Showing papers by "Instituto Politécnico Nacional published in 1999"

Presynaptic inhibition in the vertebrate spinal cord revisited.

Abstract: The present review examines the experimental evidence supporting the existence of central mechanisms able to modulate the synaptic effectiveness of sensory fibers ending in the spinal cord of vertebrates. The first section covers work on the mode of operation and the synaptic mechanisms of presynaptic inhibition, in particular of the presynaptic control involving axo-axonic synapses made by GABAergic interneurons with the terminal arborizations of the afferent fibers. This includes reviewing of the ionic mechanisms involved in the generation of primary afferent depolarization (PAD) by GABAergic synapses, the ultrastructural basis underlying the generation of PAD, the relationship between PAD and presynaptic inhibition, the conduction of action potentials in the terminal arborizations of the afferent fibers, and the modeling of the presynaptic inhibitory synapse. The second section of the review deals with the functional organization of presynaptic inhibition. This includes the segmental and descending presynaptic control of the synaptic effectiveness of group-I and group-II muscle afferents, the evidence dealing with the local character of PAD as well as the differential inhibition of PAD in selected collaterals of individual muscle-spindle afferents by cutaneous and descending inputs. This section also examines observations on the presynaptic modulation of large cutaneous afferents, including the modulation of the synaptic effectiveness of thin myelinated and unmyelinated cutaneous fibers and of visceral afferents, as well as the presynaptic control of the synaptic actions of interneurons and descending tract neurons. The third section deals with the changes in PAD occurring during sleep and fictive locomotion in higher vertebrates and with the changes of presynaptic inhibition in humans during the execution of a variety of voluntary movements. In the final section, we examine the non-synaptic presynaptic modulation of transmitter release, including the possibility that the intraspinal endings of primary afferents also release colocalized peptides in a similar way as in the periphery. The outcome of the studies presently reviewed is that intraspinal terminals of sensory fibers are not hard-wired conductors of the information generated in their peripheral sensory receptors, but dynamic systems that convey information that can be selectively addressed by central mechanisms to specific neuronal targets. This central control of information flow in peripheral afferents appears to play an important role in the generation of integrated movements and processing of sensory information, including nociceptive information.

Worldwide large-scale fluctuations of sardine and anchovy populations

Abstract: Decade-scale regimes of sardine Sardinops sagax and anchovy Engraulis spp. have been observed in the productive coastal waters of the North-Western, North-Eastern and South-Eastern Pacific and the South-Eastern Atlantic. In each of these systems, the two genera fluctuate out of phase with each other. The subdominant genus may initiate a recovery while the other species is still abundant, so population growth is not necessarily a response to a vacant niche. Rather, it appears to be triggered by formation of one or a few powerful year-classes. At high population levels, quality of sardine and their eggs decreased in Japan, leading to decreased production and survival of eggs, poor year-classes and stock collapse. Excessive fishing of strong year-classes early in the recovery stage may prevent a species from assuming dominance, so influencing the natural succession of species. This may greatly alter the structure and functioning of an ecosystem. For example, a mesopelagic forage fish may replace an epipelagi...

Limiting performance of block-fading channels with multiple antennas

Abstract: We derive the performance limits of a radio system consisting of a transmitter with t antennas and a receiver with r antennas, a block-fading channel with additive white Gaussian noise (AWGN), delay and transmit-power constraints, and perfect channel-state information available at both the transmitter and the receiver. Because of a delay constraint, the transmission of a codeword is assumed to span a finite (and typically small) number M of independent channel realizations; therefore, the relevant performance limits are the information outage probability and the "delay-limited" (or "nonergodic") capacity. We derive the coding scheme that minimizes the information outage probability. This scheme can be interpreted as the concatenation of an optimal code for the AWGN channel without fading to an optimal beamformer. For this optimal scheme, we evaluate minimum-outage probability and delay-limited capacity. Among other results, we prove that, for the fairly general class of regular fading channels, the asymptotic delay-limited capacity slope, expressed in bits per second per hertz (b/s/Hz) per decibel of transmit signal-to-noise ratio (SNR), is proportional to min (t,r) and independent of the number of fading blocks M. Since M is a measure of the time diversity (induced by interleaving) or of the frequency diversity of the system, this result shows that, if channel-state information is available also to the transmitter, very high rates with asymptotically small error probabilities are achievable without the need of deep interleaving or high-frequency diversity. Moreover, for a large number of antennas, delay-limited capacity approaches ergodic capacity.

