scispace - formally typeset
Search or ask a question
Institution

Marche Polytechnic University

EducationAncona, Italy
About: Marche Polytechnic University is a education organization based out in Ancona, Italy. It is known for research contribution in the topics: Population & Prostate cancer. The organization has 5905 authors who have published 15769 publications receiving 382286 citations. The organization is also known as: Universitá Politecnica delle Marche & Universita Politecnica delle Marche.


Papers
More filters
Journal ArticleDOI
TL;DR: The germline NF1 mutational spectra comprise intragenic mutations and deletions in individuals with pheochromocytoma and NF1, and tended to cluster in the CSR over the RAS-GAP domain, suggesting that CSR plays a more prominent role in Individuals with NF1-pheochromcytoma than in NF1 individuals without this tumor.
Abstract: BACKGROUND: Neurofibromatosis type 1 (NF1) is a pheochromocytoma-associated syndrome. Because of the low prevalence of pheochromocytoma in NF1, we ascertained subjects by pheochromocytoma that also had NF1 in the hope of describing the germline NF1 mutational spectra of NF1-related pheochromocytoma. MATERIALS AND METHODS: An international registry for NF1-pheochromocytomas was established. Mutation scanning was performed using denaturing HPLC for intragenic variation and quantitative PCR for large deletions. Loss-of-heterozygosity analysis using markers in and around NF1 was performed. RESULTS: There were 37 eligible subjects (ages 14-70 yr). Of 21 patients with corresponding tumor available, 67% showed somatic loss of the nonmutated allele at the NF1 locus vs. 0 of 12 sporadic tumors (P = 0.0002). Overall, 86% of the 37 patients had exonic or splice site mutations, 14% large deletions or duplications; 79% of the mutations are novel. The cysteine-serine rich domain (CSR) was affected in 35% but the RAS GTPase activating protein domain (RGD) in only 13%. There did not appear to be an association between any clinical features, particularly pheochromocytoma presentation and severity, and NF1 mutation genotype. CONCLUSIONS: The germline NF1 mutational spectra comprise intragenic mutations and deletions in individuals with pheochromocytoma and NF1. NF1 mutations tended to cluster in the CSR over the RAS-GAP domain, suggesting that CSR plays a more prominent role in individuals with NF1-pheochromocytoma than in NF1 individuals without this tumor. Loss-of-heterozygosity of NF1 markers in NF1-related pheochromocytoma was significantly more frequent than in sporadic pheochromocytoma, providing further molecular evidence that pheochromocytoma is a true component of NF1.

122 citations

Journal ArticleDOI
TL;DR: It is proposed that the mitochondrial carbonic anhydrase is involved in supplying HCO3- for anaplerotic assimilation catalyzed by phosphoenolpyruvate carboxylase, which provides C skeletons for N assimilation under some circumstances.
Abstract: Previous studies of the mitochondrial carbonic anhydrase (mtCA) of Chlamydomonas reinhardtii showed that expression of the two genes encoding this enzyme activity required photosynthetically active radiation and a low CO 2 concentration. These studies suggested that the mtCA was involved in the inorganic carbon-concentrating mechanism. We have now shown that the expression of the mtCA at low CO 2 concentrations decreases when the external NH 4 + concentration decreases, to the point of being undetectable when NH 4 + supply restricts the rate of photoautotrophic growth. The expression of mtCA can also be induced at supra-atmospheric partial pressure of CO 2 by increasing the NH 4 + concentration in the growth medium. Conditions that favor mtCA expression usually also stimulate anaplerosis. We therefore propose that the mtCA is involved in supplying HCO 3 - for anaplerotic assimilation catalyzed by phospho enol pyruvate carboxylase, which provides C skeletons for N assimilation under some circumstances.

