Laboratory of Molecular Biology

TL;DR: This review, contributed by scientists of complementary disciplines related to carotenoid research, covers recent advances and provides a perspective on future directions on the subjects of carotENoid metabolism, biotechnology, and nutritional and health benefits.

TL;DR: By making mutants of the Pro51 enzyme at two residues that make hydrogen bonds to the ATP substrate, it is shown that Pro51 greatly improves the strength of one of these contacts.

TL;DR: By a combination of site-directed mutagenesis of recombinant tau and in vitro phosphorylation, it is shown that AT8 requires tau protein to be phosphorylated at both serine 202 and threonine 205 (using the numbering of the longest human brain tau isoform).

TL;DR: The absence of any alterations to the protein as well as the simplicity of both the primer design and the procedure itself makes RF cloning suitable for high-throughput expression and ideal for structural genomics.

TL;DR: The purification of RAP1 by DNA affinity chromatography and the isolation of its gene from a lambda gt11 genomic library using antibodies raised against the protein suggest that R AP1 may be a transcriptional regulator that can play a role in either repression or activation of transcription, depending upon the context of its binding site.