Kettering University

About: Kettering University is a education organization based out in Flint, Michigan, United States. It is known for research contribution in the topics: RNA & Antigen. The organization has 6842 authors who have published 7689 publications receiving 337503 citations. The organization is also known as: GMI Engineering & Management Institute & General Motors Institute.

Cerebellum morphogenesis: the foliation pattern is orchestrated by multi-cellular anchoring centers

Abstract: Background The cerebellum has a striking morphology consisting of folia separated by fissures of different lengths. Since folia in mammals likely serve as a broad platform on which the anterior-posterior organization of the sensory-motor circuits of the cerebellum are built, it is important to understand how such complex morphology arises.

Nuclear positioning in muscle development and disease.

Abstract: Muscle disease as a group is characterized by muscle weakness, muscle loss, and impaired muscle function. Although the phenotype is the same, the underlying cellular pathologies, and the molecular causes of these pathologies, are diverse. One common feature of many muscle disorders is the mispositioning of myonuclei. In unaffected individuals, myonuclei are spaced throughout the periphery of the muscle fiber such that the distance between nuclei is maximized. However, in diseased muscles, the nuclei are often clustered within the center of the muscle cell. Although this phenotype has been acknowledged for several decades, it is often ignored as a contributor to muscle weakness. Rather, these nuclei are taken only as a sign of muscle repair. Here we review the evidence that mispositioned myonuclei are not merely a symptom of muscle disease but also a cause. Additionally, we review the working models for how myonuclei move from two different perspectives: from that of the nuclei and from that of the cytoskeleton. We further compare and contrast these mechanisms with the mechanisms of nuclear movement in other cell types both to draw general themes for nuclear movement and to identify muscle-specific considerations. Finally, we focus on factors that can be linked to muscle disease and find that genes that regulate myonuclear movement and positioning have been linked to muscular dystrophy. Although the cause-effect relationship is largely speculative, recent data indicate that the position of nuclei should no longer be considered only a means to diagnose muscle disease.

Total synthesis of taxol

Abstract: The present invention provides two basic routes for the total synthesis of taxol having the structure: ##STR1## The present invention also provides the intermediates produced in the above processes, processes for synthesizing these intermediates as well as analogs to taxol. Both the intermediates and analogs to taxol may prove to be valuable anticancer agents.

A new maraglas, d.e.r.® 732, embedment for electron microscopy

Abstract: A new epoxy embedding mixture for biological material recently has been introduced by Freeman and Spurlock (1). This mixture consists of Maraglas 655, Cardolite NC-513, dibutyl phthalate, and the curing agent benzyldimethylamine (BDMA). Maraglas has certain advantages over other resins and epoxies, notably, wide range of miscibility, low viscosity, ease of sectioning, good staining qualities, beam stability, and little background granularity. Although improvements have been made in the original technique (2), certain problems remain. The Maraglas mixture penetrates tissue very slowly, makes the tissue quite brittle, and has variable polymerization properties. In this paper the use and advantages of a new polyglycol type flexibilizer as a replacement for Cardolite NC-513 in a Maraglas 655 epoxy formulation will be presented.