121 citations

Journal ArticleDOI
TL;DR: The available information strongly suggests that S. suis is an important antibiotic resistance reservoir that can contribute to the spread of resistance genes to the above-mentioned streptococci, and is thus a paradigmatic example of possible intersections between animal and human resistomes.
Abstract: Streptococcus suis, a major porcine pathogen, has been receiving growing attention not only for its role in severe and increasingly reported infections in humans, but also for its involvement in drug resistance. Recent studies and the analysis of sequenced genomes have been providing important insights into the S. suis resistome, and have resulted in the identification of resistance determinants for tetracyclines, macrolides, aminoglycosides, chloramphenicol, antifolate drugs, streptothricin, and cadmium salts. Resistance gene-carrying genetic elements described so far include integrative and conjugative elements, transposons, genomic islands, phages, and chimeric elements. Some of these elements are similar to those reported in major streptococcal pathogens such as Streptococcus pyogenes, Streptococcus pneumoniae, and Streptococcus agalactiae and share the same chromosomal insertion sites. The available information strongly suggests that S. suis is an important antibiotic resistance reservoir that can contribute to the spread of resistance genes to the above-mentioned streptococci. S. suis is thus a paradigmatic example of possible intersections between animal and human resistomes.

121 citations

Journal ArticleDOI
TL;DR: In this paper, the authors investigated whether antioxidant activity of tea may be affected by hot or cold water steeping and if this correlates with their polyphenol content and metal-chelating activity.

121 citations

Journal ArticleDOI
TL;DR: An intriguing and innovative hypothesis is that the dysfunction of TLRs signaling and the acquisition of SASP can be two interconnected phenomena that appear accelerated in presence of the majors age-related diseases, such as cardiovascular diseases, diabetes, neurodegenerative diseases and cancers.
Abstract: The age-related changes of immune system functions are complex phenomena incompletely understood The acquired immune system shows a functional decline in ability to respond to new pathogens during aging, whereas serum levels of inflammatory cytokines are increased with age The source of this age-related systemic chronic inflammation, named inflammaging, was mainly attributed to the progressive activation of immune cells over time However, recent studies have shown that the process of cellular senescence can be an important additional contributor to chronic inflammation, since senescent cells acquire a phenotype named “senescence-associated secretory phenotype” (SASP), characterized by the enhanced secretion of many inflammation modulators Pathogen-associated molecular pattern receptors, in particular Toll-like receptors (TLRs), are key molecules in the response of innate immunity cells to pathological stimuli An intriguing and innovative hypothesis is that the dysfunction of TLRs signaling and the acquisition of SASP can be two interconnected phenomena The TLR family, including receptors and co-effector molecules, do not show a consistent age-dependent change across model systems However, there is evidence for impaired downstream signaling events, including inhibition of positive and activation of negative modulators of TLR signaling MicroRNAs (miRNAs) are a newly discovered class of gene regulators acting as post-transcriptional repressors of a number of genes The miRNA property to finely-tune gene expression makes them right for immune system regulation, which requires precise control for proper activity We reviewed evidences suggesting that miRNAs can modulate TLR signaling mainly by three different mechanisms: 1) miRNAs can directly target components of the TLR signaling system, 2) miRNA expression can be directly regulated by TLRs pathway activation and 3) miRNAs can directly activate the RNA-sensing TLRs, like TLR-8, in humans We also reviewed how TLR signaling is modulated by miRNAs during aging, and how an impaired miRNAs/TLR signaling interaction in immune system cells and related cells, ie endothelial cells and adipocytes, can contribute to inflammaging observed in normal aging Interestingly, this impairment appears accelerated in presence of the majors age-related diseases, such as cardiovascular diseases, diabetes, neurodegenerative diseases and cancers

121 citations


Authors

Showing all 6013 results

NameH-indexPapersCitations
Jonathan I. Epstein138112180975
Antoni Ribas13266099227
Francesco Fiori128103276699
Claudio Franceschi12085659868
Robert E. Coleman10372449796
Carmine Zoccali9981336774
Massimo Falconi9466741966
Mario Plebani91132943055
Roberto Danovaro8441523735
Rodolfo Montironi8395830957
Diego Centonze8146322857
Saverio Cinti7825632760
Michele Brignole7639926758
Jürgen P. Rabe7639120174
Jean-Jacques Body7038419608
Network Information
Related Institutions (5)
University of Florence
79.5K papers, 2.3M citations

95% related

University of Bologna
115.1K papers, 3.4M citations

95% related

University of Padua
114.8K papers, 3.6M citations

94% related

Sapienza University of Rome
155.4K papers, 4.3M citations

94% related

University of Pisa
73.1K papers, 2.1M citations

94% related

Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
202376
2022181
20211,353
20201,390
20191,289
20181,148