Showing papers by "Kyungpook National University published in 2017"
••
University of Ulsan1, Osaka University2, Taipei Veterans General Hospital3, University Health System4, Chang Gung University5, Samsung Medical Center6, National Taiwan University7, Korea University8, China Medical University (Taiwan)9, Kindai University10, Seoul National University Bundang Hospital11, Keio University12, Kyungpook National University13, Japanese Foundation for Cancer Research14, Seoul National University Hospital15, Yonsei University16, National Cheng Kung University17, National Health Research Institutes18
TL;DR: In this phase 3 study, the survival benefits indicate that nivolumab might be a new treatment option for heavily pretreated patients with advanced gastric or gastro-oesophageal junction cancer.
1,512 citations
••
TL;DR: A fully-fledged particle-flow reconstruction algorithm tuned to the CMS detector was developed and has been consistently used in physics analyses for the first time at a hadron collider as mentioned in this paper.
Abstract: The CMS apparatus was identified, a few years before the start of the LHC operation at CERN, to feature properties well suited to particle-flow (PF) reconstruction: a highly-segmented tracker, a fine-grained electromagnetic calorimeter, a hermetic hadron calorimeter, a strong magnetic field, and an excellent muon spectrometer. A fully-fledged PF reconstruction algorithm tuned to the CMS detector was therefore developed and has been consistently used in physics analyses for the first time at a hadron collider. For each collision, the comprehensive list of final-state particles identified and reconstructed by the algorithm provides a global event description that leads to unprecedented CMS performance for jet and hadronic τ decay reconstruction, missing transverse momentum determination, and electron and muon identification. This approach also allows particles from pileup interactions to be identified and enables efficient pileup mitigation methods. The data collected by CMS at a centre-of-mass energy of 8\TeV show excellent agreement with the simulation and confirm the superior PF performance at least up to an average of 20 pileup interactions.
719 citations
••
TL;DR: A multifunctional contact lens sensor that can measure the glucose level in tear fluid and intraocular pressure simultaneously but yet independently based on different electrical responses is developed.
Abstract: Wearable contact lenses which can monitor physiological parameters have attracted substantial interests due to the capability of direct detection of biomarkers contained in body fluids. However, previously reported contact lens sensors can only monitor a single analyte at a time. Furthermore, such ocular contact lenses generally obstruct the field of vision of the subject. Here, we developed a multifunctional contact lens sensor that alleviates some of these limitations since it was developed on an actual ocular contact lens. It was also designed to monitor glucose within tears, as well as intraocular pressure using the resistance and capacitance of the electronic device. Furthermore, in-vivo and in-vitro tests using a live rabbit and bovine eyeball demonstrated its reliable operation. Our developed contact lens sensor can measure the glucose level in tear fluid and intraocular pressure simultaneously but yet independently based on different electrical responses.
614 citations
••
U. Bhawandeep1, Vardan Khachatryan, Albert M. Sirunyan, Armen Tumasyan +2289 more•Institutions (147)
TL;DR: In this paper, the trigger system consists of two levels designed to select events of potential physics interest from a GHz (MHz) interaction rate of proton-proton (heavy ion) collisions.
Abstract: This paper describes the CMS trigger system and its performance during Run 1 of the LHC. The trigger system consists of two levels designed to select events of potential physics interest from a GHz (MHz) interaction rate of proton-proton (heavy ion) collisions. The first level of the trigger is implemented in hardware, and selects events containing detector signals consistent with an electron, photon, muon, tau lepton, jet, or missing transverse energy. A programmable menu of up to 128 object-based algorithms is used to select events for subsequent processing. The trigger thresholds are adjusted to the LHC instantaneous luminosity during data taking in order to restrict the output rate to 100 kHz, the upper limit imposed by the CMS readout electronics. The second level, implemented in software, further refines the purity of the output stream, selecting an average rate of 400 Hz for offline event storage. The objectives, strategy and performance of the trigger system during the LHC Run 1 are described.
532 citations
••
TL;DR: In this paper, an improved jet energy scale corrections, based on a data sample corresponding to an integrated luminosity of 19.7 fb^(-1) collected by the CMS experiment in proton-proton collisions at a center-of-mass energy of 8 TeV, are presented.
