Military Academy

About: Military Academy is a based out in . It is known for research contribution in the topics: Population & Context (language use). The organization has 2478 authors who have published 3003 publications receiving 33188 citations.

Detection and species identification of Old World Leishmania in clinical samples using a PCR-based method

Abstract: The aim of this study was to develop a simple, low-cost method for the detection and species differentiation of Leishmania directly from clinical samples, for routine use in a parasitology laboratory. A total of 87 samples was used, including 60 peripheral blood, seven bone marrow and 17 skin lesion material samples, derived from Greek patients with visceral or cutaneous leishmaniasis, and three reference strains. PCR was performed using primers designed to amplify the internal transcribed spacer 1 (ITS1) region of the rRNA gene. Identification of the Leishmania species studied was achieved by digestion with a single restriction endonuclease (RFLP), single-strand conformational polymorphism (SSCP) and DNA sequencing of the PCR-generated fragments. Typing identified all visceral and one cutaneous leishmaniasis strains as L. infantum, twelve of the cutaneous leishmaniasis strains as L. tropica and four as L. major. The described PCR method proved efficient for the detection of pathogenic Leishmania species in various clinical samples, most importantly in peripheral blood samples. Furthermore, PCR followed by a simple RFLP using a single restriction endonuclease was capable of identifying all Leishmania species commonly encountered in Greece.

Design and fabrication of flexible piezo-microgenerator by depositing ZnO thin films on PET substrates

Abstract: In this study, a new design of PET-based (polyethylene terephthalate) flexible power harvesting system is proposed. It consists of flexible PET vibration substrate, piezoelectric ZnO (zinc oxide) thin film, design of lump structures and electrodes. The relationship between the dynamic response of PET substrate and its power output is realized. High electromechanical transformation of piezoelectric material and efficient energy transfer of mechanical structure make the piezoelectric generator a high performance. The flexible devices of cantilevers with lump structures and piezoelectric ZnO thin film is designed. A layer of aluminum (Al) thin film is placed as a top electrode and a copper (Cu) foil as a bottom electrode. The piezoelectric cantilever plate is designed by using ANSYS® software. Both modal analysis and harmonic response analysis are performed to obtain the structural modal parameters and frequency response functions, respectively. Then, imprinting process is applied to transfer the configuration of geometric lump structure, based on the simulated result, onto the flexible substrate to improve the power output. The lump material is ultraviolet-curable (UV) resin. The high-quality ZnO piezoelectric thin film has an even distribution of crystal grains. The angle obtained from diffraction diagram approaches 34.5°. It was deposited to the Al/PET flexible substrate by using RF (radio frequency) magnetron sputtering under 75 W power. In comparison of simulation and experiments with and without the lump structure, the lump structures on the flexible generator do improve the power output. The generator achieves maximum OCV (open-circuit voltage) up to 2.25 V and CCV (closed-circuit voltage) up to 1.55 V which is 0.276 μW per centimeter square. In addition, the devices are fabricated by a new process where the piezoelectric active areas on the cantilever are supported to achieve a large displacement with low residual stress. With low elastic constant of the PET-based layer, the cantilever structure can achieve better displacement compared with the conventional SOI (silicon on insulator) structures or other metal substrates.