Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Biology & Chemistry. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Isolamento de Mycobacterium bovis em cão

Abstract: This report describes the isolation of Mycobacterium bovis from a dog with a history of co-habitation with bufallos infected with Mycobacterium bovis. After necropsy, the microrganism was isolated from a mesenteric lymphatic node in Stonebrink media and bacterial identification was confirmed by biochemical tests.

Detection of Vaccinia Virus in Milk: Evidence of a Systemic and Persistent Infection in Experimentally Infected Cows

Abstract: Bovine vaccinia (BV) is a zoonosis caused by Vaccinia virus (VACV), which affects lactating cows and milkers. VACV DNA and infectious particles have been detected in milk of naturally infected cows. However, the period and pattern of VACV shedding in milk is unknown, as is whether the presence of VACV in milk is due to a localized or a systemic infection. To address those questions, eight lactating cows were inoculated with VACV in previously scarified teats. The experiment was divided in two phases. In Phase 1, milk samples were collected daily for 33 days, and in Phase 2, four animals from the first phase were immunosuppressed. In both phases, milk was collected with a sterile catheter on even days and by hand milking on odd days. All animals showed typical BV lesions in the inoculated teats. All milk samples were subjected to nested polymerase chain reaction (PCR) and real-time quantitative PCR to detect VACV DNA. PCR-positive samples were subjected to virus isolation. VACV DNA was intermitten...

Sucker for drinking purposes

Abstract: A bottle teat of an elastic material includes a hollow suction nipple and a circumferential mounting edge for fixation to the bottle opening of a drinking bottle A hollow transition zone is disposed between the mounting edge and suction nipple as well as at least one drinking slot oriented in the longitudinal direction of the suction nipple and extending from the outside up to the inside of the suction nipple in an outwardly bulged external end region of the suction nipple The bottle teat further includes at least one ventilation valve with a valve slot which is extended from the outer wall of the transition zone up to the inner wall of the transition zone