Abstract: Improved jet energy scale corrections, based on a data sample corresponding to an integrated luminosity of 19.7 fb^(-1) collected by the CMS experiment in proton-proton collisions at a center-of-mass energy of 8 TeV, are presented. The corrections as a function of pseudorapidity η and transverse momentum p_T are extracted from data and simulated events combining several channels and methods. They account successively for the effects of pileup, uniformity of the detector response, and residual data-simulation jet energy scale differences. Further corrections, depending on the jet flavor and distance parameter (jet size) R, are also presented. The jet energy resolution is measured in data and simulated events and is studied as a function of pileup, jet size, and jet flavor. Typical jet energy resolutions at the central rapidities are 15–20% at 30 GeV, about 10% at 100 GeV, and 5% at 1 TeV. The studies exploit events with dijet topology, as well as photon+jet, Z+jet and multijet events. Several new techniques are used to account for the various sources of jet energy scale corrections, and a full set of uncertainties, and their correlations, are provided. The final uncertainties on the jet energy scale are below 3% across the phase space considered by most analyses (p_T > 30 GeV and 0|η| 30 GeV is reached, when excluding the jet flavor uncertainties, which are provided separately for different jet flavors. A new benchmark for jet energy scale determination at hadron colliders is achieved with 0.32% uncertainty for jets with p_T of the order of 165–330 GeV, and |η| < 0.8.
505 citations
••
VU University Amsterdam1, King Abdullah University of Science and Technology2, Karolinska University Hospital3, Charité4, National University of Singapore5, National Institutes of Health6, University of Edinburgh7, ETH Zurich8, Kazan Federal University9, University Hospital Regensburg10, University of British Columbia11, Wistar Institute12, German Center for Neurodegenerative Diseases13, University of Queensland14, University of Melbourne15, Walter and Eliza Hall Institute of Medical Research16, University of Tokyo17, Harry Perkins Institute of Medical Research18, University of Western Australia19, Kyungpook National University20, Russian Academy of Sciences21, Engelhardt Institute of Molecular Biology22, Moscow Institute of Physics and Technology23, Lawrence Berkeley National Laboratory24, Ohu University25, Osaka University26, Lund University27, Norwegian University of Science and Technology28, Tokyo University of Pharmacy and Life Sciences29, University of Copenhagen30, Nihon University31, Memorial Sloan Kettering Cancer Center32
TL;DR: An integrated expression atlas of miRNAs and their promoters by deep-sequencing 492 short RNA libraries, with matching Cap Analysis Gene Expression (CAGE) data, is created, establishing a foundation for detailed analysis of miRNA expression patterns and transcriptional control regions.
Abstract: MicroRNAs (miRNAs) are short non-coding RNAs with key roles in cellular regulation. As part of the fifth edition of the Functional Annotation of Mammalian Genome (FANTOM5) project, we created an integrated expression atlas of miRNAs and their promoters by deep-sequencing 492 short RNA (sRNA) libraries, with matching Cap Analysis Gene Expression (CAGE) data, from 396 human and 47 mouse RNA samples. Promoters were identified for 1,357 human and 804 mouse miRNAs and showed strong sequence conservation between species. We also found that primary and mature miRNA expression levels were correlated, allowing us to use the primary miRNA measurements as a proxy for mature miRNA levels in a total of 1,829 human and 1,029 mouse CAGE libraries. We thus provide a broad atlas of miRNA expression and promoters in primary mammalian cells, establishing a foundation for detailed analysis of miRNA expression patterns and transcriptional control regions.
406 citations
••
University of California, Irvine1, University of Pennsylvania2, New York University3, University of Texas Southwestern Medical Center4, Kyungpook National University5, University of Central Florida6, Max Planck Society7, University of Strasbourg8, University of Southern California9, University of California, San Diego10
TL;DR: The myofibroblast is identified as a plastic cell type that may be manipulated to treat scars in humans through the bone morphogenetic protein signaling pathway and adipocyte transcription factors expressed during development.
Abstract: Although regeneration through the reprogramming of one cell lineage to another occurs in fish and amphibians, it has not been observed in mammals. We discovered in the mouse that during wound healing, adipocytes regenerate from myofibroblasts, a cell type thought to be differentiated and nonadipogenic. Myofibroblast reprogramming required neogenic hair follicles, which triggered bone morphogenetic protein (BMP) signaling and then activation of adipocyte transcription factors expressed during development. Overexpression of the BMP antagonist Noggin in hair follicles or deletion of the BMP receptor in myofibroblasts prevented adipocyte formation. Adipocytes formed from human keloid fibroblasts either when treated with BMP or when placed with human hair follicles in vitro. Thus, we identify the myofibroblast as a plastic cell type that may be manipulated to treat scars in humans.