Specification and implementation of exceptions in workflow management systems

Abstract: Although workflow management systems are most applicable when an organization follows standard business processes and routines, any of these processes faces the need for handling exceptions, i.e., asynchronous and anomalous situations that fall outside the normal control flow.In this paper we concentrate upon anomalous situtations that, although unusual, are part of the semantics of workflow applications, and should be specified and monitored coherently; in most real-life applications, such exceptions affect a significant fraction of workflow cases. However, very few workflow management systems are integrated with a highly expressive language for specifying this kind of exception and with a system component capable of handling it. We present Chimera-Exc, a language for the specification of exceptions for workflows based on detached active rules, and then describe the architecture of a system, called FAR, that implements Chimera-Exc and integrates it with a commercial workflow management system and database server. We discuss the main issues that were solved by our implementation, and report on the performance of FAR. We also discuss design criteria for exceptions in light of the formal properties of their execution. Finally, we focus on the portability of FAR on its unbundling to a generic architecture with detached active rules.

Vibrios associated with Litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts.

Abstract: Several bacteriological surveys were performed from 1994 to 1996 at different Litopenaeus vannamei hatcheries (in Ecuador) and shrimp farms (in Mexico). Samples were taken from routine productions of healthy and diseased L. vannamei larvae, postlarvae, and their culture environment and from healthy and diseased juveniles and broodstock. In Ecuador, the dominant bacterial flora associated with shrimp larvae showing symptoms of zoea 2 syndrome, mysis mold syndrome, and bolitas syndrome has been determined. Strains were characterized by Biolog metabolic fingerprinting and identified by comparison to a database of 850 Vibrio type and reference strains. A selection of strains was further genotypically fine typed by AFLP. Vibrio alginolyticus is predominantly present in all larval stages and is associated with healthy nauplius and zoea stages. AFLP genetic fingerprinting shows high genetic heterogeneity among V. alginolyticus strains, and the results suggest that putative probiotic and pathogenic strains each have specific genotypes. V. alginolyticus was found to be associated with larvae with the zoea 2 syndrome and the mysis mold syndrome, while different Vibrio species (V. alginolyticus and V. harveyi) are associated with the bolitas syndrome. V. harveyi is associated with diseased postlarvae, juveniles, and broodstock. The identities of the strains identified as V. harveyi by the Biolog system could not be unambiguously confirmed by AFLP genomic fingerprinting. Vibrio strain STD3-988 and one unidentified strain (STD3-959) are suspected pathogens of only juvenile and adult stages. V. parahaemolyticus, Photobacterium damselae, and V. mimicus are associated with juvenile and adult stages.

Relationship between Na(+),K(+)-ATPase and cell attachment

Abstract: A prolonged ouabain blockade of the Na(+),K(+)-ATPase detaches cells from each other and from the substrate. This suggests the existence of a link between pump (P) and attachment (A). In the present work, we report that MDCK-W cells treated with ouabain increase tyrosine phosphorylation and content of active MAP kinase, redistribute molecules involved in cell attachment (occludin, ZO-1, desmoplakin, cytokeratin, alpha-actinin, vinculin and actin), and detach. Genistein and UO126, inhibitors of protein tyrosine kinase and of MAP kinase kinase, respectively, block this detachment. The content of P190(Rho-GAP), a GTPase activating protein of the Rho small G-protein subfamily, is increased by ouabain, suggesting that both the Rho/Rac and MAPK pathways are involved. Another clone of MDCK cells whose Na(+),K(+)-ATPase has a negligible affinity for the drug, show none of the effects described for MDCK-W and remain attached. Ma104 cells, a line that has a high affinity for ouabain and stops pumping, fail to modify phosphorylation, as well as the pattern of distribution of attaching molecules, and remain in the monolayer. Taken together, these results suggest that there is a mechanism (P-->A) that transduces a blockade of the pump in a detachment of the cell from neighbors and substrate, in which Ma104 cells are faulty.

