first time.
Abstract: Patch-clamp recordings in HEK293 cells demonstrated that R104W, R121W, and DNter abolished the sodium current INa. Moreover, R104W and R121W mutations exerted a strong dominant-negative effect on wild-type (WT) channels. Immunocytochemistry of rat neonatal cardiomyocytes revealed that both mutants were mostly retained in the endoplasmic reticulum and that their co-expression with WT channels led to WT channel retention. Furthermore, co-immunoprecipitation experiments showed that Nav1.5-subunits were interacting with each other, even when mutated, deciphering the mutation dominant-negative effect. Both mutants were mostly degraded by the ubiquitin-proteasome system, while DNter was addressed to the membrane, and Nter expression induced a two-fold increase in INa. In addition, the co-expression of N-terminal mutants with the gating-defective but traffick- ing-competent R878C-Nav1.5 mutant gave rise to a small INa. Conclusion This study reports for the first time the critical role of the Nav1.5 N-terminal region in channel function and the dominant-negative effect of trafficking-defective channels occurring through a-subunit interaction. Arrhythmia † Brugada syndrome † Nav1.5 † SCN5A † Sodium

92 citations


Journal ArticleDOI
TL;DR: Data show distinct gene signature and macrophage abundance in the two compartments of SAT, with dSAT more closely related to VAT than to sSAT in terms of inflammation and relation with the severity of liver diseases in morbid obesity.

88 citations


Journal ArticleDOI
31 Aug 2012-PLOS ONE
TL;DR: The results strongly suggest that the regulation of PAI-1 molecule could be under the influence of several miRNAs whose measurement in the plasma of patients could be envisaged as a biomarker for inflammatory and thrombotic disorders.
Abstract: In this work, we assessed whether SERPINE1 expression could be under the influence of microRNAs (miRNAs) predicted to bind the SERPINE1 3′UTR region. We specifically focused on the 3′UTR region harboring a common polymorphism, rs1050955, that have been found associated to SERPINE1 monocyte expression, and investigated whether the presence of different alleles at rs1050955 could modify the miRNAs binding efficiency and affect PAI-1 protein levels. We demonstrated that, in human umbilical vein endothelial cells, both miR-421 and miR-30c directly interacted with PAI-1 mRNA to inhibit the expression of the associated protein. However, these inhibitory mechanisms were independent on the allele present at the rs1050955 locus. We further showed that miR-421 levels correlated with PAI-1 activity in the plasma sample of 40 patients with venous thrombosis. Our results strongly suggest that the regulation of PAI-1 molecule could be under the influence of several miRNAs whose measurement in the plasma of patients could be envisaged as a biomarker for inflammatory and thrombotic disorders.

61 citations


Journal ArticleDOI
TL;DR: Analysis of expression of 271 transcripts helps understanding the relative importance of environmental and individual factors that control the expression of human AT genes and therefore may foster strategies aimed at improving AT function in metabolic diseases.
Abstract: Weight control diets favorably affect parameters of the metabolic syndrome and delay the onset of diabetic complications. The adaptations occurring in adipose tissue (AT) are likely to have a profound impact on the whole body response as AT is a key target of dietary intervention. Identification of environmental and individual factors controlling AT adaptation is therefore essential. Here, expression of 271 transcripts, selected for regulation according to obesity and weight changes, was determined in 515 individuals before, after 8-week low-calorie diet-induced weight loss, and after 26-week ad libitum weight maintenance diets. For 175 genes, opposite regulation was observed during calorie restriction and weight maintenance phases, independently of variations in body weight. Metabolism and immunity genes showed inverse profiles. During the dietary intervention, network-based analyses revealed strong interconnection between expression of genes involved in de novo lipogenesis and components of the metabolic syndrome. Sex had a marked influence on AT expression of 88 transcripts, which persisted during the entire dietary intervention and after control for fat mass. In women, the influence of body mass index on expression of a subset of genes persisted during the dietary intervention. Twenty-two genes revealed a metabolic syndrome signature common to men and women. Genetic control of AT gene expression by cis signals was observed for 46 genes. Dietary intervention, sex, and cis genetic variants independently controlled AT gene expression. These analyses help understanding the relative importance of environmental and individual factors that control the expression of human AT genes and therefore may foster strategies aimed at improving AT function in metabolic diseases.

60 citations


Journal ArticleDOI
TL;DR: It is demonstrated that rapid removal of circulating lipoprotein particles by LDL apheresis transitorily reduces RCT and is without impact on the intrinsic ability of HDL particles to promote either cellular free cholesterol efflux from macrophages or to deliver CE to hepatic cells.

23 citations


Proceedings ArticleDOI
01 Dec 2012
TL;DR: In this article, a dual-gated transistor with a graphene channel irradiated with He ion beams can have a transport gap of up to 380 meV and an on-off ratio up to 103 at 200 K.
Abstract: We found that a transistor with a graphene channel irradiated with He ion beams can have a transport gap of up to 380 meV. We made novel dual-gated transistors using such a channel and obtained an on-off ratio up to 103 at 200 K. This novel device has a channel region between dual gates, and the polarity of the transistor (n- or p-type) can be electrostatically reversed by simply flipping the bias polarity of one of the dual gates.

23 citations


Journal ArticleDOI
TL;DR: The results obtained in the present study suggest that serum RBP4 concentrations in patients with type 2 diabetes may be associated with diabetes-related renal dysfunction and imbalances in lipid metabolism.
Abstract: How to cite this article Park H, Green MH & Shaffer ML (2012) Association between serum retinol-binding protein 4 concentrations and clinical indices in subjects with type 2 diabetes: a meta-analysis J Hum Nutr Diet 25, 300–310 Abstract Background: Retinol-binding protein (RBP4) is an adipokine that may be important in type 2 diabetes Previous studies have examined the association between serum RBP4 concentrations and clinical indices in patients with type 2 diabetes, although the results obtained have been inconsistent We conducted a meta-analysis aiming to investigate the association between serum RBP4 concentrations and clinical indicators of diabetes, renal function, metabolic syndrome and obesity in subjects with type 2 diabetes Methods: MEDLINE, EMBASE and CINAHL databases were searched from 2005 through November 2011, and the search identified 21 clinical variables from seven studies (total n = 1406) For each variable, summary correlation coefficients (rs) were estimated using a random-effects meta-analysis Results: None of the diabetes markers were correlated with serum RBP4 concentrations in subjects with type 2 diabetes, whereas all of the renal function markers and many metabolic syndrome markers were significantly correlated Summary correlation coefficients and 95% confidence intervals (CIs) were −036 (95% CI = −051 to −018) for creatinine clearance, −039 (95% CI = −044 to −033) for estimated glomerular filtration rate and 053 (95% CI = 030–071) for creatinine concentration In addition, plasma triglyceride concentrations (rs = 022; 95% CI = 011–032), plasma total cholesterol concentrations [rs = 014 (95% CI = 005–023)] and low-density lipoprotein cholesterol level (rs = 014; 95% CI = 002–025) were positively correlated with serum RBP4 concentrations Conclusions: The results obtained in the present study suggest that serum RBP4 concentrations in patients with type 2 diabetes may be associated with diabetes-related renal dysfunction and imbalances in lipid metabolism

11 citations



Journal ArticleDOI
TL;DR: In this paper, a revue fait le point sur les connaissances concernant the fibrose du tissu adipeux lors du developpement de lobesite.