385 citations
••
TL;DR: The first measurement of the tau lepton polarization P-tau(D*) in the decay (B) over bar -> D* tau(-) (v) over b (tau) as well as a new measurement of the ratio of the branching fractions was reported in this paper.
Abstract: We report the first measurement of the tau lepton polarization P-tau(D*) in the decay (B) over bar -> D* tau(-) (v) over bar (tau) as well as a newmeasurement of the ratio of the branching fractions R(D*) = B((B) over bar -> D* tau(-) (v) over bar (tau)) / B((B) over bar -> D* l(-) (v) over bar (l)), where l(-) denotes an electron or a muon, and the tau is reconstructed in the modes tau(-) -> pi(-) v(tau) and tau(-) -> rho(-) v(tau). We use the full data sample of 772 x 10(6) B (B) over bar pairs recorded with the Belle detector at the (KEKB) over bar electron-positron collider. Our results, P-tau(D*) = -0.38 +/- 0.51 (stat)(-0.16)(+0.21) (syst) and R(D*) = 0.270 +/- 0.035 (stat)(- 0.025)(+0.028) (syst), are consistent with the theoretical predictions of the standard model.
374 citations
••
TL;DR: In this paper, a review of hydrogen bonding interactions between metal-organic frameworks and adsorbates is presented, which will motivate the development of methods and techniques to utilize hydrogen bonding in several important adsorption-based applications of MOFs.
364 citations
••
Utrecht University1, United States Department of Energy2, National Autonomous University of Mexico3, National and Kapodistrian University of Athens4, University of Debrecen5, Leiden University6, Commonwealth Scientific and Industrial Research Organisation7, University of Göttingen8, Maynooth University9, University of Seville10, University of Natural Resources and Life Sciences, Vienna11, Broad Institute12, Huazhong Agricultural University13, State University of Campinas14, International Sleep Products Association15, Université libre de Bruxelles16, University of Nottingham17, Aix-Marseille University18, Pasteur Institute19, King Abdulaziz University20, Institut national de la recherche agronomique21, University of Helsinki22, University of Amsterdam23, University of St Andrews24, University of Ioannina25, Saint Petersburg State University26, Spanish National Research Council27, Federal University of São Carlos28, Swedish University of Agricultural Sciences29, Technical University of Berlin30, National Scientific and Technical Research Council31, Concordia University32, Kyungpook National University33, University of the Republic34, Karlsruhe Institute of Technology35, University of Sulaymaniyah36, University of Ljubljana37, Leibniz Association38, Stanford University39, Kansas State University40, Technical University of Denmark41, University of Wisconsin-Madison42, Pacific Northwest National Laboratory43, University of Jena44, University of São Paulo45, University of Kansas46, Université Paris-Saclay47, Imperial College London48, Vienna University of Technology49
TL;DR: In this article, a comparative genomics and experimental study of the aspergilli genus is presented, which allows for the first time a genus-wide view of the biological diversity of the Aspergillus and in many, but not all, cases linked genome differences to phenotype.
Abstract: Background: The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus. Results: We have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli. Conclusions: Many aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.
349 citations
••
TL;DR: The result is consistent with standard model (SM) expectations, where the largest discrepancy from a SM prediction is observed in the muon modes with a local significance of 2.6σ.
Abstract: We present a measurement of angular observables and a test of lepton flavor universality in the B -> K(+)l(+)l(-) decay, where l is either e or mu. The analysis is performed on a data sample corresponding to an integrated luminosity of 711 fb(-1) containing 772 x 10(6) B (B) over bar pairs, collected at the Upsilon(4S) resonance with the Belle detector at the asymmetric-energy e(+)e(-) collider KEKB. The result is consistent with standard model (SM) expectations, where the largest discrepancy from a SM prediction is observed in the muon modes with a local significance of 2.6 sigma.
••
TL;DR: The shape of the antineutrino energy spectrum obtained from the eight-month data-taking period is compared with a hypothesis of oscillations due to active-sterile antineUTrino mixing and no strong evidence of 3+1 neutrino oscillation is found.
Abstract: An experiment to search for light sterile neutrinos is conducted at a reactor with a thermal power of 2.8 GW located at the Hanbit nuclear power complex. The search is done with a detector consisting of a ton of Gd-loaded liquid scintillator in a tendon gallery approximately 24 m from the reactor core. The measured antineutrino event rate is 1976 per day with a signal to background ratio of about 22. The shape of the antineutrino energy spectrum obtained from the eight-month data-taking period is compared with a hypothesis of oscillations due to active-sterile antineutrino mixing. No strong evidence of $3+1$ neutrino oscillation is found. An excess around the 5 MeV prompt energy range is observed as seen in existing longer-baseline experiments. The mixing parameter ${\mathrm{sin}}^{2}2{\ensuremath{\theta}}_{14}$ is limited up to less than 0.1 for $\mathrm{\ensuremath{\Delta}}{m}_{41}^{2}$ ranging from 0.2 to $2.3\text{ }{\mathrm{eV}}^{2}$ with a 90% confidence level.
