Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Chemistry & Salmonella. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Nachweis von organischen Oxydantien in der Tüpfelanalyse

Abstract: Gewisse organische Oxydantien konnen durch Tupfelreaktionen auf mit Thio-Michlers Keton oder Tetrotan impragniertem Fallerpapier nachgewiesen werden. Es entstehen blaue Flecke. Die Erfassungsgrenzen der Nachweise liegen zwischen 0,04 und 1,2 μg. N-Brom-(Chlor)-succinimid reagiert mit alkalischen TlI-Salzlosungen unter Bildung von braunem Tl O(OH), das einen selektiven Nachweis dieser Verbindungen ermoglicht. Chloramin T kann in alkalischer Losung neben Alkalihypochlorit nachgewiesen werden nach Zersetzung des Hypochlorites mit Wasserstoffperoxyd und Zinkchlorid und darananschliesender Farbreaktion mit Thio-Michlers Keton.

Closure system for a children's drinking bottle or a children's drinking cup

Abstract: Closure system for a children's drinking bottle or a children's drinking cup comprising a sealing ring which has on an external periphery a fastening means for sealingly and releasably connecting to a container edge of a container of the children's drinking bottle or the children's drinking cup, an aperture for receiving a mouthpiece and/or a blind lid and an internal thread, which borders the aperture and is arranged at a distance from the aperture and the fastening means and a fixing insert with a through aperture and an external thread which may be screwed into the internal thread of the sealing ring in order to clamp sealingly a fastening attachment of the mouthpiece and/or the blind lid between the fixing insert and the sealing ring.

Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata

Abstract: The bovine taeniasis-cysticercosis complex can be defined as a set of pathological changes caused by the adult form of Taenia saginata in humans and their larval form in animals ( Cysticercus bovis ). Data on the occurrence of bovine cysticercosis comes from the records of veterinary inspection of meat in slaughterhouses under fiscalization, where some positive cases may go unnoticed, especially in moderate infections. So, it is relevant the use of serological tests that have greater sensitivity than the post-mortem routine exams. Studies have shown the possible application of the ELISA test as a tool for epidemiological studies of the parasitosis and in the identification of animals with cysts. The aim of this study is to determine the detection threshold of the indirect ELISA test, using experimentally and naturally infected animals in detecting cases of cysticercosis. The sensitivity of the test to naturally infected animals using a cut-off 1 and 2 added with 2 standart-deviation (SD) was of 12% and 24.4%, respectively. However, when using the cut-off of 1 and 2 added with 3 SD, the test sensitivity dropped, represented by 14.4% and 1.99% of the test sensitivity. As for the samples from experimentally infected animals, using the cut-off 1 and 2 added with 2 SD the sensitivity was 55.9% and 92.5%; and adding 3 SD the values found were 31.2% and 86%, respectively. The specificity of the test in all situations tested was 100%. It is important to take into account the right choice of control sera used in the ELISA test, whereas, according to its application, it is necessary to increase the sensitivity or specificity.

Simulação do dano causado por larvas de Oryzophagus oryzae (Coleoptera: Curculionidae) a cultivares de arroz irrigado

Abstract: Oryzophagus oryzae (Costa Lima, 1936) (Coleoptera: Curculionidae) is the most damaging insect in flooded rice in the South Region of Brazil. Its damage to rice plants is caused primarily by larval pruning of the root system. Two field experiments were conducted to simulate the larval damage, testing an equipment whose main part is a U form metal blade, with 6cm of width and 8cm of length, to cut the roots. The roots of cultivars, with biological early cycle and susceptibles to O. oryzae (BR-Irga 414 and Bluebelle) and with medium cycle and resistants (BR-Irga 410 and Dawn), were submitted to (1) artificial cutting by the simulator equipment, (2) natural cutting by larvae and (3) without artificial or natural cutting, protected by insecticide. The simulation was done during the rice plants vegetative phase at 31 days after permanent flood, when the larval population peak occurs. For evaluation were determined data related to weight, volume and length of roots (soon after simulation and plants booting phase), larval population on natural damaged plants and grain yield. It was observed that soon after simulation, in all cultivars, the root damage rates induced by the simulator equipment were similar to those caused by natural larval cutting. At the booting phase, also the root system recovery rates of the plants submitted to natural or artificial damage were similar. The medium cycle cultivars showed significantly higher root recovery and lower yield reduction, after both root damage types, corroborating with their higher resistance degree to O. oryzae. It was concluded that the methodology used to simulate O. oryzae larval root damage is applicable for researching genetic resistance to the insect, mainly to identify irrigated rice genotypes.