Janta Vedic College

About: Janta Vedic College is a based out in . It is known for research contribution in the topics: Ionization & Electron ionization. The organization has 46 authors who have published 42 publications receiving 566 citations.

Predicting antimicrobial peptides with improved accuracy by incorporating the compositional, physico-chemical and structural features into Chou's general PseAAC.

Abstract: Antimicrobial peptides (AMPs) are important components of the innate immune system that have been found to be effective against disease causing pathogens. Identification of AMPs through wet-lab experiment is expensive. Therefore, development of efficient computational tool is essential to identify the best candidate AMP prior to the in vitro experimentation. In this study, we made an attempt to develop a support vector machine (SVM) based computational approach for prediction of AMPs with improved accuracy. Initially, compositional, physico-chemical and structural features of the peptides were generated that were subsequently used as input in SVM for prediction of AMPs. The proposed approach achieved higher accuracy than several existing approaches, while compared using benchmark dataset. Based on the proposed approach, an online prediction server iAMPpred has also been developed to help the scientific community in predicting AMPs, which is freely accessible at http://cabgrid.res.in:8080/amppred/. The proposed approach is believed to supplement the tools and techniques that have been developed in the past for prediction of AMPs.

Antinutritional factors and their detoxification in pulses- a review

Abstract: Pulses are relatively a cheaper source of protein than milk, cheese, cashew, almond, meat and fish etc., hence valuable for developing countries. The seeds of pulses are most commonly eaten can be economically stored well for future use. The food values of seeds of pulses is high, have about the some calorific value per unit weight as cereals and are fair sources of some vitamins and minerals. Their protein contents are generally about double that of most cereals. Consumption of pulses is highest in India as compared to other pulses growing countries due to low purchasing power and religious restrictions on non-vegetarian diet. Pulses contain about 18.0 to 32.0% protein and about 1 to 5% fat. Pulses are considerably richer in calcium than most cereals and contain about 100 to 200 mg of calcium per 100 g of grain. They are also considerably rich in iron, thiamine, riboflavin and nicotinic acid as compared to cereals. Young sprouts of pulses like mungbean, mothbean and chickpea are popular foods in some places. Pulses contain several anti-nutritional factors, such as trypsin and chymotrypsin inhibitors, lectins, polyphenols, flatulence factors, lathyrogens, saponins, antihistamines and allergens. The protease inhibitors, lectins and other antinutrients cause toxicity. Heat treatment has been well established to destroy proteinaceous antinutrients, such as protease inhibitors and lectins, but heat treatment destroys some of the amino acids and vitamins as well. For maintaining the nutritional value of food, it is necessary that heating temperature and length of processing do not exceed the optimum temperature required to eliminate the effect of inhibitors. Proteins in pulses are known to interact with lipids, tannins, phytates, flavor compounds and pigments. These interactions occur when pulses are processed and converted into products. It decreases the bioavailability of proteins. Similarly, tannins and phytates interact with minerals and vitamins resulting in a decrease in their bioavailability. Thus, bioavailability of nutrients depends not only on their content in the seed, but also on the interaction of nutrients under various processing conditions. The pulses are subjected to various processing techniques like milling, dehulling, soaking, germination, fermentation and cooking. These processing techniques not only save time, energy and fuel but have several nutritional advantages and produce edible products having a higher nutritional value and lower toxic compound. The degrees of elimination of toxic compound depend on type of pulses and the processing technique.

Study of nano-CdS prepared in methanolic solution and polymer electrolyte matrix

Abstract: This present paper reports the study of cadmium sulphide (CdS) nanoparticles prepared under controlled condition in methanolic solution and also in polyethylene oxide polymer electrolyte matrix. The sulphurations of the cadmium salts were done in situ by a sodium sulphide solution. The concentration of the precursors has been controlled as detailed in the paper. All the preparation and characterization were carried out at room temperature. The nanoparticles were characterized by UV/Vis spectra in the range of 600 to 250 nm. The absence of bulk/micron size particles was noted in either of the system. The absorption edge near 510 nm indicates the presence of bulk CdS, whereas the presence of nanometer-sized particles is also reflected in low wavelength region. The sizes of the particles as well as particle distribution have been estimated using X-ray diffraction and scanning electron microscopy, respectively. A comparison in the spectra has been done for the sample prepared in either of the matrices. The particle size distribution in the polymeric matrix has been found to be more in comparison to that in the methanolic suspension. The polymer is reported to work as a binder, but not as a capping agent.

Enumeration of methanogens with a focus on fluorescence in situ hybridization

Abstract: Methanogens, the members of domain Archaea are potent contributors in global warming. Being confined to the strict anaerobic environment, their direct cultivation as pure culture is quite difficult. Therefore, a range of culture-independent methods have been developed to investigate their numbers, substrate uptake patterns, and identification in complex microbial communities. Unlike other approaches, fluorescence in situ hybridization (FISH) is not only used for faster quantification and accurate identification but also to reveal the physiological properties and spatiotemporal dynamics of methanogens in their natural environment. Aside from the methodological aspects and application of FISH, this review also focuses on culture-dependent and -independent techniques employed in enumerating methanogens along with associated problems. In addition, the combination of FISH with micro-autoradiography that could also be an important tool in investigating the activities of methanogens is also discussed.