••
TL;DR: A new signature-based authenticated key establishment scheme for the IoT environment that provides more functionality features, and its computational and communication costs are also comparable with other existing approaches.
Abstract: Internet of Things (IoT) is a network of all devices that can be accessed through the Internet. These devices can be remotely accessed and controlled using existing network infrastructure, thus allowing a direct integration of computing systems with the physical world. This also reduces human involvement along with improving accuracy and efficiency, resulting in economic benefit. The devices in IoT facilitate the day-to-day life of people. However, the IoT has an enormous threat to security and privacy due to its heterogeneous and dynamic nature. Authentication is one of the most challenging security requirements in the IoT environment, where a user (external party) can directly access information from the devices, provided the mutual authentication between user and devices happens. In this paper, we present a new signature-based authenticated key establishment scheme for the IoT environment. The proposed scheme is tested for security with the help of the widely used Burrows-Abadi–Needham logic, informal security analysis, and also the formal security verification using the broadly accepted automated validation of Internet security protocols and applications tool. The proposed scheme is also implemented using the widely accepted NS2 simulator, and the simulation results demonstrate the practicability of the scheme. Finally, the proposed scheme provides more functionality features, and its computational and communication costs are also comparable with other existing approaches.
••
TL;DR: The exogenous application of Si has been found to induce stress tolerance by regulating the generation of ROS, reducing electrolytic leakage, and malondialdehyde (MDA) contents, and immobilizing and reducing the uptake of toxic ions like Na, under stressful conditions.
Abstract: Silicon (Si) is the second most abundant element in soil, where its availability to plants can exhilarate to 10% of total dry weight of the plant. Si accumulation/transport occurs in the upward direction, and has been identified in several crop plants. Si application has been known to ameliorate plant growth and development during normal and stressful conditions over past two-decades. During abiotic (salinity, drought, thermal, and heavy metal etc) stress, one of the immediate responses by plant is the generation of reactive oxygen species (ROS), such as singlet oxygen (1O2), superoxide (O2−), hydrogen peroxide (H2O2), and hydroxyl radicals (OH), which cause severe damage to the cell structure, organelles, and functions. To alleviate and repair this damage, plants have developed a complex antioxidant system to maintain homeostasis through non-enzymatic (carotenoids, tocopherols, ascorbate, and glutathione) and enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX)]. To this end, the exogenous application of Si has been found to induce stress tolerance by regulating the generation of ROS, reducing electrolytic leakage and malondialdehyde (MDA) contents, and immobilizing and reducing the uptake of toxic ions like Na, under stressful conditions. However, the interaction of Si and plant antioxidant enzyme system remains poorly understood, and further in-depth analyses at the transcriptomic level are needed to understand the mechanisms responsible for the Si-mediated regulation of stress responses.
••
TL;DR: An order-of-magnitude breakdown of the Wiedemann-Franz law is reported at high temperatures ranging from 240 to 340 kelvin in metallic vanadium dioxide in the vicinity of its metal-insulator transition.
Abstract: In electrically conductive solids, the Wiedemann-Franz law requires the electronic contribution to thermal conductivity to be proportional to electrical conductivity. Violations of the Wiedemann-Franz law are typically an indication of unconventional quasiparticle dynamics, such as inelastic scattering, or hydrodynamic collective motion of charge carriers, typically pronounced only at cryogenic temperatures. We report an order-of-magnitude breakdown of the Wiedemann-Franz law at high temperatures ranging from 240 to 340 kelvin in metallic vanadium dioxide in the vicinity of its metal-insulator transition. Different from previously established mechanisms, the unusually low electronic thermal conductivity is a signature of the absence of quasiparticles in a strongly correlated electron fluid where heat and charge diffuse independently.
••
TL;DR: In this paper, a ternary CdS/reduced graphene oxide (RGO)/graphitic carbon nitride (g-C3N4) hybrid photocatalyst for H2 generation and degradation of atrazine (a potent herbicide) through Z-scheme electron transport is presented.
