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Institution

Jagiellonian University

EducationKrakow, Poland
About: Jagiellonian University is a education organization based out in Krakow, Poland. It is known for research contribution in the topics: Population & Catalysis. The organization has 17438 authors who have published 44092 publications receiving 862633 citations. The organization is also known as: Academia Cracoviensis & Akademia Krakowska.


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Journal ArticleDOI
TL;DR: It was shown that, in the oral epithelial cell line, PAR activation by the bacterial protease-stimulated secretion of interleukin-6 suggests a mechanism whereby cysteine proteases from P. gingivalis might mediate inflammatory events associated with periodontal disease on first contact with a primary barrier of cells.
Abstract: Periodontitis is a chronic inflammatory disease affecting oral tissues. Oral epithelial cells represent the primary barrier against bacteria causing the disease. We examined the responses of such cells to an arginine-specific cysteine proteinase (RgpB) produced by a causative agent of periodontal disease, Porphyromonas gingivalis. This protease caused an intracellular calcium transient in an oral epithelial cell line (KB), which was dependent on its enzymatic activity. Since protease-activated receptors (PARs) might mediate such signaling, reverse transcription-PCR was used to characterize the range of these receptors expressed in the KB cells. The cells were found to express PAR-1, PAR-2, and PAR-3, but not PAR-4. In immunohistochemical studies, human gingival epithelial cells were found to express PAR-1, PAR-2, and PAR-3 on their surface, but not PAR-4, indicating that the cell line was an effective model for the in vivo situation. PAR-1 and PAR-2 expression was confirmed in intracellular calcium mobilization assays by treatment of the cells with the relevant receptor agonist peptides. Desensitization experiments strongly indicated that signaling of the effects of RgpB was occurring through PAR-1 and PAR-2. Studies with cells individually transfected with each of these two receptors confirmed that they were both activated by RgpB. Finally, it was shown that, in the oral epithelial cell line, PAR activation by the bacterial protease-stimulated secretion of interleukin-6. This induction of a powerful proinflammatory cytokine suggests a mechanism whereby cysteine proteases from P. gingivalis might mediate inflammatory events associated with periodontal disease on first contact with a primary barrier of cells.

245 citations

Journal ArticleDOI
K.-H. Ackermann1, Matteo Agostini2, M. Allardt3, M. Altmann1, E. Andreotti4, A. M. Bakalyarov5, M. Balata, I. R. Barabanov6, M. Barnabe Heider1, M. Barnabe Heider2, N. Barros3, Laura Baudis7, C. Bauer1, N. Becerici-Schmidt1, E. Bellotti8, S. Belogurov6, S. T. Belyaev5, Giovanni Benato7, Alessandro Bettini9, L. B. Bezrukov6, T. Bode2, V.B. Brudanin10, R. Brugnera9, D. Budjáš2, Allen Caldwell1, C. Cattadori8, A. Chernogorov, O. Chkvorets1, F. Cossavella1, A. D Andragora, E. V. Demidova, A. Denisov6, A. di Vacri, A. Domula3, V. G. Egorov10, R. Falkenstein4, A. D. Ferella7, K. Freund4, F. Froborg7, N. Frodyma11, A. M. Gangapshev6, A. M. Gangapshev1, A. Garfagnini9, J. Gasparro, S. Gazzana1, R. Gonzalez de Orduna, P. Grabmayr4, V. I. Gurentsov6, K. N. Gusev10, K. N. Gusev5, K. N. Gusev2, K. K. Guthikonda7, W. Hampel1, A. Hegai4, M. Heisel1, S. Hemmer9, G. Heusser1, Werner Hofmann1, Mikael Hult, L. V. Inzhechik6, L. Ioannucci, J. Janicskó Csáthy2, Josef Jochum4, M. Junker, R. Kankanyan1, S. Kianovsky6, Thomas Kihm1, J. Kiko1, I. V. Kirpichnikov, A. Kirsch1, A. A. Klimenko10, A. A. Klimenko6, A. A. Klimenko1, M. Knapp4, K. T. Knöpfle1, O.I. Kochetov10, V. N. Kornoukhov6, Kevin Kröninger1, V. Kusminov6, M. Laubenstein, A. Lazzaro2, V. I. Lebedev5, B. Lehnert3, D. Lenz1, H. Y. Liao1, Manfred Lindner1, Ivano Lippi, Jing Liu1, X. Liu12, A. Lubashevskiy1, Bayarto Lubsandorzhiev6, A. A. Machado1, Bela Majorovits1, W. Maneschg1, Gerd Marissens, S. Mayer1, G. Meierhofer13, G. Meierhofer4, Igor Nemchenok10, L. Niedermeier4, Stefano Nisi, J. Oehm1, C.M. O'Shaughnessy1, Luciano Pandola, P. Peiffer1, K. Pelczar11, Alberto Pullia14, Stefano Riboldi14, F. Ritter15, F. Ritter4, C. Rossi Alvarez, Cinzia Sada9, M. Salathe1, C. Schmitt4, S. Schönert2, Jochen Schreiner1, J. Schubert1, O. Schulz1, U. Schwan1, B. Schwingenheuer1, H. Seitz1, E. Shevchik10, M. Shirchenko5, M. Shirchenko10, Hardy Simgen1, A.A. Smolnikov1, L. Stanco, F. Stelzer1, H. Strecker1, M. Tarka7, U. Trunk1, C. A. Ur, A. A. Vasenko, S. Vogt1, O. Volynets1, K. von Sturm4, V. Wagner1, M. Walter7, A. Wegmann1, Marcin Wójcik11, E. A. Yanovich6, P. Zavarise, I. Zhitnikov10, S. V. Zhukov5, D. R. Zinatulina10, Kai Zuber3, G. Zuzel11 
TL;DR: The Gerda detector as mentioned in this paper performed a search for neutrinoless double beta decay of 76Ge with the eponymous detector at the Laboratori Nazionali del Gran Sasso and started operation in November 2011.
Abstract: The Gerda collaboration is performing a search for neutrinoless double beta decay of 76Ge with the eponymous detector. The experiment has been installed and commissioned at the Laboratori Nazionali del Gran Sasso and has started operation in November 2011. The design, construction and first operational results are described, along with detailed information from the R&D phase.

