Manipal University

About: Manipal University is a education organization based out in Manipal, Karnataka, India. It is known for research contribution in the topics: Population & Medicine. The organization has 9525 authors who have published 11207 publications receiving 110687 citations.

Asymmetry analysis of breast thermograms using automated segmentation and texture features

Abstract: In this article, we present a new approach for breast thermogram image analysis by developing a fully automatic segmentation of right and left breast for asymmetry analysis, using shape features of the breast and Polynomial curve fitting. Segmentation results are validated with their respective Ground Truths. Histogram and grey level co-occurrence matrix-based texture features are extracted from the segmented images. Statistical test shows that features are highly significant in detection of breast cancer. We have obtained an accuracy of 90%, sensitivity of 87.5% and specificity of 92.5% for a set of eighty images with forty normal and forty abnormal using SVM RBF classifier.

Radiomodifying and anticlastogenic effect of Zingerone on Swiss albino mice exposed to whole body gamma radiation.

Abstract: The radioprotective effect and antigenotoxic potential of phenolic alkanone, Zingerone (ZO) were investigated in Swiss albino mice exposed to gamma radiation. To study the optimum dose for radiation protection, mice were administered with ZO (10–100 mg/kg b.wt.), once daily for five consecutive days. One hour after the last administration of ZO on the fifth day, animals were whole body exposed to 10 Gy gamma radiations. The radioprotective potential was assessed using animal survival at an optimal ZO dose of 20 mg/kg b.wt., administered prior to 7–11Gy. Further, the radioprotective potential of ZO was also analyzed by haemopoietic stem cell survival (CFU) assay, mouse bone marrow micronucleus test and histological observations of intestinal and bone marrowdamage. Effect of ZO pretreatment on radiation-induced changes in glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation (LPx) levels was also analyzed. ZO treatment resulted increase in the LD50/30 by 1.8Gy (dose reduction factor = 1.2). The number of spleen colonies after whole body irradiation of mice (4.5 or 7.5 Gy) was increased when ZO was administered 1 h prior to irradiation. The histological observations indicated a decline in the villus height and crypt number with an increase in goblet and dead cell population in the irradiated group, which was normalized by pretreatment with ZO. A significant (p < 0.001) reduction in micronucleated polychromatic, normochromatic erythrocytes, increased PCE/NCE ratio, increase in the GSH, GST, SOD, CAT and decreased LPx levelswere observed in ZO pretreated group when compared to the irradiated animals. Our findings demonstrate the potential of ZO in mitigating radiation-induced mortality and cytogenetic damage, whichmay be attributed to inhibition radiation-induced decline in the endogenous antioxidant levels and scavenging of radiation-induced free radicals.