Jewish Hospital

About: Jewish Hospital is a healthcare organization based out in Cincinnati, Ohio, United States. It is known for research contribution in the topics: Antigen & Population. The organization has 3881 authors who have published 3414 publications receiving 123044 citations.

The primary interaction of antibody to components of aspergilli. I. Immunologic and chemical characteristics of a nonprecipitating antigen.

Abstract: The experiments presented in this report describe the preparation of a radiolabeled antigen which can be used in the ammonium sulfate test to measure antibodies against aspergilli. The antigenic components were in the trichloroacetic acid (TCA)-soluble fraction from sonically disrupted aspergilli. The major radiolabeled ligand appeared to be an immunologically nonprecipitating anionic mucopolysaccharide which was insensitive to DNase or RNase, slightly sensitive to pepsin, and very sensitive to periodate oxidation and borohydride reduction. Results of quantitative studies indicate that the radiolabeled antigens had a relatively low affinity for antibody populations produced in hyperimmunized rabbits. Quantitative inhibition experiments as well as immunodiffusion studies demonstrated similarities and differences among species of the genus Aspergillus as well as other fungi. Commercial house dust extracts appear to contain significant amounts of the TCA-soluble aspergilli antigens.

The Composition of Bone. IV. Primary Calcification.

Abstract: Sixteen specimens of normal rat bone were analyzed using the micro-technique described in previous communications.1,2 The age of the rats ranged from 1 day to maturity. A mean value of 1.99 ± 0.05 ...

Rapid postsynthetic destruction of unstable haemoglobin Bushwick.

Abstract: MOST structural variants of human haemoglobin occur in smaller amounts than HbA in the peripheral blood of heterozygous subjects. Abnormal haemoglobins with mutations in the β chain usually comprise 35–45% of the haemoglobin in haemolysates. In contrast, haemoglobins with abnormal α chains generally amount to only 20–25% of the total haemoglobin. This difference in the proportion of α and β chain variants is thought to reflect the fact that in some populations there are two α loci and only one out of four α globin genes is affected in a heterozygote, whereas one out of two β globin alleles is abnormal in a subject with a β varient1,2. Certain unstable haemoglobins with critical alterations in β-chain structure also constitute a much smaller proportion of the total circulating haemoglobin than HbA. The low concentration of these abnormal haemoglobins is the result of accelerated preferential destruction3–7. We report here a new unstable β variant, Hb Bushwick, which was detected in an intact form constituting only 1–2% of the total circulating haemoglobin. Evidence was found for rapid postsynthetic destruction of the abnormal β chain. In spite of this accelerated loss of the product of one of the two β alleles, the affected erythrocytes contained normal amounts of haemoglobin.

Niflumic acid and MSI-2216 reduce TNF-α–induced mucin expression in human airway mucosa

Abstract: Background Human chloride channel, calcium-activated 1 (hCLCA1) has been shown to induce mucin (MUC) gene expression and mucus production in bronchial epithelial cells. Objective To investigate whether blocking hCLCA1 decreases mucus production. Methods Expression of hCLCA1 and mucus was stimulated with TNF-α in human upper airway mucosal explant tissue. MUC5AC mRNA and mucus protein expression was blocked by inhibiting hCLCA1 by using channel blockers (niflumic acid [NFA] and MSI-2216) without and with TNF-α stimulation. Expression of MUC5AC, hCLCA1, and COX-2 mRNA was quantified by using real-time PCR. Mucus protein was assessed by periodic acid Schiff staining. Laser capture microdissection was performed to quantify hCLCA1 and MUC5AC mRNA expression in epithelial cells derived from mucosal explant tissue. Results TNF-α significantly increased MUC5AC and hCLCA1 mRNA as well as mucus and hCLCA1 protein expression in the mucosal explant tissue ( P P Conclusions Unstimulated and TNF-α–induced mucin expression could be decreased by NFA and MSI-2216. Inhibiting hCLCA1 may be a potential new approach to reduce mucus overproduction.