Jagiellonian University

About: Jagiellonian University is a education organization based out in Krakow, Poland. It is known for research contribution in the topics: Population & Catalysis. The organization has 17438 authors who have published 44092 publications receiving 862633 citations. The organization is also known as: Academia Cracoviensis & Akademia Krakowska.

Enhanced expression of the leukotriene C(4) synthase due to overactive transcription of an allelic variant associated with aspirin-intolerant asthma.

Abstract: Aspirin-intolerant asthma (AIA), a distinct clinical syndrome affecting about 10% of adult asthmatics, appears to be unusually dependent on cysteine leukotriene (cys-LT) overproduction by pulmonary eosinophils. The gene coding for leukotriene (LT) C4 synthase (LTC4S), the enzyme controlling cys-LT biosynthesis, exists as two common alleles distinguished by an A to C transversion at a site 444 nucleotides upstream of the translation start. We tested the hypothesis that this single nucleotide polymorphism (SNP) affects binding of transcription factors and influences the transcription rate, predisposing to AIA. Gel shift assay studies revealed that the − 444C allele, conferring an activator protein-2 binding sequence, is an additional target for a transcription factor of histone H4 consensus. Introduction of the H4TF-2 decoy oligonucleotide into LTC4S-positive, differentiated HL-60 cells decreased accumulation of LTC4 to 68%. Transfection of COS-7 with promoter construct increased expression of β -galactosid...

The Balbiani body and germ cell determinants: 150 years later.

Abstract: Publisher Summary This chapter describes the Balbiani body (Bb) in various animal species. The most comprehensive ultrastructural and molecular studies on the origin, composition, and function of Bb and its relationship to the germplasm have been done for the oocytes and embryos of Xenopus. Various RNAs and proteins have been discovered localized in the mitochondrial cloud (MC) and in the germplasm in Xenopus oocytes and embryos. The chapter discusses two major pathways of RNA localization in Xenopus: (1) Message Transport Organizer (METRO) or early pathway-localizing RNAs, (2) Late or Vg1 pathway-localizing RNAs. Emphasis is given on the localization of METRO pathway RNAs within the MC and the germplasm islands in the embryo. The chapter discusses the ultrastructural, molecular, and functional studies that have been carried out on polar granules and germ cells of Drosophila melanogaster . It reveals that the molecular composition of pole plasm, polar granules, nuage, and sponge bodies in Drosophila has been deduced from mutational and functional analyses and indirect genetic approaches.

Performance of missing transverse momentum reconstruction with the ATLAS detector using proton-proton collisions at s√ = 13 TeV

Abstract: The performance of the missing transverse momentum ( ETmiss ) reconstruction with the ATLAS detector is evaluated using data collected in proton-proton collisions at the LHC at a centre-of-mass energy of 13 TeV in 2015. To reconstruct ETmiss , fully calibrated electrons, muons, photons, hadronically decaying τ-leptons , and jets reconstructed from calorimeter energy deposits and charged-particle tracks are used. These are combined with the soft hadronic activity measured by reconstructed charged-particle tracks not associated with the hard objects. Possible double counting of contributions from reconstructed charged-particle tracks from the inner detector, energy deposits in the calorimeter, and reconstructed muons from the muon spectrometer is avoided by applying a signal ambiguity resolution procedure which rejects already used signals when combining the various ETmiss contributions. The individual terms as well as the overall reconstructed ETmiss are evaluated with various performance metrics for scale (linearity), resolution, and sensitivity to the data-taking conditions. The method developed to determine the systematic uncertainties of the ETmiss scale and resolution is discussed. Results are shown based on the full 2015 data sample corresponding to an integrated luminosity of 3.2fb-1 .