Jožef Stefan Institute

About: Jožef Stefan Institute is a facility organization based out in Ljubljana, Slovenia. It is known for research contribution in the topics: Liquid crystal & Dielectric. The organization has 3828 authors who have published 12614 publications receiving 291025 citations.

Oxidative Halogenation with "Green" Oxidants: Oxygen and Hydrogen Peroxide

Abstract: It is difficult to imagine organic chemistry without organo-halogen compounds and the molecular halogens needed for their preparation. The halogens have very different reactivity, with iodine usually requiring some form of activation, while others are reactive and hazardous chemicals. To avoid their use, various modified reagents have been discovered (N-bromo- and N-chlorosuccinimide, Selectfluor..), but halogens are used to prepare these reagents and when they are used the atom economy is poor. A better approach, which is based on biomimetric research on oxidative halogenation in nature, consists of generating the halogenating reagent in situ under acidic conditions from a halide salt. The result of such a reaction has been halogenation with 100 % halogen atom economy. Suitable oxidants for the oxidation of halides are hydrogen peroxide and oxygen.

A survey of processors with explicit multithreading

Abstract: Hardware multithreading is becoming a generally applied technique in the next generation of microprocessors. Several multithreaded processors are announced by industry or already into production in the areas of high-performance microprocessors, media, and network processors.A multithreaded processor is able to pursue two or more threads of control in parallel within the processor pipeline. The contexts of two or more threads of control are often stored in separate on-chip register sets. Unused instruction slots, which arise from latencies during the pipelined execution of single-threaded programs by a contemporary microprocessor, are filled by instructions of other threads within a multithreaded processor. The execution units are multiplexed between the thread contexts that are loaded in the register sets.Underutilization of a superscalar processor due to missing instruction-level parallelism can be overcome by simultaneous multithreading, where a processor can issue multiple instructions from multiple threads each cycle. Simultaneous multithreaded processors combine the multithreading technique with a wide-issue superscalar processor to utilize a larger part of the issue bandwidth by issuing instructions from different threads simultaneously.Explicit multithreaded processors are multithreaded processors that apply processes or operating system threads in their hardware thread slots. These processors optimize the throughput of multiprogramming workloads rather than single-thread performance. We distinguish these processors from implicit multithreaded processors that utilize thread-level speculation by speculatively executing compiler- or machine-generated threads of control that are part of a single sequential program.This survey paper explains and classifies the explicit multithreading techniques in research and in commercial microprocessors.

Dynamic imaging of protease activity with fluorescently quenched activity-based probes

Abstract: Protease activity is tightly regulated in both normal and disease conditions. However, it is often difficult to monitor the dynamic nature of this regulation in the context of a live cell or whole organism. To address this limitation, we developed a series of quenched activity-based probes (qABPs) that become fluorescent upon activity-dependent covalent modification of a protease target. These reagents freely penetrate cells and allow direct imaging of protease activity in living cells. Targeted proteases are directly identified and monitored biochemically by virtue of the resulting covalent tag, thereby allowing unambiguous assignment of protease activities observed in imaging studies. We report here the design and synthesis of a selective, cell-permeable qABP for the study of papain-family cysteine proteases. This probe is used to monitor real-time protease activity in live human cells with fluorescence microscopy techniques as well as standard biochemical methods.