Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Biology & Chemistry. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Concentrados protéicos para bovinos: 1. Digestibilidade in situ da matéria seca e da proteína bruta

Abstract: O objetivo deste trabalho foi determinar a degradacao ruminal, pela tecnica in situ, da materia seca (MS) e da proteina bruta (PB) de 10 concentrados proteicos. As degradacoes potenciais da MS e da PB das farinhas de origem vegetal, soja, algodao, mamona e palmiste, mostraram-se elevadas (proximas a 100%), porem em funcao de taxas de degradacao mais altas para o farelo de soja (10%) e menores para o algodao (4%), mamona (MS:3%; PB:1,2%) e palmiste (1,7%), as degradabilidades efetivas (DE) foram bem superiores para o farelo de soja, independentemente da taxa de passagem, o que a torna a fonte proteica de maior disponibilidade ruminal. O gluten de milho mostrou ser uma fonte proteica de baixa degradabilidade ruminal (DE da PB: 16% para 0,05 de taxa de passagem). Dentre os alimentos de origem animal, a maior degradabilidade potencial da proteina bruta foi verificada para a farinha de carne e ossos ( 75,5%), seguida das farinhas de peixe I (58,5%), de penas e visceras (52,3%) e de sangue (36,7%). A maior degradabilidade efetiva para a taxa de passagem de 5% foi a da farinha de carne e ossos (51%), seguida da farinha de peixe I (41%), de penas e visceras (40,0%) e de sangue (33%). A farinha de peixe II apresentou valores muito baixos de degradabilidade, apenas 22% com 48h de incubacao ruminal.

Development of a differential multiplex PCR assay for equine herpesvirus 1 and 4 as a diagnostic tool.

Abstract: Summary In this study, a multiplex polymerase chain reaction (PCR) procedure was developed for differentiation of strains and field isolates of equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4). Specific oligonucleotide primers were combined to amplify the thymidine kinase (TK) gene region of EHV-1 and EHV-4, which would yield fragments of different lengths for each virus in the same amplification reaction. The specificity of the largest PCR amplicon for EHV-4 was confirmed by restriction digestion with HindIII. The multiplex PCR proved to be a fast and sensitive method for typing EHV-1 and EHV-4 isolates and for detection and differentiation of both viruses in field samples in which infectious virus is no longer available. The sensitivity was improved by combining cycling optimization and visualization of PCR products in ethidium bromide and silver-stained acrylamide gels.

Adubação nitrogenada de cobertura no feijoeiro, em plantio direto e convencional

Abstract: The objective of this work was to evaluate the effect of nitrogen topdressing in common bean agronomic performance in no tillage and conventional tillage system. The work was developed during two seasons, in different years, in a Rhodic Kandiudox soil, using the succession black oat/pear millet/common bean cv. Perola (fall-winter, spring and summer, respectively), in no irrigated conditions. The experimental design was the randomized blocks in split plot design, with four replications. The parcels were represented by different soil tillage (conventional and no tillage), and the subparcels by doses of nitrogen applied in topdressing (0, 40, 80, 120, and 160 kg ha -1 of N); urea was used as source of N. Common bean crop has distinct responses to the doses of nitrogen in topdressing through the years of cultivation, with higher grain yield in the second year of the succession black oat/pearl millet/bean, needing, however, higher doses in no tillage system.

Prevalence of equine herpesvirus type 1 latency detected by polymerase chain reaction.

Abstract: In this study, an improved polymerase chain reaction (PCR) was used for detection of DNA of latent EHV-1 strains from several sources. Three pairs of oligonucleotide primers spanning fragments of 333 bp, 226 bp and 268 bp of the thymidine kinase (tk) gene, and one primer pair spanning 225 bp of the glycoprotein C (gC) gene were used in specific amplifications. Primers for EHV-4 PCR were also designed. Restriction digests with TaqI confirmed the identity of tk PCR fragments from EHV-1. The sensitivity to detect PCR products was further improved by visualisation in silver-stained acrylamide gels. PCR assays were applied to 267 samples including pools of tissue, peripheral blood leukocytes (PBL) and nasal swabs of archived, farms and abattoir specimens from a total of 116 animals. The EHV-1 DNA was found in 88% of the analysed samples. The prevalence of the EHV-1 latent or persistent form in adult horses was similar to others reports but found higher than previously described in foetuses and young foals. EHV-4 latency was not detected in the Brazilian studied specimens.