••
Vardan Khachatryan1, Albert M. Sirunyan1, Armen Tumasyan1, Wolfgang Adam +2333 more•Institutions (195)
TL;DR: In this paper, the authors acknowledge the enduring support for the construction and operation of the LHC and the CMS detector provided by the following funding agencies:======BMWFW and FWF (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ,======And FAPESP (Brazil); MES (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS======(Colombia); MSES and CSF (Croatia); RPF (
Abstract: we acknowledge the enduring support for the construction and
operation of the LHC and the CMS detector provided by the following funding agencies:
BMWFW and FWF (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ,
and FAPESP (Brazil); MES (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS
(Colombia); MSES and CSF (Croatia); RPF (Cyprus); SENESCYT (Ecuador);
MoER, ERC IUT and ERDF (Estonia); Academy of Finland, MEC, and HIP (Finland);
CEA and CNRS/IN2P3 (France); BMBF, DFG, and HGF (Germany); GSRT (Greece);
OTKA and NIH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN
(Italy); MSIP and NRF (Republic of Korea); LAS (Lithuania); MOE and UM (Malaysia);
BUAP, CINVESTAV, CONACYT, LNS, SEP, and UASLP-FAI (Mexico); MBIE (New
Zealand); PAEC (Pakistan); MSHE and NSC (Poland); FCT (Portugal); JINR (Dubna);
MON, RosAtom, RAS and RFBR (Russia); MESTD (Serbia); SEIDI and CPAN (Spain);
Swiss Funding Agencies (Switzerland); MST (Taipei); ThEPCenter, IPST, STAR and
NSTDA (Thailand); TUBITAK and TAEK (Turkey); NASU and SFFR (Ukraine); STFC
(United Kingdom); DOE and NSF (U.S.A.).
••
TL;DR: In this paper, a metal-organic framework (MOF) was pyrolyzed at different temperatures (800, 1000, and 1200) in order to prepare porous carbons derived from MOF (PCDMs).
••
TL;DR: In this paper, the Higgs boson mass was measured in the H → ZZ → 4l (l = e, μ) decay channel and the signal strength modifiers for individual Higgs production modes were also measured.
Abstract: Properties of the Higgs boson are measured in the H → ZZ → 4l (l = e, μ) decay channel. A data sample of proton-proton collisions at $ \sqrt{s}=13 $ TeV, collected with the CMS detector at the LHC and corresponding to an integrated luminosity of 35.9 fb$^{−1}$ is used. The signal strength modifier μ, defined as the ratio of the observed Higgs boson rate in the H → ZZ → 4l decay channel to the standard model expectation, is measured to be μ = 1.05$_{− 0.17}^{+ 0.19}$ at m$_{H}$ = 125.09 GeV, the combined ATLAS and CMS measurement of the Higgs boson mass. The signal strength modifiers for the individual Higgs boson production modes are also measured. The cross section in the fiducial phase space defined by the requirements on lepton kinematics and event topology is measured to be 2. 92$_{− 0.44}^{+ 0.48}$ (stat)$_{− 0.24}^{+ 0.28}$ (syst)fb, which is compatible with the standard model prediction of 2.76 ± 0.14 fb. Differential cross sections are reported as a function of the transverse momentum of the Higgs boson, the number of associated jets, and the transverse momentum of the leading associated jet. The Higgs boson mass is measured to be m$_{H}$ = 125.26 ± 0.21 GeV and the width is constrained using the on-shell invariant mass distribution to be Γ$_{H}$ < 1.10 GeV, at 95% confidence level.
••
Vardan Khachatryan, Albert M. Sirunyan, Armen Tumasyan, Wolfgang Adam1 +2273 more•Institutions (154)
TL;DR: In this article, the second-order and third-order azimuthal anisotropy harmonics of unidentified charged particles, as well as v2v2 of View the MathML sourceKS0 and ViewTheMathML sourceΛ/Λ ǫ particles, are extracted from long-range two-particle correlations as functions of particle multiplicity and transverse momentum.
••
TL;DR: In this article, a family of titania derived nanocomposites synthesized via sol-gel and hydrothermal routes exhibit excellent performance for the photocatalytic degradation of two important exemplar water pollutants, oxytetracycline and Congo Red.