245 citations

Journal ArticleDOI
TL;DR: Staphylococcus aureus has several extracellular proteases with proposed roles in virulence and it has been shown that insertional inactivation of sspA or sspB results in significant attenuation of virulence, whilst mutations in aur or scpA do not.
Abstract: Staphylococcus aureus has several extracellular proteases with proposed roles in virulence. SspA (serine protease), SspB (cysteine protease) and Aur (metalloprotease) have been characterized previously and SspA and SspB were found to be cotranscribed. The coding region for the cysteine protease ScpA has been identified and characterized. It is in a probable bi-cistronic operon with scpA located immediately upstream of a coding region for a 108 aa protein that is a specific inhibitor of ScpA. Using primer extension analysis promoters have been mapped and it was found that σ A is the only sigma factor involved in the transcription of scpA, sspABC and aur. The transcription of all the genes occurs maximally at post-exponential phase, being positively regulated by agr (accessory gene regulator) and negatively regulated by sarA (staphylococcal accessory regulator). Furthermore σ B represses transcription from the aur and scp operons similarly to the previously shown effect on ssp [ Horsburgh, M., Aish, J., White, I., Shaw, L., Lithgow, J. & Foster, S. (2002). J Bacteriol 184, 5457–5467 ]. Using mutations in each protease gene the proteolytic cascade of activation has been analysed. Aur, SspA, SspB and ScpA are all produced as zymogens, activated by proteolytic cleavage. Although the metalloprotease, Aur, does catalyse activation of the SspA zymogen, it is not the sole agent capable of conducting this process. Site-directed mutagenesis revealed that Aur is not capable of undergoing auto-proteolysis to achieve activation. The cysteine protease, ScpA, appears to reside outside this cascade of activation, as mature ScpA was observed in the aur, sspA and sspB mutant strains. Using a mouse abscess model, it has been shown that insertional inactivation of sspA or sspB results in significant attenuation of virulence, whilst mutations in aur or scpA do not. It is likely the attenuation observed in the sspA strain is due to polarity on the sspB gene.

244 citations

Journal ArticleDOI
13 Sep 2007-Nature
TL;DR: The detection of a planet orbiting a post-red-giant star demonstrates that planets with orbital distances of less than 2 au can survive the red-Giant expansion of their parent stars.
Abstract: After the initial discoveries fifteen years ago, over 200 extrasolar planets have now been detected. Most of them orbit main-sequence stars similar to our Sun, although a few planets orbiting red giant stars have been recently found. When the hydrogen in their cores runs out, main-sequence stars undergo an expansion into red-giant stars. This expansion can modify the orbits of planets and can easily reach and engulf the inner planets. The same will happen to the planets of our Solar System in about five billion years and the fate of the Earth is matter of debate. Here we report the discovery of a planetary-mass body (Msini = 3.2M_(Jupiter)) orbiting the star V 391 Pegasi at a distance of about 1.7 astronomical units (au), with a period of 3.2 years. This star is on the extreme horizontal branch of the Hertzsprung–Russell diagram, burning helium in its core and pulsating. The maximum radius of the red-giant precursor of V 391 Pegasi may have reached 0.7 au, while the orbital distance of the planet during the stellar main-sequence phase is estimated to be about 1 au. This detection of a planet orbiting a post-red-giant star demonstrates that planets with orbital distances of less than 2 au can survive the red-giant expansion of their parent stars.

244 citations

Journal ArticleDOI
TL;DR: In this paper, the dipole strength distribution above the one-neutron separation energy was measured in the unstable and double-magic Sn isotopes, and the results were deduced from Coulomb dissociation of secondary Sn beams with energies around.
Abstract: The dipole strength distribution above the one-neutron separation energy was measured in the unstable $^{130}\mathrm{Sn}$ and the double-magic $^{132}\mathrm{Sn}$ isotopes. The results were deduced from Coulomb dissociation of secondary Sn beams with energies around $500\text{ }\text{ }\mathrm{MeV}/\mathrm{\text{nucleon}}$, produced by in-flight fission of a primary $^{238}\mathrm{U}$ beam. In addition to the giant dipole resonance, a resonancelike structure (``pygmy resonance'') is observed at a lower excitation energy around 10 MeV exhausting a few percent of the isovector $E1$ energy-weighted sum rule. The results are discussed in the context of a predicted new dipole mode of excess neutrons oscillating out of phase with the core nucleons.

244 citations


Authors

Showing all 17729 results

NameH-indexPapersCitations
Roxana Mehran141137899398
Brad Abbott137156698604
M. Morii1341664102074
M. Franklin134158195304
John Huth131108785341
Wladyslaw Dabrowski12999079728
Rostislav Konoplich12881173790
Michel Vetterli12890176064
Francois Corriveau128102275729
Christoph Falk Anders12673468828
Tomasz Bulik12169886211
Elzbieta Richter-Was11879369127
S. H. Robertson116131158582
S. J. Chen116155962804
David M. Stern10727147461
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
2023162
2022510
20212,769
20202,776
20192,736
20182,735