Recent advances in the enantioselective synthesis of beta-amino acids.

Abstract: The introductory section of this review presents some of the currently most compelling beta-amino acid targets, according to their structural types: alpha- and beta-aryl substituted, olefinic and alkynyl, alpha, alpha- and alpha,beta-disubstituted, cyclic and conformationally restricted, fluorine-containing, and phosphonic analogous beta-amino acids. The main section highlights some of the very new (1996-1998), promising methodology for the enantioselective synthesis of beta-amino acids, with especial emphasis on catalytic and enzymatic processes, as well as methods based on "chiral pool", self-regeneration of stereogenic centers , diastereoselective nucleophilic additions to prochiral double bonds, and enantioselective reactions in the presence of chiral additives.

A system theory approach for designing cryptosystems based on hyperchaos

Abstract: In this paper a general methodology for designing chaotic and hyperchaotic cryptosystems is developed. The basic idea is to make the decrypter a nonlinear observer for the state of the encrypter. Referring to this concept, some propositions are given which enable the plaintext to be retrieved if proper structural properties of the chaotic system hold. The proposed tool proves to be powerful and flexible, since a wide class of cryptosystems can be designed by exploiting different chaotic and hyperchaotic circuits. The advantages of the suggested approach are illustrated in detail. In particular, the utilization of hyperchaos-based cryptosystems, as well as the increased complexity of the transmitted signal, make a contribution to the development of communication systems with higher security.

Optical Pattern Formation

Abstract: Publisher Summary This chapter focuses on the concept of optical pattern formation. The field of optical pattern formation (OPF) studies the spatial and spatiotemporal phenomena that arise in the structure of electromagnetic field in the planes orthogonal with respect to the direction of propagation. Most theoretical treatments of the interaction between matter and radiation introduce the plane wave approximation—that is, they assume that the electric field is uniform in each transverse plane. However, the field of OPF studies mainly the interaction with nonlinear media, where the phenomena emerge spontaneously as a consequence of an instability; another name that is commonly used to designate OPF is “transverse nonlinear optics.” Historically, the broad interest in OPF emerged as a natural evolution of the previous development of the field of optical instabilities and chaos, when the main attention shifted gradually from purely temporal effects to spatio-temporal phenomena. For both the fields of optical instabilities and OPF, continuous inspiration arose from the formulation of general disciplines as Haken's synergetics or Prigogine's theory of dissipative structures

Entamoeba histolytica : a novel cysteine protease and an adhesin form the 112 kDa surface protein.

Abstract: Summary Here, we present evidence that a cysteine protease (EhCP112) and a protein with an adherence domain (EhADH112) form the Entamoeba histolytica 112 kDa adhesin. Immunoelectron microscopy and immunofluorescence assays using monoclonal antibodies (mAbAdh) revealed that, during phagocytosis, the adhesin is translocated from the plasma membrane to phagocytic vacuoles. mAbAdh inhibited 54% adherence, 41% phagocytosis, and 35% and 62% destruction of MDCK cell monolayers by live trophozoites and their extracts respectively. We cloned a 3587 bp DNA fragment (Eh112 ) with two open reading frames (ORFs) separated by a 188 bp non-coding region. The ORF at the 58 end (Ehcp112 ) encodes a protein with a cysteine protease active site, a transmembranal segment and an RGD motif. The second ORF (Ehadh112 ) encodes a protein recognized by mAbAdh with three putative transmembranal segments and four glycosylation sites. Northern blot, primer extension and Southern blot experiments revealed that Ehcp112 and Ehadh112 are two adjacent genes in DNA. Ehcp112 and Ehadh112 genes were expressed in bacteria. The recombinant peptides presented protease activity and inhibited adherence and phagocytosis, respectively, and both were recognized by mAbAdh. The EhCP112 and EhADH112 peptides could be joined by covalent or strong electrostatic forces, which are not broken during phagocytosis.

