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Institution

Ministry of Agriculture

GovernmentRio de Janeiro, Brazil
About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Chemistry & Salmonella. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.


Papers
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Journal ArticleDOI
TL;DR: In this paper, a method for the determination of ten priority polycyclic aromatic hydrocarbons (PAHs) in roasted coffee by isotope dilution gas chromatography coupled to mass spectrometry was optimized, validated and applied to an exploratory study with 24 commercial samples.

30 citations

Journal ArticleDOI
TL;DR: Comparison of the antibody response in cattle elicited by vaccination with an attenuated ERA vaccine and an inactivated-adjuvanted PV vaccine shows no significant differences between the VNA titers and seropositivity rates obtained with IPVvac in the two methods tested.
Abstract: Despite the absence of current official reports showing the number of cattle infected by rabies, it is estimated that nearly 30,000 bovines are lost each year in Brazil. In order to minimize the important economic losses, control of the disease is achieved by eliminating bat colonies and by herd vaccination. In this study, we compare the antibody response in cattle elicited by vaccination with an attenuated ERA vaccine (AEvac) and an inactivated-adjuvanted PV (IPVvac) vaccine. The antibody titers were appraised by cell-culture neutralization test and ELISA, and the percentage of seropositivity was ascertained for a period of 180 days. IPVvac elicited complete seropositivity rates from day 30 to day 150, and even on day 180, 87% of the sera showed virus-neutralizing antibody titers (VNA) higher than 0.5IU/ml. There were no significant differences between the VNA titers and seropositivity rates obtained with IPVvac in the two methods tested. AEvac, however, elicited significantly lower titers than those observed in the group receiving inactivated vaccine. In addition, the profiles of antirabies IgG antibodies, evaluated by ELISA, and VNA, appraised by cell-culture neutralization test, were slightly different, when both vaccines were compared.

30 citations

Journal ArticleDOI
15 Sep 2016-PLOS ONE
TL;DR: The 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions, municipalities and farms, thus causing the spread of various M.bovis strains in herds from Midwest Brazil.
Abstract: Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goias. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRU-VNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = ≥ 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions, municipalities and farms, thus causing the spread of various M. bovis strains in herds from Midwest Brazil.

30 citations

Journal ArticleDOI
TL;DR: In this paper, the effects of sourdough fermentation on FODMAPs and organic acids were studied during the propagation and bread making of wheat bread, and the results showed that the higher level of organic acids and polyols, and lower content of fructans, sucrose, fructose and glucose than bread fermented by Saccharomyces cerevisiae.
Abstract: Fermentable, oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) are a class of carbohydrates poorly digested that may trigger the symptoms of Irritable Bowel Syndrome (IBS) and Non-Celiac Gluten Sensitivity (NCGS). The effects of sourdough fermentation on FODMAPs and organic acids were studied during the sourdough propagation and bread making. The concentrations of organic acids were higher for the first steps of propagation and became stable for final steps. All FODMAPs were significantly reduced during the propagation, except polyols. Sucrose, fructose and glucose were wholly degraded for the first step of fermentation. The other carbohydrates had their concentrations reduced after the fourth backslopping step. Sourdough bread presented the higher level of organic acids and polyols, and lower content of fructans, sucrose, fructose and glucose than bread fermented by Saccharomyces cerevisiae. The fructan reduction was from 69 to 75%, indicating that sourdough fermentation can be applied for producing low-FODMAP wheat bakery products.

30 citations


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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20238
202253
202157
202063
201951
201874