Institution
Mitre Corporation
Company•Bedford, Massachusetts, United States•
About: Mitre Corporation is a company organization based out in Bedford, Massachusetts, United States. It is known for research contribution in the topics: Air traffic control & National Airspace System. The organization has 4884 authors who have published 6053 publications receiving 124808 citations. The organization is also known as: Mitre & MITRE.
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TL;DR: The isolation of an sdAb against Staphyloccocus aureus enterotoxin B (SEB) was found to have a high affinity and an extraordinarily high Tm and could still refold to recover activity after heat denaturation, making this sdAb a good candidate for use in antibody-based toxin detection technologies.
Abstract: Camelids and sharks possess a unique subclass of antibodies comprised of only heavy chains. The antigen binding fragments of these unique antibodies can be cloned and expressed as single domain antibodies (sdAbs). The ability of these small antigen-binding molecules to refold after heating to achieve their original structure, as well as their diminutive size, makes them attractive candidates for diagnostic assays. Here we describe the isolation of an sdAb against Staphyloccocus aureus enterotoxin B (SEB). The clone, A3, was found to have high affinity (Kd = 75 pM) and good specificity for SEB, showing no cross reactivity to related molecules such as Staphylococcal enterotoxin A (SEA), Staphylococcal enterotoxin D (SED), and Shiga toxin. Most remarkably, this anti-SEB sdAb had an extremely high Tm of 85°C and an ability to refold after heating to 95°C. The sharp Tm determined by circular dichroism, was found to contrast with the gradual decrease observed in intrinsic fluorescence. We demonstrated the utility of this sdAb as a capture and detector molecule in Luminex based assays providing limits of detection (LODs) of at least 64 pg/mL. The anti-SEB sdAb A3 was found to have a high affinity and an extraordinarily high Tm and could still refold to recover activity after heat denaturation. This combination of heat resilience and strong, specific binding make this sdAb a good candidate for use in antibody-based toxin detection technologies.
42 citations
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TL;DR: In this paper, a new algorithm is given for the two-dimensional translational containment problem: find translations for k polygons which place them inside a polygonal container without overlapping.
42 citations
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TL;DR: In this article, a new class of finite dimensional reproducing kernel spaces of m × 1 vector valued analytic functions on a fairly general domain Ω+ is introduced, where reproducing kernels of these spaces have a special form which is based on an m × m matrix valued function Θ, which is J unitary on the boundary of Ω+.
42 citations
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42 citations
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26 Nov 2001TL;DR: In this paper, the unscented transformation is extended to use extra test points beyond the minimum necessary to determine the second moments of a multivariate normal distribution, which can improve the estimated mean and variance of the transformed distribution when the transforming function of its derivatives have discontinuities.
Abstract: The unscented transformation is extended to use extra test points beyond the minimum necessary to determine the second moments of a multivariate normal distribution. The additional test points can improve the estimated mean and variance of the transformed distribution when the transforming function of its derivatives have discontinuities.
42 citations
Authors
Showing all 4896 results
Name | H-index | Papers | Citations |
---|---|---|---|
Sushil Jajodia | 101 | 664 | 35556 |
Myles R. Allen | 82 | 295 | 32668 |
Barbara Liskov | 76 | 204 | 25026 |
Alfred D. Steinberg | 74 | 295 | 20974 |
Peter T. Cummings | 69 | 521 | 18942 |
Vincent H. Crespi | 63 | 287 | 20347 |
Michael J. Pazzani | 62 | 183 | 28036 |
David Goldhaber-Gordon | 58 | 192 | 15709 |
Yeshaiahu Fainman | 57 | 648 | 14661 |
Jonathan Anderson | 57 | 195 | 10349 |
Limsoon Wong | 55 | 367 | 13524 |
Chris Clifton | 54 | 160 | 11501 |
Paul Ward | 52 | 408 | 12400 |
Richard M. Fujimoto | 52 | 290 | 13584 |
Bhavani Thuraisingham | 52 | 563 | 10562 |