Jewish Hospital

About: Jewish Hospital is a healthcare organization based out in Cincinnati, Ohio, United States. It is known for research contribution in the topics: Antigen & Population. The organization has 3881 authors who have published 3414 publications receiving 123044 citations.

Safety and efficacy of treatment of pediatric cholesteryl ester storage disease with lovastatin.

Abstract: Safety and Efficacy of Treatment of Pediatric Cholesteryl Ester Storage Disease with Lovastatin

Dynamic Sentinel Node Biopsy for Inguinal Lymph Node Staging in Patients with Penile Cancer: A Systematic Review and Cumulative Analysis of the Literature

Abstract: Background Dynamic sentinel node biopsy (DSNB) is used to evaluate the nodal status of patients with clinically node-negative penile carcinoma. Its use is not widespread, and the majority of patients with clinically node-negative disease undergo an elective inguinal lymph node dissection (ILND). However, a prophylactic bilateral ILND is a procedure with approximately 50% of morbidity. The purpose of this review is to evaluate the accuracy of DSNB in penile cancer.

Quantitative analysis of the plain radiographic appearance of aneurysmal bone cysts.

Abstract: RATIONALE AND OBJECTIVES The authors determine the features that distinguish the radiographic appearance of unicameral bone cysts (UBC) from other solitary lesions of bone and to develop an ordered differential of similar-appearing lesions. MATERIALS AND METHODS Seven hundred nine cases of solitary bone lesions, including 40 UBCs, were analyzed according to demographic, anatomic, and plain radiographic features. Vector analysis of both classic and new groups of features was performed to determine the sensitivity and specificity of radiographs for UBC and an ordered differential based on radiographic features. RESULTS The features of the UBCs in this study compared favorably with those in the literature. The male:female ratio was 3.3:1, with an average age of 15 +/- 13 years. Age ranges and lesion diameters were similar for both sexes. Thirty-three of the 40 (83%) UBCs were in long bones, and seven were in the pelvis or calcaneus. All 40 UBCs were geographic, medullary, and lytic. None had an associated soft-tissue mass. Fifty-five percent had pathologic fractures, and 10% had fallen fragment signs. Ninety-eight percent had no cortical break, and 88% had well-defined margins. CONCLUSION Classic descriptions of UBCs can be refined to improve sensitivity without sacrificing specificity. The best vector in this study had a sensitivity of 80% and a specificity of 93% and included these radiographic features: metaphyseal, diaphyseal, or flat bone location, geographic, lytic, medullary-based, no matrix, no satellite lesions, no subarticular extension, no soft-tissue mass, no cortical break, and central location in long bones. An ordered differential diagnosis, starting with the most likely lesion, includes enchondroma, aneurysmal bone cyst, fibrous dysplasia, nonossifying fibroma, Brodie abscess, and chondrosarcoma.

A peripheral mechanism preserves self-tolerance to a secreted protein in transgenic mice.

Abstract: We have examined mechanisms of tolerance to circulating self-proteins in mice that are transgenic for human insulin. Normal, nontransgenic mice develop serum antibody responses when injected with human insulin in CFA; syngeneic transgenic mice do not. B cell responsiveness was assessed by immunizing with human insulin coupled to a T-independent Ag, Brucella abortus. No differences were found in the numbers of insulin-specific splenic plaque-forming cells between transgenic and nontransgenic mice suggesting that insulin-specific B cells are not tolerant in transgenic mice. Similarly, APC from transgenic and nontransgenic mice display no differences in their ability to process and present human insulin to human insulin-specific T cells in vitro. However, marked differences were detected between transgenic and nontransgenic T cells. Lymph node T cells from transgenic mice primed with human insulin provided no detectable helper activity for secondary antibody responses to human insulin whereas, lymph node T cells from nontransgenic mice did. Nevertheless, lymph node T cells from transgenic mice developed significant proliferative responses to human insulin. Lymph node T cells obtained from transgenic and nontransgenic mice were fused to BW5147 and human insulin-specific T cell hybridomas were generated. The fact that human insulin-specific T cell hybridomas were obtained from the transgenic mice suggests that these T cells were not clonally deleted. In addition, APC from transgenic mice did not stimulate human insulin-specific hybridomas from normal mice in the absence of exogenous insulin. We suggest that T cells specific for human insulin are not deleted in the thymus of transgenic mice because APC in the thymus do not bear the requisite levels of endogenous human insulin/Ia complexes. Therefore, we conclude that tolerance in the transgenic mice is preserved by peripheral mechanisms.

Phosphoproteins in Dictyostelium discoideum.

Abstract: The phosphoproteins of Dictyostelium discoideum were compared at different stages of development by polyacrylamide gel electrophoresis. Certain phosphoproteins of vegetative amoebae were conserved while others appeared and disappeared during development. Four major phosphoproteins with apparent subunit molecular weights of 50,000, 47,000, 38,000, and 34,000 disappeared precociously in response to exogenous cAMP. Two membranal phosphoproteins, with apparent subunit molecular weights of 80,000 and 81,000, appeared precociously in response to added cAMP. One of these phosphoproteins, molecular weight of 80,000, has been identified tentatively as the “contact site A” glycoprotein. Another membranal protein, with apparent subunit molecular weight of 42,000, unaffected in its appearance by cAMP, has been identified tentatively as phosphoactin.