Abstract: A family of titania derived nanocomposites synthesized via sol-gel and hydrothermal routes exhibit excellent performance for the photocatalytic degradation of two important exemplar water pollutants, oxytetracycline and Congo Red. Low loadings of Co3O4 nanoparticles dispersed over the surfaces of anatase TiO2 confer visible light photoactivity for the aqueous phase decomposition of organics through the resulting heterojunction and reduced band gap. Subsequent modification of these Co3O4/TiO2 composites by trace amounts of graphene oxide nanosheets in the presence of a diamine linker further promotes both oxytetracycline and Congo Red photodegradation under simulated solar and visible irradiation, through a combination of enhanced photoresponse and consequent radical generation. Radical quenching and fluorescence experiments implicate holes and hydroxyl radicals as the respective primary and secondary active species responsible for oxidative photodegradation of pollutants.
••
TL;DR: In this paper, the performance of two-dimensional (2D) Ti3C2Tx MXene nanosheets in the adsorption and copper removal from aqueous media was investigated.
Abstract: The performance of two-dimensional (2D) Ti3C2Tx MXene nanosheets in the adsorption and copper removal from aqueous media was investigated. Delaminated (DL)-Ti3C2Tx exhibited excellent Cu removal ability, because of their large specific surface area, hydrophilicity, and unique surface functional properties. Scanning electron microscopy coupled with energy-dispersive spectroscopy (SEM–EDS), transmission electron microscopy (TEM), Brunauer–Emmett–Teller (BET), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) analyses were performed to analyze the structural changes in Ti3C2Tx MXene and its interaction with Cu ions. Oxygenated moieties in the layered structure of MXene facilitated reductive adsorption of Cu2+ forming Cu2O and CuO species. DL-Ti3C2Tx exhibited a higher and faster Cu uptake, compared to multilayer (ML)-Ti3C2Tx. The maximum experimental adsorption capacity (Qexp,max) was 78.45 mg g–1, and 80% of the total content of metal ions was adsorbed within 1 min. A pseudo-second-order k...
••
TL;DR: The first experimental demonstrations of the complete complex-amplitude holograms with subwavelength definition at visible wavelengths were achieved, and excellent performances with a remarkable signal-to-noise ratio as compared to those of traditional phase-only holograms were obtained.
Abstract: Reconstruction of light profiles with amplitude and phase information, called holography, is an attractive optical technology with various significant applications such as three-dimensional imaging and optical data storage. Subwavelength spatial control of both amplitude and phase of light is an essential requirement for an ideal hologram. However, traditional holographic devices suffer from their restricted capabilities of incomplete modulation in both amplitude and phase of visible light; this results in sacrifice of optical information and undesirable occurrences of critical noises in holographic images. Herein, we have proposed a novel metasurface that is capable of completely controlling both the amplitude and phase profiles of visible light independently with subwavelength spatial resolution. The full, continuous, and broadband control of both amplitude and phase was achieved using X-shaped meta-atoms based on the expanded concept of the Pancharatnam-Berry phase. The first experimental demonstrations of the complete complex-amplitude holograms with subwavelength definition at visible wavelengths were achieved, and excellent performances with a remarkable signal-to-noise ratio as compared to those of traditional phase-only holograms were obtained. Extraordinary control capability with versatile advantages of our metasurface paves a way to an ideal holography, which is expected to be a significant advancement in the field of optical holography and metasurfaces.
••
TL;DR: In this paper, the authors explored NK cell-derived exosome-mediated antitumor effects against aggressive melanoma in vitro and in vivo, and the results indicated that exosomes derived from NK cells exert cytotoxic effects on melanoma cells.
