Ladoke Akintola University of Technology

About: Ladoke Akintola University of Technology is a education organization based out in Ogbomoso, Nigeria. It is known for research contribution in the topics: Population & Adsorption. The organization has 2786 authors who have published 3066 publications receiving 36850 citations. The organization is also known as: Oyo State University of Technology & LAUTECH.

Reliable and rapid characterization of functional FCN2 gene variants reveals diverse geographical patterns

Abstract: Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility. We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165). We observed that the genotype distribution of three functional SNP variants (−986 G > A, -602 G > A and -4A > G) differ significantly between the populations investigated (p < 0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001). The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping.

Prevalence and association of human parvovirus B19V with hepatitis B and C viruses in Nigeria.

Abstract: Co-infection of parvovirus B19 with hepatitis B virus has been found in patients with acute and chronic hepatitis. The clinical significance of parvovirus B19 in hepatitis B co-infected patients is still controversial. In this study parvovirus B19 antibodies and DNA were investigated in serum samples from 76 patients with HBV infection, 17 with HBV/HCV co-infection and 44 healthy controls. In the sera from patients with HBV infection, anti-B19V IgM and IgG antibodies were detected in 24/76 (32%) and 25/76 (33%), in 6/17 (35%) and 8/17 (47%) of HBV/HCV co-infected patients, and in 14/44 (32%) and 12/44 (12%) of a non-hepatitis healthy controls, respectively. B19V DNA was detected in 8/76 (11%) of patients with HBV infection and in 3/17 (18%) of patients with a HBV/HCV co-infection, and in 4/44 (9%) healthy controls. The occurrence of parvovirus B19 DNA was significantly higher in patients with symptomatic HBV 4/20 (20%) compared to asymptomatic HBV carrier 4/56 (7%) (P<0.05). Ten of the positive B19V DNA sequences belonged to B19V genotype 1 while two belonged to genotype 3. The results of this study showed a significant difference in the prevalence of parvovirus B19 DNA in symptomatic HBsAg positive as compared to asymptomatic HBsAg positive individuals; however, the conclusion that parvovirus B19 infection increased the frequency of liver disease was not supported. Long-term longitudinal studies are, however, required to determine the synergistic effect of parvovirus B19 infection in HBV or HBV and HCV co-infected persons.

Susceptibility of the selected crops in storage to sitophilus zeamais motschulsky in southwestern nigeria

Abstract: Susceptibility of the selected Nigerian cultivars of twelve crops to Sitophilus zeamais Motschulsky was evaluated in the laboratory (28 ± 2°C temperature and 69 ± 5% relative humidity). The crops were: maize (Zea mays L.), millet (Pennisetum glaucum (L.) X R. Br.), sorghum (Sorghum bicol- or (L.) Moench), rice (Oryza sativa L.), and yam (Dioscorea rotundata Poir). Others were cassava (Mani- hot esculenta Crantz), pepper (Capsicum annuum L.), cowpea (Vigna unguiculata (L.) Walp), groundnut (Arachis hypogaea L.), melon (Citrullus lanatus (Thunb.)), soybean (Glycine max (L.) Merr.) and bambara groundnut (Vigna subterranea (L.) Verdc). The result of antixenosis prescreen shows that S. zeamais preferred cereals and tubers to legumes and oil crops. S. zeamais preference for maize was highest at 1, 24 and 48 hours after infestation (HAI) and was not significantly different from its preference for pepper, millet, sorghum and yam. At 48 HAI, S. zeamais preference for cereals, tubers and pepper was not significantly different. Soybean and bambara groundnut were the least preferred species. The highest level of damage was observed in cereals and tubers. Millet suffered significantly greater damage than maize at 2-8 weeks after infestation (WAI). Damage done to maize was not signifi- cantly different from damage done to tubers at 6 and 8 WAI. Pepper, legumes and oil crops suffered significantly lower levels of damage than maize throughout the experimental period. Cumulative number of adult was significantly higher in small-seeded cereals than maize and was of the order: sorghum>millet>rice. Cowpea, soybean and pepper did not support reproduction and longevity of S. zeamais. Longevity was best supported by cassava. The results show that in storage, cereals and tubers were more susceptible to S. zeamais infestation than legumes, spices and oil crops.

Optimization of oil extraction from Pitanga (Eugenia uniflora L.) leaves using simplex centroid design

Abstract: Simplex centroid design (SCD) was employed to optimize the mixing of petroleum ether, n-hexane, methanol and ethanol for the extraction of oil (PLO) from Pitanga (Eugenia uniflora L.) leaves, via Soxhlet extraction. The highest yield (54%) of Pitanga leaf oil (PLO) was obtained with 100% ethanol and the lowest yield (16%) from the mixture of methanol (33.3%)/hexane (33.3%)/ethanol (33.3%). The coefficient of determination (R2) of the model equation obtained was 0.91, while the adjusted R2 and predicted R2 were 0.8729 and 0.951, respectively. The saponification value (S.V.), iodine value, acid value and free fatty acid (FFA) are 35.34 mgKOH/g, 72.97 mgI2/g oil, 32.41 and 16.30 mgKOH/g, respectively.

Perceptions of Nigerian Women about Human Papilloma Virus, Cervical Cancer, and HPV Vaccine

Abstract: Background. Cervical cancer caused by human papilloma virus (HPV) though preventable has claimed the lives of many women worldwide. This study was embarked upon to evaluate the general knowledge and perceptions of Nigerian women on HPV, cervical cancer, and HPV vaccine. Methods. Structured questionnaires were administered to a cross section of 737 women randomly selected from the general population in two southwestern States of Nigeria. Statistical analysis was done using SPSS computer software version 16. A P value >0.05 was considered statistically significant. Results. One hundred and seventy-six (23.9%) of the respondents had knowledge of HPV; 474 (64.3%) are aware of cervical cancer but only 136 (18.5%) know that HPV causes cervical cancer. 200 (27.1%) are aware that there is an HPV vaccine while 300 (40.7%) had knowledge of Pap smear test. Two hundred and sixty (35.3%) of the respondents know that early detection of HPV can prevent cervical cancer and in spite of this, only 110 (14.9%) have taken the Pap smear test before while 151 (20.5%) are not willing to go for the test at all. Conclusions. There is therefore the need to create proper awareness on the HPV and its possible consequence of cervical carcinoma.