scispace - formally typeset
Search or ask a question
Institution

Kyoto University

EducationKyoto, Japan
About: Kyoto University is a education organization based out in Kyoto, Japan. It is known for research contribution in the topics: Population & Catalysis. The organization has 85837 authors who have published 217215 publications receiving 6526826 citations. The organization is also known as: Kyōto University & Kyōto daigaku.


Papers
More filters
Journal ArticleDOI
TL;DR: It is concluded that in nonepithelial cells, ZO-1 works as a cross-linker between cadherin/catenin complex and the actin-based cytoskeleton through direct interaction with α catenin and actin filaments at its amino- and carboxyl-terminal halves, respectively, and that Z O-1 is a functional component in the cadher in-based cell adhesion system.
Abstract: ZO-1, a 220-kD peripheral membrane protein consisting of an amino-terminal half discs large (dlg)-like domain and a carboxyl-terminal half domain, is concentrated at the cadherin-based cell adhesion sites in non-epithelial cells. We introduced cDNAs encoding the full-length ZO-1, its amino-terminal half (N-ZO-1), and carboxyl-terminal half (C-ZO-1) into mouse L fibroblasts expressing exogenous E-cadherin (EL cells). The full-length ZO-1 as well as N-ZO-1 were concentrated at cadherin-based cell–cell adhesion sites. In good agreement with these observations, N-ZO-1 was specifically coimmunoprecipitated from EL transfectants expressing N-ZO-1 (NZ-EL cells) with the E-cadherin/α, β catenin complex. In contrast, C-ZO-1 was localized along actin stress fibers. To examine the molecular basis of the behavior of these truncated ZO-1 molecules, N-ZO-1 and C-ZO-1 were produced in insect Sf9 cells by recombinant baculovirus infection, and their direct binding ability to the cadherin/catenin complex and the actin-based cytoskeleton, respectively, were examined in vitro. Recombinant N-ZO-1 bound directly to the glutathione-S-transferase fusion protein with α catenin, but not to that with β catenin or the cytoplasmic domain of E-cadherin. The dissociation constant between N-ZO-1 and α catenin was ∼0.5 nM. On the other hand, recombinant C-ZO-1 was specifically cosedimented with actin filaments in vitro with a dissociation constant of ∼10 nM. Finally, we compared the cadherin-based cell adhesion activity of NZ-EL cells with that of parent EL cells. Cell aggregation assay revealed no significant differences among these cells, but the cadherin-dependent intercellular motility, i.e., the cell movement in a confluent monolayer, was significantly suppressed in NZ-EL cells. We conclude that in nonepithelial cells, ZO-1 works as a cross-linker between cadherin/catenin complex and the actin-based cytoskeleton through direct interaction with α catenin and actin filaments at its amino- and carboxyl-terminal halves, respectively, and that ZO-1 is a functional component in the cadherin-based cell adhesion system.

674 citations

Journal ArticleDOI
TL;DR: Enantioselective reactions catalyzed by urea and thiourea derivatives as general acid catalysts as well as diastereoselectives reactions are described.
Abstract: Hydrogen-bonding interaction plays a crucial role in the molecular recognition and activation processes of various biologically important reactions that are mediated by enzymes and antibodies in living organisms. Recently, it has been shown that a hydrogen-bonding donor can be used as a general acid catalyst for various types of reactions in organic chemistry. In this article, we describe enantioselective reactions catalyzed by urea and thiourea derivatives as general acid catalysts as well as diastereoselective reactions. This perspective provides an overview of this rapidly growing field.