Abstract: Objective: Exosomes are nanovesicles that are released from normal and tumor cells and are detectable in cell culture supernatant and human biological fluids. Although previous studies have explored exosomes released from cancer cells, little is understood regarding the functions of exosomes released by normal cells. Natural killer (NK) cells display rapid immunity to metastatic or hematological malignancies, and efforts have been undertaken to clinically exploit the antitumor properties of NK cells. However, the characteristics and functions of exosomes derived from NK cells remain unknown. In this study, we explored NK cell-derived exosome-mediated antitumor effects against aggressive melanoma in vitro and in vivo. Methods: B16F10 cells were transfected with enhanced firefly luciferase (effluc) and thy1.1 genes, and thy1.1-positive cells were immunoselected using microbeads. The resulting B16F10/effluc cells were characterized using reverse transcriptase polymerase chain reaction (RT-PCR), western blotting, and luciferase activity assays. Exosomes derived from NK-92MI cells (NK-92 Exo) were isolated by ultracentrifugation and density gradient ultracentrifugation. NK-92 Exo were characterized by transmission electron microscopy and western blotting. We also performed an enzyme-linked immunosorbent assay to measure cytokines retained in NK-92 Exo cells. The in vitro cytotoxicity of NK-92 Exo against the cancer cells was determined using a bioluminescence imaging system (BLI) and CCK-8 assays. To investigate the possible side effects of NK-92 Exo on healthy cells, we also performed the BLI and CCK-8 assays using the human kidney Phoenix™-Ampho cell line. Flow cytometry and western blotting confirmed that NK-92 Exo induced apoptosis in the B16F10/effluc cells. In vivo, we used a B16F10/effluc cell xenograft model to detect the immunotherapeutic effect of NK-92 Exo. We injected NK-92 Exo into tumors, and tumor growth progression was monitored using the IVIS Lumina imaging system and ultrasound imaging. Tumor mass was monitored after in vivo experiments. Results: RT-PCR and western blotting confirmed effluc gene expression and protein levels in B16F10/effluc cells. B16F10/effluc activity was found to increase with increasing cell numbers, using BLI assay. For NK-92 Exo characterization, western blotting was performed on both ultracentrifuged and density gradient-isolated exosomes. The results confirmed that NK cell-derived exosomes express two typical exosome proteins, namely CD63 and ALIX. We demonstrated by western blot analysis that NK-92 Exo presented two functional NK proteins, namely perforin and FasL. Moreover, we confirmed the membrane expression of FasL. The enzyme-linked immunosorbent assay results indicated that NK-92 Exo can secrete tumor necrosis factor (TNF)-α, which affected the cell proliferation signaling pathway. The antitumor effect of NK-92 Exo against B16F10/effluc cells in vitro was confirmed by BLI (p < 0.001) and CCK-8 assays (p < 0.001). Furthermore, in normal healthy cells, even after 24 h of co-culture, NK-92 Exo did not exhibit significant side effects. In the in vivo experiments, tumors in the vehicle control group were significantly increased, compared with those in the NK-92 Exo-treated group (p < 0.05). Conclusion: The results of the current study suggest that exosomes derived from NK cells exert cytotoxic effects on melanoma cells and thus warrant further development as a potential immunotherapeutic strategy for cancer.
••
TL;DR: It is demonstrated that LRSL has potent antioxidative activity, decreasing ROS generation in RAW 264.7 cells and increasing the transcriptional and translational levels of antioxidant enzymes by activating Nrf2-mediated HO-1 induction via p38 signaling.
Abstract: The aim of the present study was to examine the antioxidative activity of (+)-lariciresinol (LRSL), an optically active lignan isolated from Rubia philippinensis in several in vitro assays. LRSL was also subjected to evaluate its inhibitory effect against the generation of reactive oxygen species (ROS) in murine macrophage (RAW 264.7) cells. The results showed that LRSL possessed very strong radical scavenging activity and reducing power, as well as inhibited ROS generation in a dose-dependent manner without showing any cytotoxicity. The transcriptional and translational levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were markedly higher in the sample treated group. LRSL treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with a corresponding increase in the transcriptional and translational activities of the heme oxygenase-1 (HO-1). LRSL activated p38 and treatments with SB239063 (a p38 inhibitor) suppressed the LRSL-induced activation of Nrf2, resulting in a decrease in HO-1 expression. Collectively, the data demonstrated that LRSL has potent antioxidative activity, decreasing ROS generation in RAW 264.7 cells and increasing the transcriptional and translational levels of antioxidant enzymes by activating Nrf2-mediated HO-1 induction via p38 signaling.
••
TL;DR: In this article, Nitrogen-doped carbon dots (N-CDs) were synthesized from Chionanthus retusus (C. retus) fruit extract using a simple hydrothermal carbonization method.
Abstract: Nitrogen-doped carbon dots (N-CDs) were synthesized from Chionanthus retusus (C. retusus) fruit extract using a simple hydrothermal-carbonization method. Their ability to sense metal ions, and their biological activity in terms of cell viability and bioimaging applications were evaluated. The resulting N-CDs were characterized by various physicochemical techniques such as high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Raman spectroscopy. The optical properties were characterized by ultraviolet visible (UV-vis) fluorescence spectroscopy techniques. The average size of the N-CDs was approximately 5 ± 2 nm with an interlayer distance of 0.21 nm, as calculated from the HRTEM images. The presence of phytoconstituent functionalities and the percentages of components in the N–CDs were confirmed by XPS studies, and a nitrogen content of 5.3% was detected. The N–CDs demonstrated highly durable fluorescence properties and low cytotoxicity with a quantum yield of 9%. The synthesized N–CDs were then used as probes for the detection of metal ions. The N–CDs exhibited high sensitivity and selectivity towards Fe3+, with a linear relationship between 0 and 2 μM and a detection limit of 70 μM. The synthesized N–CDs are anticipated to have diverse biomedical applications, particularly for bioimaging, given their high fluorescence, excellent water solubility, good cell permeability, and negligible cytotoxicity. Finally, the potential of N–CDs as biological probes was investigated using fungal (Candida albicans and Cryptococcus neoformans) strains via fluorescent microscopy. We found that N–CDs were suitable candidates for differential staining applications in yeast cells with good cell permeability, localization with negligible cytotoxicity. Hence, N–CDs may find dual utility as probes for the detection of cellular pools of metal ions (Fe3+) and also for early detection of opportunistic yeast infections in biological samples.