5-Lump kinetic model for gas oil catalytic cracking

Abstract: A new 5-lump kinetic model is proposed to describe the gas oil catalytic cracking (FCC) process. The model contains eight kinetic constants, including one for catalyst deactivation, taking into account LPG (combined C 3 –C 4 ), dry gas (C 2 and lighter) and coke yields separately from other lumps (unconverted gas oil and gasoline). Apparent activation energies were determined from experiments obtained in a microactivity reactor (MAT) at temperatures: 480°C, 500°C and 520°C; for a catalyst-to-oil ratio of 5 using vacuum gas oil and equilibrium catalyst, both recovered from an industrial FCC unit. Product yields predicted by this model show good agreement with experimental data.

Magnetic hysteresis based on dipolar interactions in granular magnetic systems

Abstract: The magnetic hysteresis of granular magnetic systems is investigated in the high-temperature limit $(T\ensuremath{\gg}$ blocking temperature of magnetic nanoparticles). Measurements of magnetization curves have been performed at room temperature on various samples of granular bimetallic alloys of the family ${\mathrm{Cu}}_{100\ensuremath{-}x}{\mathrm{Co}}_{x} (x=5--20 \mathrm{at}.%)$ obtained in ribbon form by planar flow casting in a controlled atmosphere, and submitted to different thermal treatments. The loop amplitude and shape, which are functions of sample composition and thermal history, are studied taking advantage of a novel method of graphical representation, particularly apt to emphasize the features of thin, elongated loops. The hysteresis is explained in terms of the effect of magnetic interactions of the dipolar type among magnetic-metal particles, acting to hinder the response of the system of moments to isothermal changes of the applied field. Such a property is accounted for in a mean-field scheme, by introducing a memory term in the argument of the Langevin function which describes the anhysteretic behavior of an assembly of noninteracting superparamagnetic particles. The rms field arising from the cumulative effect of dipolar interactions is linked by the theory to a measurable quantity, the reduced remanence of a major symmetric hysteresis loop. The theory's self-consistence and adequacy have been properly tested at room temperature on all examined systems. The agreement with experimental results is always striking, indicating that at high temperatures the magnetic hysteresis of granular systems is dominated by interparticle, rather than single-particle, effects. Dipolar interactions seem to fully determine the magnetic hysteresis in the high-temperature limit for low Co content $(xl~10).$ For higher concentrations of magnetic metal, the experimental results indicate that additional hysteretic mechanisms have to be introduced.

Study of hydraulic transients in hydropower plants through simulation of nonlinear model of penstock and hydraulic turbine model

Abstract: Study of hydraulic transients in hydropower plants specifically in hydraulic turbine units, with penstock, spiral case and draft tube-is necessary due to power and frequency oscillations which normally occur in generator units, mainly during load rejections. In turn, this causes large pressure and subpressure oscillations in turbine hydraulic systems and must be evaluated to avoid mechanical failures. In this context, this work analyses discrete hydraulic systems with emphasis on hydraulic parameter analysis. The simulation used was developed based on analog mathematical models of transient phenomenon equations and on a hydro turbine model. The results were obtained by using a nonlinear analog-digital simulation method. Comparisons between results of a theoretical simulation of a literature example using the characteristics method and the results obtained with the present method are presented. Theoretical simulation results obtained from a 360 MW turbine and experimental results are also confronted.

Non parabolic band transport in semiconductors: closure of the moment equations

Abstract: The problem of the closure of the moment equations of the semiconductor Boltzmann equation is studied in the framework of the Kane dispersion relation (therefore avoiding the limitations of the parabolic band approximation). By using the maximum entropy ansatz for the closure one obtains, in the limit of small anisotropy, explicit constitutive relations for the stress tensor and the flux of energy flux tensor. The results obtained are in remarkable agreement with those arising from Monte Carlo simulations.

