Ministry of Agriculture

About: Ministry of Agriculture is a government organization based out in Rio de Janeiro, Brazil. It is known for research contribution in the topics: Chemistry & Salmonella. The organization has 1153 authors who have published 1189 publications receiving 14442 citations.

Genotipicação de Clostridium perfringens isolados de frangos de corte através da PCR múltipla

Abstract: Clostridium perfringens (Cp) is an anaerobic gram-positive bacterium which causes gaseous gangrene and enterotoxaemias in humans and domestic animals, besides being the primary cause of necrotic enteritis in poultry. Cp isolates were preliminary identified according to the lecithinase test on agar TSC-egg yolk, colony with double haemolysis in desfibrinated horse blood agar, Gram staining and biochemical tests. Cp isolates (171) were obtained from the intestinal content of broiler chickens sampled in a slaughterhouse in Para de Minas city, MG, Brazil. Cp was isolated in 62/125 (49.6%) strains from jejunum content and in 109/125 (87.2%) of ileum. Cp strains were classified into five toxigenic types (A-E), using multiplex PCR assay for genotyping of the principal and lethal toxins in the detection of genes coding for toxins alfa, beta, epsilon e iota, nomely genes cpa, cpb, etx e iA genes, beta2 toxin (cpb2) and enterotoxin (cpe). From a total of 62 Cp jejunum isolates obtained 42/62 (67.7%) were type A, 1/62 (1.6%) type A with the amplification of products for beta2 toxin gene (A/B2), 0/62 (0%) type B, 17/62 (27.4%) type C and 1/62 (1.6%) type D. A total of 109 ileum Cp isolates were obtained being 62/109 (56.9%) type A, 3/109 (2.7%) type A/B2 toxin gene, 1/62 (0.9%) type B, 38/109 (34.9%) type C, 1/109 (0.9%) type D. Cp A (60.8%) and Cp C (32.2%) toxigenic types were the most prevalent types in the analyzed intestinal contents of broiler chickens Cp A 104/171 (60.8%) and 55/171 (32.2%) toxigenic types which were the most prevalent types analyzed into two partes of the intestinal content of broiler chickens. Five (2.9%) out of 171 Cp isolates were not typified. The iota toxin (iA) and enterotoxin gene (cpe) codifying genes were not identified.

FREQUÊNCIA DO Vírus da Leucemia Felina (VLFe) em FELINOS DOMÉSTICOS (Felis catus) SEMIDOMICILIADOS NOS MUNICÍPIOS DE PELOTAS E RIO GRANDE

Abstract: Considering the importance of FeLV in the feline clinic, as well as the likely agent spread from a symptomatic or asymptomatic feline bearer, this work has as objective the study of the frequency of FeLV in felines residents in the cities of the Pelotas and Rio Grande, municipalities located in the south area of Brazil. For that, the blood of 120 semi-domiciled animals was collected for the detection of the retrovirus through the Indirect Immunofluorescence technique (IFA). FeLV was detected in 38,3% (46/120) of the studied animals, representing a larger frequency considering other studies accomplished in other areas of Brazil, what confirms the importance of FeLV in the studied region. KEY WORDS: FeLV, felines, immunofluorescence, retrovirus.

Isolamento de esporos de Paenibacillus larvae subsp. larvae no Brasil

Abstract: The objective of this work was to detect the presence of Paenibacillus larvae subsp. larvae spores in products from a warehouse located in Rio Grande do Sul State, Brazil, the identification of possible contamination sources, and the assessment of spores transference possibility from contaminated imported products from the warehouse to apiaries located in the surrounding area. Samples of imported pollen and bulk honey stocked in the warehouse, and honeycomb (brood, honey and pollen) from a healthy hive, honey from one apiary and adult bees were analyzed. Imported honey and pollen, and three groups of adult bees and the honey collected from the honeycomb resulted positive.

Efeito da adubação verde do feijoeiro "da sêca" com Crotalaria juncea L., empregando-se toda a vegetação ou retirando-se do campo as hastes despojadas de suas fôlhas

Abstract: Experiencias foram conduzidas, em diferentes localidades do Estado de Sao Paulo, para estudar o efeito da adubacao verde com Crotalaria juncea L., em cultura do feijoeiro "da seca", incorporando-se ao solo toda a vegetacao desta ou retirando-se do campo experimental as hastes despojadas de suas folhas. O aumento medio de producao proporcionado pela adubacao verde correspondeu a 41%, no primeiro caso, mas baixou para 28%, no segundo.

Outbreak of malignant oedema in sheep caused by Clostridium sordellii, predisposed by routine vaccination.

Abstract: J. L. N. Costa, DVM, MSc, PhD, M. M. D. Oliveira, DVM, Universidade Federal da Bahia, Salvador, Brazil F. C. F. Lobato, DVM, MSc, PhD, M. F. Souza Júnior, DVM, N. E. Martins, DVM, MSc, E. J. Facury Filho, DVM, MSc, PhD, V. L. V. Abreu, DVM, MSc, PhD, R. A. Assis, DVM, MSc, PhD, Laboratório de Anaeróbios da Escola de Veterinária da UFMG, Belo Horizonte, Minas Gerais, Brazil A.V.A. Carvalho, DVM, MSc, Ministério da Agricultura, Pecuária e Abastecimento, LARA-MG, Pedro Leopoldo, Minas Gerais, Brazil F. A. Uzal, DVM, MSc, PhD, DipACVP, California Animal Health and Food Safety Laboratory System, San Bernardino, 105 West Central Avenue, CA 92408,