••
TL;DR: The observation of new properties of primary cosmic rays He, C, and O measured in the rigidity (momentum/charge) range 2 GV to 3 TV with 90×10−6 helium, 8.4×10^{6} carbon, and 7.0×10 6} oxygen nuclei collected by the Alpha Magnetic Spectrometer (AMS) during the first five years of operation are reported.
Abstract: We report the observation of new properties of primary cosmic rays He, C, and O measured in the rigidity (momentum/charge) range 2 GV to 3 TV with 90×106 helium, 8.4×106 carbon, and 7.0×106 oxygen nuclei collected by the Alpha Magnetic Spectrometer (AMS) during the first five years of operation. Above 60 GV, these three spectra have identical rigidity dependence. They all deviate from a single power law above 200 GV and harden in an identical way.
••
TL;DR: The composition of HA, the advances in its synthesis processes, characterization and functionalization techniques, and its importance in the biomedical field in general, and in emerging areas such as implants, drug delivery, composites, coatings, and ceramic materials in particular are highlighted.
Abstract: Hydroxyapatite (HA) is a member of the Ca apatite family. It resembles natural bone in both structure and chemical composition. HA, owing to its bioactive and biocompatible properties, has been commonly used as an implant material in bone tissue regeneration (osteogenesis), and as a drug carrier in drug and gene delivery systems. With the advances in research on the use of HA, an increasing number of researchers are exploring new synthesis processes, characterization and functionalization techniques for HA and its potential role in various fields such as magnetic resonance, controlled delivery of therapeutic drugs, cell separation, bio imaging and treatment of hyperthermia. Therefore, in this review, we highlight the composition of HA, the advances in its synthesis processes, characterization and functionalization techniques, and its importance in the biomedical field in general, and in emerging areas such as implants, drug delivery, composites, coatings, and ceramic materials in particular. The idea behind writing this review was to collect and summarize the most recent studies involving HA, so that researchers can easily find HA-related information compiled in a single document. In addition, we have also discussed the future prospects of HA. We believe that readers will not only conveniently obtain the desired information from this review, but will also get to the core of the information more easily.
••
TL;DR: The current findings suggest that the phytohormone-producing abilities of endophytic bacteria can increase plant resistance to salinity, in turn improving agricultural productivity.
••
TL;DR: It is found that it is not possible to implicate, with a high degree of confidence, rapid global CH4 emissions changes as the primary driver of recent trends when the authors' inferred OH trends and these uncertainties are considered.
Abstract: The growth in global methane (CH4) concentration, which had been ongoing since the industrial revolution, stalled around the year 2000 before resuming globally in 2007. We evaluate the role of the hydroxyl radical (OH), the major CH4 sink, in the recent CH4 growth. We also examine the influence of systematic uncertainties in OH concentrations on CH4 emissions inferred from atmospheric observations. We use observations of 1,1,1-trichloroethane (CH3CCl3), which is lost primarily through reaction with OH, to estimate OH levels as well as CH3CC3 emissions, which have uncertainty that previously limited the accuracy of OH estimates. We find a 64–70% probability that a decline in OH has contributed to the post-2007 methane rise. Our median solution suggests that CH4 emissions increased relatively steadily during the late 1990s and early 2000s, after which growth was more modest. This solution obviates the need for a sudden statistically significant change in total CH4 emissions around the year 2007 to explain the atmospheric observations and can explain some of the decline in the atmospheric 13CH4/12CH4 ratio and the recent growth in C2H6. Our approach indicates that significant OH-related uncertainties in the CH4 budget remain, and we find that it is not possible to implicate, with a high degree of confidence, rapid global CH4 emissions changes as the primary driver of recent trends when our inferred OH trends and these uncertainties are considered.