Institution
Kyushu University
Education•Fukuoka, Japan•
About: Kyushu University is a education organization based out in Fukuoka, Japan. It is known for research contribution in the topics: Population & Catalysis. The organization has 68284 authors who have published 135190 publications receiving 3055928 citations. The organization is also known as: Kyūshū Daigaku.
Topics: Population, Catalysis, Cancer, Gene, Hydrogen
Papers published on a yearly basis
Papers
More filters
••
TL;DR: In this article, the authors characterized protein-containing polyelectrolyte multilayer films of poly(styrenesulfonate) and poly(allylamine hydrochloride), fabricated by the sequential adsorption of poly-lyte and anti-immunoglobulin G (anti-IgG) on solid substrates, using atomic force microscopy (AFM), scanning electron microscopy, and Fourier transform infrared reflection−absorption spectroscopy (FTIR-RAS).
Abstract: Protein-containing polyelectrolyte multilayer films of poly(styrenesulfonate) and poly(allylamine hydrochloride), fabricated by the sequential adsorption of polyelectrolyte and anti-immunoglobulin G (anti-IgG) on solid substrates, have been characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), and Fourier transform infrared reflection−absorption spectroscopy (FTIR-RAS). Visualization of the film structure on the nanometer scale, by AFM and SEM, showed that either layered or disordered films were formed depending on the number of polyelectrolyte layers separating each protein layer. For films where each anti-IgG layer was separated by one polyelectrolyte layer, an open, disordered film structure was observed and significant protein aggregation occurred. In contrast, for films in which the anti-IgG layers were separated by five polyelectrolyte layers, a layered structure with uniform protein layers was formed. Film thicknesses determined by SEM measurements were consistent w...
310 citations
••
TL;DR: It was shown that the method proposed here would be applied to a simple one-pot coating for a uniform large area, and be useful for practical use.
Abstract: A simple and easy method to prepare super-liquid-repellent surfaces is proposed Sol-gel films were prepared by hydrolysis and condensation of alkoxysilane compounds Both surface energy and roughness were controlled using colloidal silica particles and fluoroalkylsilane When the fractional amounts of both colloidal silica and fluoroalkylsilane were optimized in the films, the film surface exhibited repellency to both water and oil Finally, it was shown that the method proposed here would be applied to a simple one-pot coating for a uniform large area, and be useful for practical use
310 citations
••
TL;DR: The perforated patch techniques will prove indispensable for electrophysiologists working on the functions and modulations of receptors, ion channels and transport phenomena and should continue to provide important information relevant to the physiological and pathological conditions of cells.
Abstract: We have reviewed the basic aspects of history, physicochemical properties, advantages, disadvantages, and applications of nystatin- and amphotericin B-perforated patch recording. Recently, we have developed a novel perforated patch technique using gramicidin [49, 132, 133]. Since gramicidin pores are permeable to Na+ and K+ but not to Cl-, it is possible to maintain the physiological concentration of intracellular Cl- and thereby to measure it in real-time from small neurons. Further improvement of the perforated patch techniques would be possible by limiting redistribution of specific intracellular ions. In light of their broad applicability, the perforated patch techniques will prove indispensable for electrophysiologists working on the functions and modulations of receptors, ion channels and transport phenomena. Research with the perforated patch should continue to provide important information relevant to the physiological and pathological conditions of cells.
309 citations
••
TL;DR: Air-stable n-type single walled carbon nanotubes with a variety of weak electron donors in the range of HOMO level between ca.
Abstract: Systematic Conversion of Single Walled Carbon Nanotubes into n-type Thermoelectric Materials by Molecular Dopants
309 citations
•
TL;DR: This study is the first to demonstrate that sensitivity to growth inhibition by gefitinib in NSCLC cell lines under basal growth condition is associated with dependence on Akt and ERK1/2 activation in response to EGFR signaling for survival and proliferation and also that drug sensitivity may be related to the extent of EGF-induced down-regulation of cell surface EGFR.
Abstract: Gefitinib (Iressa, ZD1839), a quinazoline tyrosine kinase inhibitor that targets the epidermal growth factor receptor (EGFR), is approved for patients with advanced non-small cell lung cancer (NSCLC) in several countries including Japan. However, the mechanism of drug sensitivity to gefitinib is not fully understood. In this study, we examined the molecular basis of sensitivity to gefitinib using nine human lung cancer cell lines derived from NSCLC. PC9 was the most sensitive to gefitinib of the nine NSCLC cell lines when assayed either by colony formation or MTS assays. The various cell lines expressed different levels of EGFR, HER2, HER3, and HER4, but there was no correlation between levels of EGFR and/or HER2 expression and drug sensitivity. Phosphorylation of EGFR, protein kinase B/AKT (Akt), and extracellular signal-regulated kinase (ERK) 1/2 was inhibited by much lower concentration of gefitinib in PC9 cells than in the other eight cell lines under exponential growing conditions. About 80% of cell surface EGFR in PC-9 was internalized within 10 min, whereas only about 30-50% of the cell surface EGFR was internalized in more drug-resistant cell lines in 15-60 min. The present study is the first to demonstrate that sensitivity to growth inhibition by gefitinib in NSCLC cell lines under basal growth condition is associated with dependence on Akt and ERK1/2 activation in response to EGFR signaling for survival and proliferation and also that drug sensitivity may be related to the extent of EGF-induced down-regulation of cell surface EGFR.
309 citations
Authors
Showing all 68546 results
Name | H-index | Papers | Citations |
---|---|---|---|
Tony Hunter | 175 | 593 | 124726 |
Stanley B. Prusiner | 168 | 745 | 97528 |
Yang Yang | 164 | 2704 | 144071 |
Stephen J. Elledge | 162 | 406 | 112878 |
Takashi Taniguchi | 152 | 2141 | 110658 |
Andrew White | 149 | 1494 | 113874 |
Junji Tojo | 135 | 878 | 84615 |
Claude Leroy | 135 | 1170 | 88604 |
Georges Azuelos | 134 | 1294 | 90690 |
Susumu Oda | 133 | 981 | 80832 |
Lucie Gauthier | 132 | 679 | 64794 |
Hiroshi Sakamoto | 131 | 1250 | 85363 |
Frank Caruso | 131 | 641 | 61748 |
Kiyotomo Kawagoe | 131 | 1406 | 90819 |
Kozo Kaibuchi | 129 | 493 | 60461 |