Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Chemistry & Salmonella. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Salmonella enterica mcr-1 Positive from Food in Brazil: Detection and Characterization

Abstract: The mcr-1 gene has been identified in bacterial isolates obtained from humans, animals, environment, and food, including Salmonella spp., which is one of the major foodborne pathogens worldwide. Th...

Validation of a quantitative and confirmatory method for residue analysis of aminoglycoside antibiotics in poultry, bovine, equine and swine kidney through liquid chromatography-tandem mass spectrometry

Abstract: The use of aminoglycoside antibiotics in food animals is approved in Brazil Accordingly, Brazilian food safety legislation sets maximum levels for these drugs in tissues from these animals in an effort to guarantee that food safety is not compromised Aiming to monitor the levels of these drugs in tissues from food animals, the validation of a quantitative, confirmatory method for the detection of residues of 10 aminoglycosides antibiotics in poultry, swine, equine and bovine kidney, with extraction using a solid phase and detection and quantification by LC-MS/MS was performed The procedure is an adaptation of the US Department of Agriculture, Food Safety and Inspection Service (USDA-FSIS) qualitative method, with the inclusion of additional clean-up and quantification at lower levels, which proved more efficient Extraction was performed using a phosphate buffer containing trifluoroacetic acid followed by neutralization, purification on a cationic exchange SPE cartridge, with elution with methanol/acetic acid, evaporation, and dilution in ion-pair solvent The method was validated according to the criteria and requirements of the European Commission Decision 2002/657/EC, showing selectivity with no matrix interference Linearity was established for all analytes using the method of weighted minimum squares CCα and CCβ varied between 1036 and 12,293 µg kg(-1), and between 1073 and 14,588 µg kg(-1), respectively The limits of quantification varied between 27 and 688 µg kg(-1) The values of recovery for all analytes in poultry kidney, fortified in the range of 500-1500 µg kg(-1), were higher than 90%, and the relative standard deviations were lower than 15%, except spectinomycin (218%) Uncertainty was estimated using a simplified methodology of 'bottom-up' and 'top-down' strategies The results showed that this method is effective for the quantification and confirmation of aminoglycoside residues and could be used by the Brazilian programme of residue control

Survey and characterization of root-knot nematode species in kiwi in Rio Grande do Sul State, Brazil

Abstract: Quarenta e quatro populacoes de Meloidogyne spp. provenientes de amostras de raizes de quivi, coletadas em pomares e viveiros do Estado do Rio Grande do Sul, Brasil, foram caracterizadas bioquimicamente utilizando-se a isoenzima esterase (Est). Meloidogyne arenaria Est A2 (Rm: 1,20, 1,28) foi a especie mais frequente e ocorreu em 66,65% das amostras. M. ethiopica com fenotipo Est E3 (Rm: 0,92, 1,10, 1,30) foi detectada em 16,66% das amostras, ocorrendo sempre em associacao com outras especies do genero. Tambem foram identificadas M. javanica Est J3 (Rm: 1,00, 1,21, 1,35), M. hapla Est H1(Rm: 1,17), M. incognita Est I1 (Rm: 1,03) e Est I2 (Rm: 1,03, 1,10) em 29,9%, 16,66%, 3,33% e 9,79% das amostras, respectivamente. Uma unica populacao atipica de Meloidogyne sp. (3,33%) com o fenotipo Est L3 (Rm: 1,00, 1,10, 1,30) foi detectada, porem nesse caso, nao foi possivel identificar a especie com seguranca, mesmo quando se recorreu a analise da configuracao perineal das femeas.