Laboratory of Molecular Biology

About: Laboratory of Molecular Biology is a facility organization based out in Cambridge, Cambridgeshire, United Kingdom. It is known for research contribution in the topics: Gene & RNA. The organization has 19395 authors who have published 24236 publications receiving 2101480 citations.

Rat × rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG

Abstract: MONOCLONAL antibodies can be produced by cultures of permanent cell lines derived by fusion of suitable myelomas with spleen cells from immunised animals1. So far, mainly two mouse myelomas, X63-Ag8 and NSI/1-Ag4.1, have been used for this purpose1,2. When cells from immunised mice are used in the fusion, the mouse × mouse hybrid myelomas can be grown as tumours in appropriate mouse strains. We describe here the preparation and use of a new clone of a rat myeloma, which is suitable for the derivation of rat × rat hybrid myelomas producing specific rat antibodies. The spent medium of hybrid myeloma cultures usually contains 1–20 μg ml−1 antibody. The serum and ascites of the tumour-bearing mice, with few exceptions, yield 1–20 mg ml−1 of antibody, which is 1,000 times more concentrated and very convenient for larger preparations.

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

Abstract: Although electron cryo-microscopy (cryo-EM) single-particle analysis has become an important tool for structural biology of large and flexible macro-molecular assemblies, the technique has not yet reached its full potential. Besides fundamental limits imposed by radiation damage, poor detectors and beam-induced sample movement have been shown to degrade attainable resolutions. A new generation of direct electron detectors may ameliorate both effects. Apart from exhibiting improved signal-to-noise performance, these cameras are also fast enough to follow particle movements during electron irradiation. Here, we assess the potentials of this technology for cryo-EM structure determination. Using a newly developed statistical movie processing approach to compensate for beam-induced movement, we show that ribosome reconstructions with unprecedented resolutions may be calculated from almost two orders of magnitude fewer particles than used previously. Therefore, this methodology may expand the scope of high-resolution cryo-EM to a broad range of biological specimens. DOI: http://dx.doi.org/10.7554/eLife.00461.001

Term amniotic membrane is a high throughput source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro

Abstract: Background Term Amniotic membrane (AM) is a very attractive source of Mesenchymal Stem Cells (MSCs) due to the fact that this fetal tissue is usually discarded without ethical conflicts, leading to high efficiency in MSC recovery with no intrusive procedures. Here we confirmed that term AM, as previously reported in the literature, is an abundant source of hMSCs; in particular we further investigated the AM differentiation potential by assessing whether these cells may also be committed to the angiogenic fate. In agreement with the recommendation of the International Society for Cellular Therapy, the mesenchymal cells herein investigated were named Amniotic Membrane-human Mesenchymal Stromal Cells (AM-hMSC).