Institution
Mitsubishi
Company•Tokyo, Japan•
About: Mitsubishi is a company organization based out in Tokyo, Japan. It is known for research contribution in the topics: Signal & Layer (electronics). The organization has 53115 authors who have published 54821 publications receiving 870150 citations. The organization is also known as: Mitsubishi Group of Companies & Mitsubishi Companies.
Topics: Signal, Layer (electronics), Semiconductor memory, Electrode, Voltage
Papers published on a yearly basis
Papers
More filters
••
TL;DR: Comparison of the sequence of another cDNA clone for a shorter form of hHGF mRNA revealed that the two sequences are identical in their 5' ends up to 865 nucleotides downstream from the translation-initiation site, then completely diverge from each other.
Abstract: We recently reported the isolation and sequencing of cDNA for human hepatocyte growth factor (hHGF) [Miyazawa, K., Tsubouchi, H., Naka, D., Takahashi, K., Okigaki, M., Arakaki, N., Nakayama, H., Hirono, S., Sakiyama, O., Takahashi, K., Gohda, E., Daikuhara, Y. & Kitamura, N. (1989) Biochem. Biophys. Res. Commun. 163, 967–973]. In the present study, we report the sequence of another cDNA clone for a shorter form of hHGF mRNA. Comparison of the sequence with that of the hHGF cDNA revealed that the two sequences are identical in their 5′ ends up to 865 nucleotides downstream from the translation-initiation site, then completely diverge from each other. By Northern blot analysis, the hHGF-related 1.5-kb mRNA, which corresponded to the newly isolated cDNA variant, was identified in human placenta. Sequence analysis of a human genomic HGF clone showed that the diverged 3′-terminal portion of the mRNA is generated by alternative RNA-processing events utilizing a specific exon. The mRNA could encode a short hHGF molecule of 290 amino acids corresponding to the N-terminal portion of hHGF which consists of 728 amino acids. In order to examine the effect of the predicted translation product on hepatocyte growth, an expression plasmid for the cDNA variant was constructed and transfected into Cos cells. Immunoblotting analysis showed that the transfected Cos cells produced a protein of about 33 kDa. The protein product did not stimulate DNA synthesis by rat hepatocytes in primary culture.
132 citations
••
TL;DR: The present results are consistent with the notion that age‐related methylation is associated with cancer susceptibility in the elderly and a promising method for risk assessment in the development of gastric cancer.
Abstract: A number of tumor suppressor and tumor-related genes are silenced by promoter hypermethylation in gastric cancer. Hypermethylation is not restricted to cancer cells, but is also present in non-neoplastic cells during aging. Such age-related methylation in non-neoplastic gastric epithelia is postulated to constitute a field defect that increases the risk for development of gastric cancer. To quantitatively evaluate age-related methylation in non-neoplastic gastric epithelia, we used a fiber-type DNA microarray on which methylated and unmethylated sequence probes were mounted. After bisulfite modification, a part of the promoter CpG island of four tumor suppressor genes, lysyl oxidase (LOX), p16, RUNX3 and tazarotene-induced gene 1 (TIG1), were amplified by PCR using Cy5 end labeled primers. Methylation rates (MRs) were calculated as the ratio of the fluorescence intensity of a methylated sequence probe to the total fluorescence intensity of methylated and unmethylated probes. Non-neoplastic gastric mucosa was obtained from 24 non-cancer-bearing stomachs at autopsy. MRs ranged from 0.0% to 77.2% (mean, 15.8%) for LOX, 0.0% to 45.8% (mean, 10.0%) for p16, 0.0% to 83.8% (mean, 9.0%) for RUNX3, and 0.0% to 46.1% (mean, 6.6%) for TIG1, and significantly correlated with aging (P < 0.01). The regression curves were: y = 0.013x(2) - 0.6184x + 4.0512, R(2) = 0.5728 (P < 0.001) for LOX; y = 0.0107x(2) - 0.6055x + 5.2943, R(2) = 0.7891 (P < 0.00001) for p16; y = 0.0182x(2) - 1.2234x + 11.566, R(2) = 0.5595 (P < 0.001) for RUNX3; and y = 0.0068 x(2) - 0.3586 x + 2.4306, R(2) = 0.4670 (P < 0.01) for TIG1. Thus, our present results are consistent with the notion that age-related methylation is associated with cancer susceptibility in the elderly. Quantitative analysis of DNA methylation using DNA microarrays is a promising method for risk assessment in the development of gastric cancer.
