Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Chemistry & Salmonella. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Estudo anatomopatológico em tecidos condenados pelo serviço de inspeção federal (sif) por suspeita de tuberculose

Abstract: The methodology used during the Animal Standard Meat Inspection (SIS) has been controversial in the decision to condemn or approve materials for human consumption. The aim of this study was to identify the microscopic lesions found in lymph node tissues condemned for tuberculosis by the Brazilian SIS. Sixty-one condemned lymph nodes were collected, fixed in buffered formalin 10% and submitted to histological processing. By evaluating the condemned tissues with HE meat inspection; Mycobacterium bovis ; tuberculosis.

Prevalência de anticorpos antileptospira em machos bovinos na microrregião de goiânia

Abstract: The aim of this experiment was to study the prevalence of sera agglutinins anti-leptospira in bulls in Goiânia Micro-Region. Sixty farms were chosen randomly in according to sorovars presented and the tax of prevalence of them. The correlation of the prevalence of the leptospirosis were establish with the type of herd (beef or dairy) and the occurrence of abortions in farms. One hundred forty samples bulls from “Goiânia Micro-Region” were analyzed (MG) regarding 60 properties. The sera reagents prevalence was 74.28% and . presented titles with variation of dilution from 1:100 to 1:800. The prevalence of the sera varieties, presents in this “Goiania Micro-Region”, was the following: wolffi (19.23%); hardjo (15.38%); djasiman and grippotyphosa (5.76%); shermani (4.80%); patoc (1.92%); andamana, castellonis, copenhageni, hebdomadis, sentot and tarassovi (0.96%). According to these results, it was possible to conclude that leptospirosis is an endemic agent in this part of Goias State and there were not any relationship with production system or abortions and prevalence. KEY WORDS: Leptospirosis, cattle, bull, prevalence.

Experimental infection by Salmonella enterica subsp enterica serovar kottbus in day-old broiler chickens

Abstract: The strain used in this work was a Salmonella enterica subsp enterica serovar Kottbus (6,8:e,h:1,5) isolated from imported day-old ducklings in Laboratorio Nacional Agropecuario (LANAGRO/SP) of the Ministry of Agriculture of Brazil (MAPA). In view of the lack of information available about this Salmonella isolate and also because it was detected in day-old imported birds, this study was carried out to investigate the dissemination of S. Kottbus among newly hatched chicks. The birds were placed in three groups: one group of 20 birds received 0.1 mL of S. Kottbus culture containing 1.2 x 108 CFU/mL, the second group of 20 birds was inoculated with 1.2 x 105 CFU/mL and the third group of 10 birds was untreated (control group). Results were similar for both infected groups. The bacterium was recovered from cloacal swabs collected from the first day following the experimental infection until the end of the trial (42 days post-inoculation). At 15 and 42 days post-inoculation (dpi), half of the birds of each group were killed for bacteriological examination of cecal contents, liver and spleen. At 15 dpi, viable cell counts of S. Kottbus were obtained in all kinds of samples. At 42 dpi, Salmonella was present in the liver and spleen of few birds, but in large amounts in the cecal contents of almost all birds.

Simultaneous Determination of Fenproporex, Diethylpropione and Methylphenidate in Oral Fluid by LC-MS/MS

Abstract: A liquid chromatography–tandem mass spectrometry method has been developed and validated for confirmation and quantification of three amphetamine-type stimulants (ATS) in oral fluid (OF), namely fenproporex (FEN), diethylpropione (DIE) and methylphenidate (MPH), which are misused by some Brazilian drivers and have potentially dangerous consequences in road traffic. In this method, the OF was extracted by a simple and fast liquid–liquid extraction using acetonitrile. Calibration curves were linear throughout the concentration range from 2.5 to 90 ng mL−1 in OF, using internal standard. The lower limit of quantification and the limit of detection were 2.5 and 0.5 ng mL−1, respectively, for all ATS. Intra- and inter-assay precision and accuracy values were within regulatory limits. The analyte extraction presented poor recoveries, but was considered appropriated for analyses of ATS in OF due to the low limits of detection. Matrix effects and carry over were not detected. All analytes were stable during the whole analytical procedure. The present analytical method was considered simple and sensitive for simultaneous determination of FEN, DIE, and MPH in OF and suitable for clinical and forensic toxicology.