Jagiellonian University

About: Jagiellonian University is a education organization based out in Krakow, Poland. It is known for research contribution in the topics: Population & Catalysis. The organization has 17438 authors who have published 44092 publications receiving 862633 citations. The organization is also known as: Academia Cracoviensis & Akademia Krakowska.

The VIMOS Public Extragalactic Redshift Survey (VIPERS). Full spectroscopic data and auxiliary information release (PDR-2)

Abstract: We present the full public data release (PDR-2) of the VIMOS Public Extragalactic Redshift Survey (VIPERS), performed at the ESO VLT. We release redshifts, spectra, CFHTLS magnitudes and ancillary information (as masks and weights) for a complete sample of 86 775 galaxies (plus 4732 other objects, including stars and serendipitous galaxies); we also include their full photometrically-selected parent catalogue. The sample is magnitude limited to i AB ≤ 22.5, with an additional colour-colour pre-selection devised as to exclude galaxies at z h 3 Mpc-3 . The total area spanned by the final data set is ≃ 23.5 deg2 , corresponding to 288 VIMOS fields with marginal overlaps, split over two regions within the CFHTLS-Wide W1 and W4 equatorial fields (at RA ≃ 2 and ≃ 22 h, respectively). Spectra were observed at a resolution R = 220, covering a wavelength range 5500−9500 A. Data reduction and redshift measurements were performed through a fully automated pipeline; all redshift determinations were then visually validated and assigned a quality flag. Measurements with a quality flag ≥ 2 are shown to have a confidence level of 96% or larger and make up 88% of all measured galaxy redshifts (76 552 out of 86 775), constituting the VIPERS prime catalogue for statistical investigations. For this sample the rms redshift error, estimated using repeated measurements of about 3000 galaxies, is found to be σ z = 0.00054(1 + z ). All data are available at http://vipers.inaf.it and on the ESO Archive.

Entry of Human Coronavirus NL63 into the Cell

Abstract: The first steps of human coronavirus NL63 (HCoV-NL63) infection were previously described. The virus binds to target cells by use of heparan sulfate proteoglycans and interacts with the ACE2 protein. Subsequent events, including virus internalization and trafficking, remain to be elucidated. In this study, we mapped the process of HCoV-NL63 entry into the LLC-Mk2 cell line and ex vivo three-dimensional (3D) tracheobronchial tissue. Using a variety of techniques, we have shown that HCoV-NL63 virions require endocytosis for successful entry into the LLC-MK2 cells, and interaction between the virus and the ACE2 molecule triggers recruitment of clathrin. Subsequent vesicle scission by dynamin results in virus internalization, and the newly formed vesicle passes the actin cortex, which requires active cytoskeleton rearrangement. Finally, acidification of the endosomal microenvironment is required for successful fusion and release of the viral genome into the cytoplasm. For 3D tracheobronchial tissue cultures, we also observed that the virus enters the cell by clathrin-mediated endocytosis, but we obtained results suggesting that this pathway may be bypassed.IMPORTANCE Available data on coronavirus entry frequently originate from studies employing immortalized cell lines or undifferentiated cells. Here, using the most advanced 3D tissue culture system mimicking the epithelium of conductive airways, we systematically mapped HCoV-NL63 entry into susceptible cells. The data obtained allow for a better understanding of the infection process and may support development of novel treatment strategies.