Instituto Politécnico Nacional

About: Instituto Politécnico Nacional is a education organization based out in Mexico City, Mexico. It is known for research contribution in the topics: Population & Context (language use). The organization has 43351 authors who have published 63315 publications receiving 938532 citations. The organization is also known as: Instituto Politécnico Nacional & Instituto Politecnico Nacional.

Entamoeba histolytica : a novel cysteine protease and an adhesin form the 112 kDa surface protein.

Abstract: Summary Here, we present evidence that a cysteine protease (EhCP112) and a protein with an adherence domain (EhADH112) form the Entamoeba histolytica 112 kDa adhesin. Immunoelectron microscopy and immunofluorescence assays using monoclonal antibodies (mAbAdh) revealed that, during phagocytosis, the adhesin is translocated from the plasma membrane to phagocytic vacuoles. mAbAdh inhibited 54% adherence, 41% phagocytosis, and 35% and 62% destruction of MDCK cell monolayers by live trophozoites and their extracts respectively. We cloned a 3587 bp DNA fragment (Eh112 ) with two open reading frames (ORFs) separated by a 188 bp non-coding region. The ORF at the 58 end (Ehcp112 ) encodes a protein with a cysteine protease active site, a transmembranal segment and an RGD motif. The second ORF (Ehadh112 ) encodes a protein recognized by mAbAdh with three putative transmembranal segments and four glycosylation sites. Northern blot, primer extension and Southern blot experiments revealed that Ehcp112 and Ehadh112 are two adjacent genes in DNA. Ehcp112 and Ehadh112 genes were expressed in bacteria. The recombinant peptides presented protease activity and inhibited adherence and phagocytosis, respectively, and both were recognized by mAbAdh. The EhCP112 and EhADH112 peptides could be joined by covalent or strong electrostatic forces, which are not broken during phagocytosis.

Cooperative frequency locking and stationary spatial structures in lasers

Abstract: We investigate the spontaneous emergence of transverse patterns in lasers by using both the standard two-level model and the so-called cubic approximation, which is generally valid in the threshold regions. The stationary intensity configurations fall into two distinct classes. The first includes solutions of the single-mode type with the frequency and spatial structure of one of the transverse resonances. The solutions of the second group involve the simultaneous oscillation of several cavity modes, operating in such a way as to produce a stationary intensity profile. The stationary character of these multimode configurations emerges from the fact that the transverse modes of the resonator lock onto a common frequency during the nonlinear transient. We call this phenomenon cooperative frequency locking.

A “re-vitalized” Greenwood and Williamson model of elastic contact between fractal surfaces

Abstract: Greenwood and Williamson in 1966 (GW) proposed a theory of elastic contact mechanics of rough surfaces which is today the foundation of many theories in tribology (friction, adhesion, thermal and electrical conductance, wear, etc.). However, the theory has periodically received criticisms for the “resolution-dependence” of the asperity features. Greenwood himself has recently concluded that: “The introduction by Greenwood and Williamson in 1966 of the definition of a ‘peak’ as a point higher than its neighbours on a profile sampled at a finite sampling interval was, in retrospect, a mistake, although it is possible that it was a necessary mistake” [Greenwood and Wu, 2001. Surface roughness and contact: an apology. Meccanica 36 (6), 617–630]. We propose a “discrete” version of the GW model, keeping the approximation of a surface by quadratic functions near summits, where the summit arrangement is found from numerical realizations or real surfaces scans. The contact is then solved either summing the Hertzian relationships, or considering interaction effects to the first-order in a very efficient algorithm. We conduct experiments on Weierstrass–Mandelbrot fractal surfaces, concluding that: (1) the real contact area–load relationship is well captured by the original GW theoretical model, once the correct mean radius is used. The relationship is robust and shows relatively little scatter; (2) the conductance–load relationship is vice versa only approximately given by the original GW theoretical model. Significant deviations from linearity and significant scatter seem to be found, particularly at low fractal dimensions; (3) the load, area and conductance dependences with separation show significant dependence on the actual phase arrangements, and hence significant scatter at large separations. Effect of interaction is seen strongly at low separations, where scatter is minimal. The discrete GW model permits to include these effects, except when the asperity description breaks down. Refinements of the original GW theory using the full random process theory (such as that by Bush Gibson and Thomas, BGT) result only in small improvements with a significant additional complication. However, the BGT relationship between contact area and load at low loads is more accurate than the more recent theory by Persson. The distribution derived from the original GW theory has been obtained, and shown to be closer to the numerical results than that predicted by the Persson model, even if the area error is removed. It is concluded that the original GW theory deserves the general success received so far, since the resolution-dependence of geometrical features is an intrinsic feature of “fractals” but not a problem for the GW theory, when interaction effects are included.