Jewish Hospital

About: Jewish Hospital is a(n) healthcare organization based out in Cincinnati, Ohio, United States. It is known for research contribution in the topic(s): Antigen & Population. The organization has 3881 authors who have published 3414 publication(s) receiving 123044 citation(s).

Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine.

Abstract: The highly conserved and ubiquitously expressed 14-3-3 family of proteins bind to a variety of proteins involved in signal transduction and cell cycle regulation. The nature and specificity of 14-3-3 binding is, however, not known. Here we show that 14-3-3 is a specific phosphoserine-binding protein. Using a panel of phosphorylated peptides based on Raf-1, we have defined the 14-3-3 binding motif and show that most of the known 14-3-3 binding proteins contain the motif. Peptides containing the motif could disrupt 14-3-3 complexes and inhibit maturation of Xenopus laevis oocytes. These results suggest that the interactions of 14-3-3 with signaling proteins are critical for the activation of signaling proteins. Our findings also suggest novel roles for serine/threonine phosphorylation in the assembly of protein-protein complexes.