Selection and characterization of a proteo-chitinolytic strain of Bacillus thuringiensis, able to grow in shrimp waste media

Abstract: This paper reports the selection and characterization of Bacillus thuringiensis strains, with ability to grow in a proteo-chitinaceous substrate (milled shrimp waste) as the sole ingredient. Selected strains were able to produce crystal proteins, as well as proteases and chitinases as fermentation by-products. By a preliminary, qualitative screening of 152 B. thuringiensis strains, grown on media rich in protein and chitin, eight strains were selected. These strains were cultured in a liquid medium containing milled shrimp waste and their kinetics of protease production were followed. The two most active proteolytic strains (Bt-103 and Bt-112) were characterized by their crystal protein content, plasmid profiles, crystal ultrastructure, and toxicity towards Manduca sexta, Aedes aegypti and Leptinotarsa texana. The only activity recorded in these species was moderate toxicity of strain Bt-112 against Manduca sexta first instar larvae, as well as the highest proteolytic and chitinolytic activities. Its bipyramidal crystals were associated with semi-cuboidal inclusions and although its crystal proteins were similar to those of B. thuringiensis kurstaki (HD-1), its plasmid content was quite different. Serotyping of Bt-112 indicated that it belongs to serovar. tolworthi. Further studies with a similar strategy might render more strains with ability to grow in a rich waste by-product like the shrimp waste, which may show not only higher insecticidal activity, but also with the ability to produce extracellular enzymes with biotechnological applications.

Delaying physical register allocation through virtual-physical registers

Abstract: Register file access time represents one of the critical delays of current microprocessors, and it is expected to become more critical as future processors increase the instruction window size and the issue width. This paper presents a novel physical register management scheme that allows for a late allocation (at the end of execution) of registers. We show that it can provide significant savings in number of registers and thus, it can significantly shorten the register file access time. The approach is based on virtual-physical registers, which we presented in a previous work, extended with a new register allocation policy. This policy consists of an on-demand allocation in order to maximize the register usage, combined with a stealing mechanism that prevents older instruction from being delayed by younger ones. This shortens the average number of cycles that each physical register is allocated, and allows for an early execution of instructions since they can obtain a physical register for its destination earlier than with the conventional scheme. Early execution is especially beneficial for branches and memory operations, since the former can be resolved earlier and the latter can prefetch their data in advance.

Myoelectric activation pattern during gait in total knee replacement: relationship with kinematics, kinetics, and clinical outcome

Abstract: Gait usually presents an excellent improvement after total knee replacement. Nevertheless, some abnormalities persist even after a long period of time. The abnormal knee patterns have been attributed to several possible causes, such as implant geometry and surgical technique, posterior cruciate ligament sparing/sacrificing, preoperative "stiff-knee" pattern due to pain and altered biomechanics, weakness of the extensor muscles, preoperative arthritic pattern, proprioceptive deficiency, and multijoint degenerative involvement. Cocontraction of the knee flexors and extensors is a common strategy adopted to reduce strain and shear forces at the joint, but it increases compressive forces and joint loading. Even in patients with an excellent functional score, the duration of the implant may be compromised by an altered neuromuscular control of the knee. Here, the authors report a single case study carried out over two years on a patient that underwent total knee replacement. The aim of this work is to show that quantitative gait analysis is essential to augment the understanding of the mechanisms underlying gait, thus enabling clinicians to adapt the rehabilitation program to the specific patient. Although the limits of single case reports are obvious, the authors believe that this evaluation methodology could be beneficial for assessing the effectiveness of rehabilitation programs aimed at achieving an active control of the knee during gait through a correct muscular activation pattern.

Size-dependent dynamics of coherent acoustic phonons in nanocrystal quantum dots

Abstract: Confined acoustic phonons in InAs nanocrystals are observed in the time domain by femtosecond pump-probe spectroscopy. The size dependence of both frequency and damping time is investigated for InAs. The frequency of the discrete modes varies between 18 and 30 ${\mathrm{cm}}^{\mathrm{\ensuremath{-}}1}$ for a nanocrystal radius ranging from 28 to 12 \AA{}, and the dependence deviates from the expected $1/R$ behavior at small sizes. The damping rate is found to vary linearly with $1/R,$ suggesting coupling to the matrix through the particle surface as a main damping route. This is corroborated by observing faster damping for the acoustic mode in solution compared to a polymer environment. The coupling strength is found to depend on material parameters: in CdSe nanocrystals, weaker coupling is observed. The role of acoustic phonon coupling in the dephasing dynamics of semiconductor nanocrystals is discussed.