Kuvempu University

About: Kuvempu University is a education organization based out in Shimoga, India. It is known for research contribution in the topics: Cyclic voltammetry & Carbon paste electrode. The organization has 1575 authors who have published 2210 publications receiving 39755 citations. The organization is also known as: KU.

Phytosynthesis of gold nanoparticles using Mappia foetida leaves extract and their conjugation with folic acid for delivery of doxorubicin to cancer cells.

Abstract: Mappia foetida leaves extract is used as bioreductant for the synthesis of gold nanoparticles and their application in the efficient delivery of doxorubicin to human cancer cells is reported here. The formation of gold nanoparticles is evident from their characteristic optical absorption at *560 nm. X-ray diffraction pattern of gold nanoparticles confirmed their fcc structure. Fourier trans- form infrared spectroscopy shows the bioactive molecules from plant extract capped on the surface of gold nanopar- ticles and conjugation of doxorubicin along with activated folic acid as navigational molecules for targeted drug delivery. Such a conjugation of gold nanoparticles is characterized by their weight loss, *35-40 %, due to thermal degradation of plant biomass and conjugated drug along with receptor, as observed in thermogravimetric analysis. The spherical shaped gold nanoparticles (U 10-20 nm) are observed by field emission scanning elec- tron microscopy and transmission electron microscopy images and the expected elemental composition by energy dispersive X-ray spectroscopy. Gold nanoparticles conju- gated with activated folic acid and doxorubicin complex is found to be toxic for human cancer cells viz., MDA-MB- 231, HeLa, SiHa and Hep-G2. Furthermore, the amount of drug released was maximum at pH 5.3 (an ambient con- dition for intravenous cancer drugs) followed by pH 7.2 and pH 6.8.

Pyrazole-based azo-metal(II) complexes as potential bioactive agents: synthesis, characterization, antimicrobial, anti-tuberculosis, and DNA interaction studies

Abstract: In the present investigation, the bioactive azo-dye ligand 1,5-dimethyl-4-[(3-methyl-5-oxo-1-phenyl-4,5-dihydro-1H-pyrazol-4-yl)diazenyl]-2-phenyl-1,2-dihydro-3H-pyrazol-3-one (L) and its t...

Paclobutrazol induced changes in carbohydrates and some associated enzymes during floral initiation in mango (Mangifera indica L.) cv. Totapuri

Abstract: In order to study the role of carbohydrates in the paclobutrazol induced floral initiation in mango (Mangifera indica L.), the carbohydrate contents and the activities of amylase and sucrose metabolizing enzymes were analyzed in the apical buds and leaves of growing shoots at 4 bud developmental stages numerically characterized as 510 (initiation of bud swelling), 511 (swollen buds), 513 (bud burst) and 515 (panicle emergence) according to standard BBCH scale. The soil drenching treatment of paclobutrazol @ 3.0 ml m−1 canopy diameter to the mango cv. Totapuri resulted in increase in the contents of total soluble sugars, reducing sugars, starch, sucrose, glucose and fructose in the apical buds and leaves, and the increases were greater in apical buds. Both in the paclobutrazol treated and control trees, the contents of total soluble sugar, reducing sugar, sucrose, glucose and fructose showed a progressive increase from 510 to 513 bud developmental stages and declined subsequently. In contrast, leaf starch showed a consistent declining pattern from higher value at 510–515 bud developmental stages. The amylase activity in leaves declined, while the activities of acid invertase (AI), sucrose synthase (SS) and sucrose phosphate synthase (SPS) increased significantly in the apical buds of paclobutrazol treated trees as compared to control, with peak activity witnessed at 513 bud developmental stage. The results indicated that paclobutrazol induced flowering is accompanied by an increase in starch in leaf concomitant with an increase in soluble sugars like sucrose, glucose and fructose in apical buds and inhibition in the amylase activity in association with increase in the activities of AI, SPS and SS in the apical buds.