673 citations

Journal ArticleDOI
Emek Demir1, Emek Demir2, Michael P. Cary2, Suzanne M. Paley3, Ken Fukuda, Christian Lemer4, Imre Vastrik, Guanming Wu5, Peter D'Eustachio6, Carl F. Schaefer7, Joanne S. Luciano, Frank Schacherer, Irma Martínez-Flores8, Zhenjun Hu9, Verónica Jiménez-Jacinto8, Geeta Joshi-Tope10, Kumaran Kandasamy11, Alejandra López-Fuentes8, Huaiyu Mi3, Elgar Pichler, Igor Rodchenkov12, Andrea Splendiani13, Andrea Splendiani14, Sasha Tkachev15, Jeremy Zucker16, Gopal R. Gopinath17, Harsha Rajasimha7, Harsha Rajasimha18, Ranjani Ramakrishnan19, Imran Shah20, Mustafa H Syed21, Nadia Anwar2, Özgün Babur2, Özgün Babur1, Michael L. Blinov22, Erik Brauner23, Dan Corwin, Sylva L. Donaldson12, Frank Gibbons23, Robert N. Goldberg24, Peter Hornbeck15, Augustin Luna7, Peter Murray-Rust25, Eric K. Neumann, Oliver Reubenacker22, Matthias Samwald26, Matthias Samwald27, Martijn P. van Iersel28, Sarala M. Wimalaratne29, Keith Allen30, Burk Braun, Michelle Whirl-Carrillo31, Kei-Hoi Cheung32, Kam D. Dahlquist33, Andrew Finney, Marc Gillespie34, Elizabeth M. Glass21, Li Gong31, Robin Haw5, Michael Honig35, Olivier Hubaut4, David W. Kane36, Shiva Krupa37, Martina Kutmon38, Julie Leonard30, Debbie Marks23, David Merberg39, Victoria Petri40, Alexander R. Pico41, Dean Ravenscroft42, Liya Ren10, Nigam H. Shah31, Margot Sunshine7, Rebecca Tang30, Ryan Whaley30, Stan Letovksy43, Kenneth H. Buetow7, Andrey Rzhetsky44, Vincent Schächter45, Bruno S. Sobral18, Ugur Dogrusoz1, Shannon K. McWeeney19, Mirit I. Aladjem7, Ewan Birney, Julio Collado-Vides8, Susumu Goto46, Michael Hucka47, Nicolas Le Novère, Natalia Maltsev21, Akhilesh Pandey11, Paul Thomas3, Edgar Wingender, Peter D. Karp3, Chris Sander2, Gary D. Bader12 
TL;DR: Thousands of interactions, organized into thousands of pathways, from many organisms are available from a growing number of databases, and this large amount of pathway data in a computable form will support visualization, analysis and biological discovery.
Abstract: Biological Pathway Exchange (BioPAX) is a standard language to represent biological pathways at the molecular and cellular level and to facilitate the exchange of pathway data. The rapid growth of the volume of pathway data has spurred the development of databases and computational tools to aid interpretation; however, use of these data is hampered by the current fragmentation of pathway information across many databases with incompatible formats. BioPAX, which was created through a community process, solves this problem by making pathway data substantially easier to collect, index, interpret and share. BioPAX can represent metabolic and signaling pathways, molecular and genetic interactions and gene regulation networks. Using BioPAX, millions of interactions, organized into thousands of pathways, from many organisms are available from a growing number of databases. This large amount of pathway data in a computable form will support visualization, analysis and biological discovery.

673 citations

Journal ArticleDOI
Tohru Saeki1, Kazumitsu Ueda1, Yusuke Tanigawara, Ryohei Hori1, Tohru Komano1 
TL;DR: Results indicate that P-glycoprotein transports the immunosuppressive agents cyclosporin A and FK506.

672 citations

Journal ArticleDOI
M. H. Ahn1, E. Aliu2, S. Andringa2, Shigeki Aoki3  +217 moreInstitutions (29)
TL;DR: In this article, measurements of {nu}{sub {mu}} disappearance in K2K, the KEK to Kamioka long-baseline neutrino oscillation experiment are presented.
Abstract: We present measurements of {nu}{sub {mu}} disappearance in K2K, the KEK to Kamioka long-baseline neutrino oscillation experiment. One-hundred and twelve beam-originated neutrino events are observed in the fiducial volume of Super-Kamiokande with an expectation of 158.1{sub -8.6}{sup +9.2} events without oscillation. A distortion of the energy spectrum is also seen in 58 single-ring muonlike events with reconstructed energies. The probability that the observations are explained by the expectation for no neutrino oscillation is 0.0015% (4.3{sigma}). In a two-flavor oscillation scenario, the allowed {delta}m{sup 2} region at sin{sup 2}2{theta}=1 is between 1.9 and 3.5x10{sup -3} eV{sup 2} at the 90% C.L. with a best-fit value of 2.8x10{sup -3} eV{sup 2}.

672 citations


Authors

Showing all 86225 results

NameH-indexPapersCitations
Kari Alitalo174817114231
Ralph M. Steinman171453121518
Masayuki Yamamoto1711576123028
Karl Deisseroth160556101487
Kenji Kangawa1531117110059
Takashi Taniguchi1522141110658
Ben Zhong Tang1492007116294
Takeo Kanade147799103237
Yuji Matsuzawa143836116711
Tasuku Honjo14171288428
Kenneth M. Yamada13944672136
Y. B. Hsiung138125894278
Shuh Narumiya13759570183
Kevin P. Campbell13752160854
Junji Tojo13587884615
Network Information
Related Institutions (5)
University of Tokyo
337.5K papers, 10.1M citations

99% related

Nagoya University
128.2K papers, 3.2M citations

99% related

Osaka University
185.6K papers, 5.1M citations

97% related

University of Tsukuba
79.4K papers, 1.9M citations

97% related

Hokkaido University
115.4K papers, 2.6M citations

97% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
2023234
2022679
20218,533
20208,740
20198,050
20187,932