King Faisal Specialist Hospital & Research Centre

About: King Faisal Specialist Hospital & Research Centre is a healthcare organization based out in Riyadh, Saudi Arabia. It is known for research contribution in the topics: Detection limit & High-performance liquid chromatography. The organization has 58 authors who have published 157 publications receiving 3527 citations.

Construction and characterization of potentiometric sensor for the determination of oxytetracycline hydrochloride.

Abstract: The construction and performance of plastic membrane (PME) oxytetracycline hydrochloride (OTC)-selective electrodes, based on three types of ion-pairs, OTC-tetraphenylborate (TPB), OTC-phosphotungstate (PT) and OTC-silicotungstate (SiT), as the electroactive substance in a plasticized PVC membrane with dibutylphthalate (DBP), were described. Furthermore, internal solid contact sensors (ISCS) OTC-selective sensors, based on a conducting polypyrrole (PPy) film immobilized on a platinum or glassy carbon electrode surface casted by a plasticized PVC membrane were constructed and evaluated. A novel optimization methodology in a numerical expression, comprehensive quality index (CQI) for a sensor, was used to optimize the composition of the membrane in the preparation of PME and screen the parameter of electropolymerization in the preparation of ISCS. Several PMEs and ISCSs were fully characterized and evaluated. An ISCS (Pt/PPy/PVC (OTC-TPB)) showed an excellent Nernstian response over the linear concentration range of 4.0 x 10(-7)-5.0 x 10(-2) M with the slope of 60.6 mV per decade (at 25 degrees C). The limit of detection was 1.0 x 10(-7) M of OTC (0.03 ppm). The response time was <25 s. The sensor was successfully used for the analysis of OTC in pharmaceutical formulation by using direct potentiometry.

High throughput analysis of lamivudine in pharmaceutical preparations using monolithic silica HPLC column

Abstract: A new, rapid and sensitive high performance liquid chromatography (HPLC) assay was developed and validated, which utilized a monolithic column (100 × 4.6 mm I.D.) for the determination of lamivudine in pharmaceutical preparations. The method used a mobile phase consisting of acetonitrile/water (65:35, v/v) and flow rate of 2.0 mL/min. The elution of the analyte was monitored at 285 nm and conducted at ambient temperature. The method was validated with respect to linearity range, limit of detection and quantitation, precision, accuracy, selectivity, and robustness. The method exhibited low limit of detection (LOD) of 12.5 ng/mL, wide linearity range of 25–800 ng/mL and correlation coefficient (R 2) greater than 0.999. Parameters of validation prove the precision of the method and its applicability for the determination of lamivudine in pharmaceutical tablet formulations. The method is fast (less than one minute) and is suitable for high throughput analysis of the drug.

Fast Screening of Phenol and Its Derivatives in Wastewater by HPLC by Using Monolithic Silica Column andSolid‐Phase Extraction

Abstract: A simple, fast, inexpensive, and reproducible high performance liquid chromatography and solid phase extraction (HPLC‐SPE) method was developed for the analysis of 4‐cyanophenol, phenol, 4‐nitrophenol, and 4‐chlorophenol in wastewater. The column used was a monolithic silica column, Chromolith Performance RP‐18e 100‐4,6 (100 × 4.6 mm). The mobile phase used was water–acetonitrile (80:20, v/v) at the flow rate ranging from 1 to 10 mL/min. The detection was achieved by using the ultraviolet (UV) mode at 246 nm. The detection limits of these phenolic compounds ranged from 1 to 5 ng. The separation of these phenolic compounds was achieved at a maximum time of 1.22 min by using 10 mL/min as the flow rate. The separation factors of all the studied phenols were in the range of 2.11 to 17.58, while the resolution factors were from 1.00 to 2.33.