Showing papers by "Korea Forest Service published in 2019"
Journal Article•
TL;DR: Cryopreservation of in vitro shoot tips using droplet-vitrification was developed as a complementary conservation approach for A. altaicus to complement traditional conservation approaches in case seeds are not available or insufficient for conservation programs.
Abstract: Background Aster altaicus var. uchiyamae Kitam is an endemic and endangered species in urgent need of a comprehensive conservation strategy. Objective To develop an efficient cryopreservation protocol using in vitro shoot tips to complement traditional conservation approaches in case seeds are not available or insufficient for conservation programs. Methods Shoot tips of in vitro plants were cryopreserved using a droplet-vitrification method following improvement of pre-culture, osmoprotection, vitrification solution (VS), unloading and post-culture treatments. The starting protocol included step-wise pre-culture with 10% and 17.5% sucrose for 55 h and 17 h, respectively, followed by osmoprotection with C4-35% (17.5% glycerol + 17.5% sucrose) for 30 min, and cryoprotection with B5-80% (40% glycerol + 40% sucrose) for 60 min. Results Shoot tips of A. altaicus were found to be moderately sensitive to the osmotic stress. Pre-culture and osmoprotection were not critical for the regeneration of cryopreserved explants when either of these treatments was applied. Osmoprotection with C4-35% on ice for 60 min followed by cryoprotection with A3-80%, a modified and diluted PVS2, on ice for 60 min resulted in the highest (65.3%) regeneration of cryopreserved shoot tips. Among alternative VSs tested, A3-80% and B5-80% were superior to PVS2 and PVS3 used under the same conditions. Step-wise recovery of shoot tips on ammonium-free medium followed by GA3-containing medium and medium without growth regulators were critical for the normal regeneration of both VS-treated and cryopreserved shoot tips. Conclusions Cryopreservation of in vitro shoot tips using droplet-vitrification was developed as a complementary conservation approach for A. altaicus. Adjustment of the composition of regrowth media depending on recovery stage was important for the regeneration of healthy plants from cryopreserved shoot tips.
8 citations
TL;DR: In this article, the potential of Fourier transform infrared (FT-NIR) spectroscopy in determining the viable seeds of Hinoki cypress was investigated, and a multivariate classification with partial least square discriminant analysis (PLS-DA) was developed to differentiate between viable and nonviable seeds.
8 citations
TL;DR: In this paper, the authors measured tree ages in four Korean village groves (KVGs) and one PF in Korea and found that KVG trees were statistically younger than those of the PF (Seorak Forest), indicating that significant age gaps exist between the actual tree ages and the historical ages of KVGs.
5 citations
TL;DR: This study developed a simple volume model for Cryptomeria japonica found in Jeju Island, South Korea using the diameter at breast height (DBH) and the total height of a tree as variables to evaluate the performance of six models.
Abstract: The accurate estimation of the stem volume of trees is an important task for forest managers. Two common variables used in stem volume models are the diameter at breast height (DBH) and the total h...
4 citations
TL;DR: Artemisia sieversiana seeds can be safely preserved without deterioration of vitality at cryopreservation by using PVS 3 solution and it will be helpful in future studies.
Abstract: Artemisia sieversiana is being used for the medicinal purpose, there are not many conservation objects, and the species is registered as a rare plant in Korea. There is also no research on ...
1 citations
TL;DR: The maximum likelihood phylogenetic analysis revealed that C. coreana was closely related to Sinowilsonia, and the total length was 159,398 bp, containing a pair of 26,270 bp inverted repeat region.
Abstract: The complete chloroplast genome sequence of Corylopsis coreana, the first sequenced of the genus Corylopsis, was reported in this study. The total length was 159,398 bp, containing a pair of 26,270 bp inverted repeat region, which were separated by SSC region and LSC region of 18,692 and 88,163 bp, respectively. The overall GC content of the genome was 38%. The genome encoded 130 genes, including 85 protein-coding genes, 37 transfer RNA genes, and eight ribosomal RNA genes. The maximum likelihood phylogenetic analysis revealed that C. coreana was closely related to Sinowilsonia.
1 citations
TL;DR: The authors wish to add the following corrections to their paper published in the International Journal of Environmental Research and Public Health:.
Abstract: The authors wish to add the following corrections to their paper published in the International Journal of Environmental Research and Public Health [...]