Marche Polytechnic University

About: Marche Polytechnic University is a education organization based out in Ancona, Italy. It is known for research contribution in the topics: Population & Cancer. The organization has 5905 authors who have published 15769 publications receiving 382286 citations. The organization is also known as: Universitá Politecnica delle Marche & Universita Politecnica delle Marche.

MiR-146a as marker of senescence-associated pro-inflammatory status in cells involved in vascular remodelling

Abstract: In order to identify new markers of vascular cell senescence with potential in vivo implications, primary cultured endothelial cells, including human umbilical vein endothelial cells (HUVECs), human aortic endothelial cells (HAECs), human coronary artery endothelial cells (HCAECs) and ex vivo circulating angiogenic cells (CACs), were analysed for microRNA (miR) expression. Among the 367 profiled miRs in HUVECs, miR-146a, miR-9, miR-204 and miR-367 showed the highest up-regulation in senescent cells. Their predicted target genes belong to nine common pathways, including Toll-like receptor signalling (TLR) that plays a pivotal role in inflammatory response, a key feature of senescence (inflammaging). MiR-146a was the most up-regulated miR in the validation analysis (>10-fold). Mimic and antagomir transfection confirmed TLR’s IL-1 receptor-associated kinase (IRAK1) protein modulation in both young and senescent cells. Significant correlations were observed among miR-146a expression and β-galactosidase expression, telomere length and telomerase activity. MiR-146a hyper-expression was also validated in senescent HAECs (>4-fold) and HCAECs (>30-fold). We recently showed that CACs from patients with chronic heart failure (CHF) presented a distinguishing feature of senescence. Therefore, we also included miR-146a expression determination in CACs from 37 CHF patients and 35 healthy control subjects (CTR) for this study. Interestingly, a 1,000-fold increased expression of miR-146a was observed in CACs of CHF patients compared to CTR, along with decreased expression of IRAK1 protein. Moreover, significant correlations among miR-146a expression, telomere length and telomerase activity were observed. Overall, our findings indicate that miR-146a is a marker of a senescence-associated pro-inflammatory status in vascular remodelling cells.

Mechanical properties of FRCM using carbon fabrics with different coating treatments

Abstract: The use of composite materials for repair and retrofit of structures has become a common use among the engineering community. Fabric Reinforced Cementitious Matrix (FRCM) is a composite material specifically designed for masonry and concrete rehabilitation and is becoming a viable alternative to FRP, whenever the project conditions do not allow the use of organic polymer based composites. FRCM is usually constituted by one or multiple plies of dry fabrics (carbon, glass, aramid, basalt or PBO fibers) embedded into an inorganic matrix. If a polymer is used to either cover or bond the fabric strands, such polymer does not fully penetrate and impregnate the fibers as it would for FRP. The purpose of this research work is to study how different types and amounts of organic coatings applied to a carbon fabric could affect the bond behavior between fabric and mortar. The effectiveness of coating treatments was studied by means of direct tensile, pull-off and shear-bond double-lap tests. Experimentation was carried out on different combinations of fabrics and mortars, by varying the levels of pre-impregnation of the fabric during its manufacturing. In addition, the use of a quartz sand layer applied to the fabric after impregnation was investigated. Experimental evidence shows a promising enhancement of the bond between fabric and matrix and, therefore, of the entire system even with the use of low percentages of resin, depending on the type of mortar used.

snRNA-seq reveals a subpopulation of adipocytes that regulates thermogenesis.

Abstract: Adipose tissue is usually classified on the basis of its function as white, brown or beige (brite)1. It is an important regulator of systemic metabolism, as shown by the fact that dysfunctional adipose tissue in obesity leads to a variety of secondary metabolic complications2,3. In addition, adipose tissue functions as a signalling hub that regulates systemic metabolism through paracrine and endocrine signals4. Here we use single-nucleus RNA-sequencing (snRNA-seq) analysis in mice and humans to characterize adipocyte heterogeneity. We identify a rare subpopulation of adipocytes in mice that increases in abundance at higher temperatures, and we show that this subpopulation regulates the activity of neighbouring adipocytes through acetate-mediated modulation of their thermogenic capacity. Human adipose tissue contains higher numbers of cells of this subpopulation, which could explain the lower thermogenic activity of human compared to mouse adipose tissue and suggests that targeting this pathway could be used to restore thermogenic activity.