scispace - formally typeset
Search or ask a question
Institution

Kettering University

EducationFlint, Michigan, United States
About: Kettering University is a education organization based out in Flint, Michigan, United States. It is known for research contribution in the topics: RNA & Antigen. The organization has 6842 authors who have published 7689 publications receiving 337503 citations. The organization is also known as: GMI Engineering & Management Institute & General Motors Institute.
Topics: RNA, Antigen, DNA, Cancer, Population


Papers
More filters
Journal ArticleDOI
TL;DR: Saline suspensions of a variety of connective tissues and of collagen clump platelets in citrated platelet-rich plasma even in dilutions of 1:100, and the probable role of the connective tissue factor in hemostasis is discussed.
Abstract: SummarySaline suspensions of a variety of connective tissues and of collagen clump platelets in citrated platelet-rich plasma even in dilutions of 1:100. The effect is unrelated to blood clotting. The active material in human subcutaneous tissue is sedimented at 25,000 g in one hour, stable at 56°C, but not at 70°C, and destroyed by collagenase, form-amide and 25% alcohol but not by proteolytic enzymes. An inhibitor is present in liver. Similarities and differences between the effects of purified collagen, tissue suspensions and thrombin on platelets are described and the probable role of the connective tissue factor in hemostasis is discussed.

233 citations

Journal ArticleDOI
25 Nov 2020-Nature
TL;DR: Influence of the gut microbiome on the human immune system is revealed by systems analysis of vast clinical data from decades of electronic health records paired with massive longitudinal microbiome sequencing, with implications for microbiota-driven modulation of immunity.
Abstract: The gut microbiota influences development1–3 and homeostasis4–7 of the mammalian immune system, and is associated with human inflammatory8 and immune diseases9,10 as well as responses to immunotherapy11–14. Nevertheless, our understanding of how gut bacteria modulate the immune system remains limited, particularly in humans, where the difficulty of direct experimentation makes inference challenging. Here we study hundreds of hospitalized—and closely monitored—patients with cancer receiving haematopoietic cell transplantation as they recover from chemotherapy and stem-cell engraftment. This aggressive treatment causes large shifts in both circulatory immune cell and microbiota populations, enabling the relationships between the two to be studied simultaneously. Analysis of observed daily changes in circulating neutrophil, lymphocyte and monocyte counts and more than 10,000 longitudinal microbiota samples revealed consistent associations between gut bacteria and immune cell dynamics. High-resolution clinical metadata and Bayesian inference allowed us to compare the effects of bacterial genera in relation to those of immunomodulatory medications, revealing a considerable influence of the gut microbiota—together and over time—on systemic immune cell dynamics. Our analysis establishes and quantifies the link between the gut microbiota and the human immune system, with implications for microbiota-driven modulation of immunity. Influence of the gut microbiome on the human immune system is revealed by systems analysis of vast clinical data from decades of electronic health records paired with massive longitudinal microbiome sequencing.

233 citations

Journal ArticleDOI
TL;DR: This review article summarizes the historical development and various modes of the technique, some theoretical and practical aspects, recent trends and selected applications of single drop microextraction.

232 citations

Journal ArticleDOI
TL;DR: It is concluded that association of Kit with p85PI3‐K, and thus with PI3‐kinase activity, is necessary for a full mitogenic as well as adhesive response in mast cells.
Abstract: The pleiotropic effects of the Kit receptor system are mediated by Kit-Ligand (KL) induced receptor autophosphorylation and its association with and activation of distinct second messengers, including phosphatidylinositol 3'-kinase (PI3-kinase), p21ras and mitogen-activated protein kinase (MAPK) To define the role of PI3-kinase, p21ras and MAPK in Kit-mediated cell proliferation, survival and adhesion in bone marrow-derived mast cells (BMMC), mutant Kit receptors were expressed in Wsh/Wsh BMMC lacking endogenous c-kit expression The introduction of both murine Kit(S) and KitL (isoform containing a four amino acid insert) into Wsh/Wsh BMMC restored KL-induced proliferation, survival and adhesion to fibronectin, as well as activation of PI3-kinase, p21ras and MAPK, and induced expression of c-fos, junB, c-myc and c-myb mRNA Substitution of tyrosine 719 in the kinase insert with phenylalanine (Y719F) abolished PI3-kinase activation, diminished c-fos and junB induction, and impaired KL-induced adhesion of BMMC to fibronectin In addition, the Y719F mutation had partial effects on p21ras activation, cell proliferation and survival, while MAP kinase activation was not affected On the other hand, Y821F substitution impaired proliferation and survival without affecting PI3-kinase, p21ras and MAPK activation, and induction of c-myc, c-myb, c-fos and c-jun mRNA, while KL-induced cell adhesion to fibronectin remained intact In agreement with a role for PI3-kinase in Kit-mediated cell adhesion, wortmannin blocked Kit-mediated cell adhesion at concentrations known to specifically inhibit PI3-kinase We conclude, that association of Kit with p85PI3-K, and thus with PI3-kinase activity, is necessary for a full mitogenic as well as adhesive response in mast cells In contrast, tyrosine 821 is essential for Kit-mediated mitogenesis and survival, but not cell adhesion

232 citations

Journal ArticleDOI
TL;DR: This review will focus on recent advances in understanding of the structure, regulation, and functions of the effector kinases in the DDR, as well as their potential roles in human disease.

231 citations


Authors

Showing all 6853 results

NameH-indexPapersCitations
Joan Massagué189408149951
Chris Sander178713233287
Timothy A. Springer167669122421
Murray F. Brennan16192597087
Charles M. Rice15456183812
Lloyd J. Old152775101377
Howard I. Scher151944101737
Paul Tempst14830989225
Pier Paolo Pandolfi14652988334
Barton F. Haynes14491179014
Jedd D. Wolchok140713123336
James P. Allison13748383336
Harold E. Varmus13749676320
Scott W. Lowe13439689376
David S. Klimstra13356461682
Network Information
Related Institutions (5)
National Institutes of Health
297.8K papers, 21.3M citations

92% related

University of Minnesota
257.9K papers, 11.9M citations

91% related

Washington University in St. Louis
163.7K papers, 10M citations

91% related

University of Pittsburgh
201K papers, 9.6M citations

91% related

Duke University
200.3K papers, 10.7M citations

90% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20238
202216
2021211
2020234
2019204
2018225