132 citations
••
TL;DR: The results indicate that missense mutations in this form of familial Alzheimer disease may act via a mechanism of impaired proteolytic processing of PS1.
132 citations
•
06 Jun 1979TL;DR: In this article, the authors proposed a process which allows a liquid to contact with the inside of a microporous hollow fiber and a fluid to contact on the outside, thereby allowing the gaseous components contained in both fluids to transfer between said liquid and said fluid.
Abstract: A process which comprises allowing a liquid to contact with the inside of a microporous hollow fiber and a fluid to contact with the outside, thereby allowing the gaseous components contained in both fluids to transfer between said liquid and said fluid. For use in the process, the inner diameter of the hollow fiber should be 50 to 5,000μ and the wall membrane should have an average micropore diameter of 0.01 to 0.5μ, a porosity of 10 vol.-% or more, a micropore surface area of 5 m2 /g or more, and an oxygen permeability of 10-6 cm3 (STP)·cm/cm2 ·sec·cmHg or more. In cases where the liquid is water or a liquid substance with aqueous medium, the process can be used effectively in artificial lung units or the like by using a hollow fiber of a polyolefin or a fluorinated polyolefin.
131 citations
••
TL;DR: The cloned and sequenced the leuB gene encoding 3-isopropylmalate dehydrogenase from Escherichia coli K-12 and the resulted mutant enzyme was more resistant to heat than the wild-type enzyme.
Abstract: We cloned and sequenced the leuB gene encoding 3-isopropylmalate dehydrogenase from Escherichia coli K-12 (JM103). Errors (33 residues) were found and corrected in the sequence previously reported for the leuB gene of Thermus thermophilus. The three-dimensional structure of the thermophile enzyme and the amino acid sequence comparison suggested that a part of the high stability of the T. thermophilus enzyme is conferred by increased hydrophobic interaction at the subunit-subunit interface. Two residues at the interface of the T. thermophilus enzyme, Leu246 and Val249, are substituted with less hydrophobic residues, Glu and Met, respectively, in the E. coli enzyme, whereas other residues in this region are highly conserved. The mutated T. thermophilus enzyme [L246E, V249M]IPMDH had reduced stability to heat. Two residues of the E. coli dehydrogenase, Glu256 and Met259, were replaced with the corresponding residues from the thermophile sequence. The resulted mutant enzyme was more resistant to heat than the wild-type enzyme.
131 citations
Authors
Showing all 53117 results
Name | H-index | Papers | Citations |
---|---|---|---|
Thomas S. Huang | 146 | 1299 | 101564 |
Kazunari Domen | 130 | 908 | 77964 |
Kozo Kaibuchi | 129 | 493 | 60461 |
Yoshimi Takai | 122 | 680 | 61478 |
William T. Freeman | 113 | 432 | 69007 |
Tadayuki Takahashi | 112 | 932 | 57501 |
Takashi Saito | 112 | 1041 | 52937 |
H. Vincent Poor | 109 | 2116 | 67723 |
Qi Tian | 96 | 1030 | 41010 |
Andreas F. Molisch | 96 | 777 | 47530 |
Takeshi Sakurai | 95 | 492 | 43221 |
Akira Kikuchi | 93 | 412 | 28893 |
Markus Gross | 91 | 588 | 32881 |
Eiichi Nakamura | 90 | 845 | 31632 |
Michael Wooldridge | 87 | 543 | 50675 |