Kuvempu University

About: Kuvempu University is a education organization based out in Shimoga, India. It is known for research contribution in the topics: Cyclic voltammetry & Carbon paste electrode. The organization has 1575 authors who have published 2210 publications receiving 39755 citations. The organization is also known as: KU.

Isolation, characterization, antibacterial, antihelminthic, and in silico studies of polyprenol from Kirganelia reticulata Baill

Abstract: Microbial and helminthes infections are the most common health problems in India; in developing countries they pose a large treat to public. These infections can affect most population in endemic areas with major economic and social consequences. In vitro antibacterial activity of a phytoconstituent, polyprenol isolated from the leaves of Kirganelia reticulata was screened against Gram-negative and Gram-positive bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) of the purified antibacterial agent were also determined. The present study also undertook evaluation of antihelminthic activity of polyprenol at different concentrations using Pheretima posthuma as a test worm. The bioassay involved the determination of paralysis time and death time of the worm. The polyprenol showed antibacterial activity against different bacterial strains with MIC 6.25–25.0 mgml−1 and MBC 12.50–25.0 mgml−1, and possesses better glucosamine-6-phosphate synthase inhibition in molecular docking studies with minimum docking energy and better ligand efficiency when compared to the standard. The antihelminthic results of the present study indicated that polyprenol significantly demonstrated paralysis and caused death of worm in dose-dependent manner; showing comparable results with the standard in docking process. Hence, the isolated compound polyprenol is suggestive of being a good antibacterial and antihelminthic agent.

Antiproliferative, DNA cleavage, and ADMET study of substituted 2-(1-benzofuran-2-yl) quinoline-4-carboxylic acid and its esters

Abstract: Synthesis, anti-proliferative, DNA cleavage, and in silico ADMET studies of 2-(1-benzofuran-2-yl) quinoline-4-carboxylic acids and their resultant esters in acid catalyzed medium have been investigated. The synthesized compounds are characterized by UV, IR, 1H NMR, 13C NMR, and mass spectral analysis. The electrophoretic DNA cleavage studies on λ-DNA (Eco-RI/Hinda-III double digest) using agarose gel method and the antiproliferative activity was carried out by MTT assay on five different human cancer cell lines (Chronic Myelogenous Leukemia (K562), Breast Cancer (MCF-7), Cervical Cancer (HeLa), Colorectal Adino carcinoma (Colo 205), and Hepato cellular carcinoma (HepG2)). Doxorubicin is taken as standard for comparison. The cleavage study indicated that molecules (3b–6a and 7b–8c) did cleave the DNA completely with no trace of fragments. The molecules (6b, 6c and 7a) have appeared to cleave DNA partially and assessed by comparing the bands appeared in control and test compounds at 100 μg concentra...

2-Thieno/Selenopyrano[2,3-b]quinolines: Microwave-Induced One-Pot Synthesis, DNA Binding, and Photocleavage Studies

Abstract: We have characterized a new class of 2-thieno/2-selenopyrano[2,3-b]quinolin-2-ol (2c/3c) intercalators exhibiting a novel type of DNA binding and photocleavage activity. The dynamic behavior of these compounds with DNA was investigated by absorption spectra (obtained Kb constant for 2c is 3.6 × 106 and for 3c is 2.8 × 105), viscosity, and thermal denaturation studies. Intrinsic binding constants (Kb) have been estimated under a similar set of experimental conditions. An absorption spectral study shows the strong interaction between synthesized 2c, 3c, and base pairs CT-DNA. In addition, the effects of these compounds on DNA cleavage were investigated by photoirradiation at 360 nm and show that the hybrids effectively cleaved double-stranded DNA with UV light of a long wavelength and without any additive.