Keele University

About: Keele University is a education organization based out in Newcastle-under-Lyme, United Kingdom. It is known for research contribution in the topics: Population & Stars. The organization has 11318 authors who have published 26323 publications receiving 894671 citations. The organization is also known as: Keele University.

Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery.

Abstract: The recent emphasis on the development of non-viral transfection agents for gene delivery has led to new physics and chemistry-based techniques, which take advantage of charge interactions and energetic processes. One of these techniques which shows much promise for both in vitro and in vivo transfection involves the use of biocompatible magnetic nanoparticles for gene delivery. In these systems, therapeutic or reporter genes are attached to magnetic nanoparticles, which are then focused to the target site/cells via high-field/high-gradient magnets. The technique promotes rapid transfection and, as more recent work indicates, excellent overall transfection levels as well. The advantages and difficulties associated with magnetic nanoparticle-based transfection will be discussed as will the underlying physical principles, recent studies and potential future applications.

Using mapping studies as the basis for further research - A participant-observer case study

Abstract: Context: We are strong advocates of evidence-based software engineering (EBSE) in general and systematic literature reviews (SLRs) in particular. We believe it is essential that the SLR methodology is used constructively to support software engineering research. Objective: This study aims to assess the value of mapping studies which are a form of SLR that aims to identify and categorise the available research on a broad software engineering topic. Method: We used a multi-case, participant-observer case study using five examples of studies that were based on preceding mapping studies. We also validated our results by contacting two other researchers who had undertaken studies based on preceding mapping studies and by assessing review comments related to our follow-on studies. Results: Our original case study identified 11 unique benefits that can accrue from basing research on a preceding mapping study of which only two were case specific. We also identified nine problems associated with using preceding mapping studies of which two were case specific. These results were consistent with the information obtained from the validation activities. We did not find an example of an independent research group making use of a mapping study produced by other researchers. Conclusion: Mapping studies can save time and effort for researchers and provide baselines to assist new research efforts. However, they must be of high quality in terms of completeness and rigour if they are to be a reliable basis for follow-on research.

Evidence-based recommendations for the role of exercise in the management of osteoarthritis of the hip or knee—the MOVE consensus

Abstract: Objectives. Exercise is an effective and commonly prescribed intervention for lower limb osteoarthritis (OA). Many unanswered questions remain, however, concerning the practical delivery of exercise therapy. We have produced evidence-based recommendations to guide health-care practitioners. Methods. A multidisciplinary guideline development group was formed from representatives of professional bodies to which OA is of relevance and other interested parties. Each participant contributed up to 10 propositions describing key clinical points regarding exercise therapy for OA of the hip or knee. Ten final recommendations were agreed by the Delphi technique. The research evidence for each was determined. A literature search was undertaken in the Medline, PubMed, EMBASE, PEDro, CINAHL and Cochrane databases. The methodological quality of each retrieved publication was assessed. Outcome data were

EULAR evidence-based recommendations for the diagnosis of knee osteoarthritis

Abstract: Objective To develop evidence-based recommendations for the diagnosis of knee osteoarthritis (OA). Methods The multidisciplinary guideline development group, representing 12 European countries, generated 10 key propositions regarding diagnosis using a Delphi consensus approach. For each recommendation, research evidence was searched systematically. Whenever possible, the sensitivity, specificity and likelihood ratio were calculated for individual diagnostic indicators and a diagnostic ladder was developed using Bayes' method. Secondary analyses were undertaken to test directly the recommendations using multiple predictive models in two populations from the UK and the Netherlands. Strength of recommendation was assessed by the EULAR visual analogue scale. Results Recommendations covered the definition of knee OA and its risk factors, subsets, typical symptoms and signs, the use of imaging and laboratory tests and differential diagnosis. Three symptoms (persistent knee pain, limited morning stiffness and reduced function) and three signs (crepitus, restricted movement and bony enlargement) appeared to be the most useful. Assuming a 12.5% background prevalence of knee OA in adults aged >= 45 years, the estimated probability of having radiographic knee OA increased with increasing number of positive features, to 99% when all six symptoms and signs were present. The performance of the recommendations in the study populations varied according to the definition of knee OA, background risk and number of tests applied. Conclusion 10 key recommendations for diagnosis of knee OA were developed using both research evidence and expert consensus. Although there is no agreed reference standard, thorough clinical assessment alone can provide a confident rule-in diagnosis.