Showing papers by "Monash University published in 2005"

Standards for ecologically successful river restoration

Abstract: Summary 1. Increasingly, river managers are turning from hard engineering solutions to ecologically based restoration activities in order to improve degraded waterways. River restoration projects aim to maintain or increase ecosystem goods and services while protecting downstream and coastal ecosystems. There is growing interest in applying river restoration techniques to solve environmental problems, yet little agreement exists on what constitutes a successful river restoration effort. 2. We propose five criteria for measuring success, with emphasis on an ecological perspective. First, the design of an ecological river restoration project should be based on a specified guiding image of a more dynamic, healthy river that could exist at the site. Secondly, the river’s ecological condition must be measurably improved. Thirdly, the river system must be more self-sustaining and resilient to external perturbations so that only minimal follow-up maintenance is needed. Fourthly, during the construction phase, no lasting harm should be inflicted on the ecosystem. Fifthly, both pre- and postassessment must be completed and data made publicly available. 3. Determining if these five criteria have been met for a particular project requires development of an assessment protocol. We suggest standards of evaluation for each of the five criteria and provide examples of suitable indicators. 4. Synthesis and applications . Billions of dollars are currently spent restoring streams and rivers, yet to date there are no agreed upon standards for what constitutes ecologically beneficial stream and river restoration. We propose five criteria that must be met for a river restoration project to be considered ecologically successful. It is critical that the broad restoration community, including funding agencies, practitioners and citizen restoration groups, adopt criteria for defining and assessing ecological success in restoration. Standards are needed because progress in the science and practice of river restoration has been hampered by the lack of agreed upon criteria for judging ecological success. Without well-accepted criteria that are ultimately supported by funding and implementing agencies, there is little incentive for practitioners to assess and report restoration outcomes. Improving methods and weighing the ecological benefits of various restoration approaches require organized national-level reporting systems.

Evidence and implications of recent climate change in northern Alaska and other arctic regions.

Abstract: The Arctic climate is changing. Permafrost is warming, hydrological processes are chang- ing and biological and social systems are also evolving in response to these changing conditions. Knowing how the structure and function of arctic terrestrial ecosystems are responding to recent and persistent climate change is paramount to understanding the future state of the Earth system and how humans will need to adapt. Our holistic review presents a broad array of evidence that illustrates con- vincingly; the Arctic is undergoing a system-wide response to an altered climatic state. New extreme and seasonal surface climatic conditions are being experienced, a range of biophysical states and pro- cesses influenced by the threshold and phase change of freezing point are being altered, hydrological and biogeochemical cycles are shifting, and more regularly human sub-systems are being affected. Importantly, the patterns, magnitude and mechanisms of change have sometimes been unpredictable or difficult to isolate due to compounding factors. In almost every discipline represented, we show

Psychological predictors of problem mobile phone use

Abstract: Mobile phone use is banned or illegal under certain circumstances and in some jurisdictions. Nevertheless, some people still use their mobile phones despite recognized safety concerns, legislation, and informal bans. Drawing potential predictors from the addiction literature, this study sought to predict usage and, specifically, problematic mobile phone use from extraversion, self-esteem, neuroticism, gender, and age. To measure problem use, the Mobile Phone Problem Use Scale was devised and validated as a reliable self-report instrument, against the Addiction Potential Scale and overall mobile phone usage levels. Problem use was a function of age, extraversion, and low self-esteem, but not neuroticism. As extraverts are more likely to take risks, and young drivers feature prominently in automobile accidents, this study supports community concerns about mobile phone use, and identifies groups that should be targeted in any intervention campaigns.

Mining data streams: a review

Abstract: The recent advances in hardware and software have enabled the capture of different measurements of data in a wide range of fields. These measurements are generated continuously and in a very high fluctuating data rates. Examples include sensor networks, web logs, and computer network traffic. The storage, querying and mining of such data sets are highly computationally challenging tasks. Mining data streams is concerned with extracting knowledge structures represented in models and patterns in non stopping streams of information. The research in data stream mining has gained a high attraction due to the importance of its applications and the increasing generation of streaming information. Applications of data stream analysis can vary from critical scientific and astronomical applications to important business and financial ones. Algorithms, systems and frameworks that address streaming challenges have been developed over the past three years. In this review paper, we present the state-of-the-art in this growing vital field.

Captivating company: dimensions of attractiveness in employer branding

Abstract: The internal marketing concept specifies that an organisation’s employees are its first market. Themes such as ‘internal advertising’ and ‘internal branding’ have recently entered the marketing lex...

Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production

Abstract: The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of recombinant proteins has become increasing popular in recent times. P. pastoris is easier to genetically manipulate and culture than mammalian cells and can be grown to high cell densities. Equally important, P. pastoris is also a eukaryote, and thereby provides the potential for producing soluble, correctly folded recombinant proteins that have undergone all the post-translational modifications required for functionality. Additionally, linearized foreign DNA can be inserted in high efficiency via homologous recombination procedures to generate stable cell lines whilst expression vectors can be readily prepared that allow multiple copies of the target protein, multimeric proteins with different subunit structures, or alternatively the target protein and its cognate binding partners, to be expressed. A further benefit of the P. pastoris system is that strong promoters are available to drive the expression of a foreign gene(s) of interest, thus enabling production of large amounts of the target protein(s) with relative technical ease and at a lower cost than most other eukaryotic systems. The purpose of this review is to summarize important developments and features of this expression system and, in particular, to examine from an experimental perspective the genetic engineering, protein chemical and molecular design considerations that have to be taken into account for the successful expression of the target recombinant protein. Included in these considerations are the influences of P. pastoris strain selection; the choice of expression vectors and promoters; procedures for the transformation and integration of the vectors into the P. pastoris genome; the consequences of rare codon usage and truncated transcripts; and techniques employed to achieve multi-copy integration numbers. The impact of the alcohol oxidase (AOX) pathways in terms of the mut+ and muts phenotypes, intracellular expression and folding pathways is examined. The roles of pre–pro signal sequences such as the alpha mating factor (α-MF) and the Glu–Ala repeats at the kex2p cleavage site on the processing of the protein translate(s) have also been considered. Protocols for the generation of protein variants and mutants for screening for orphan cognate binding partners and the use of experimental platforms addressing the molecular recognition behaviour of recombinant proteins such as the extracellular domains of transmembrane receptors with their physiological ligands are also described. Finally, the palindromic patterns of glycosylation that can occur with these expression systems, in terms of the role and location of the sequon in the primary structure, the number of mannose units and the types of oligosaccharides incorporated as Asn- or O-linkages and their impact on the thermostability and immunogenicity of the recombinant protein are considered. Procedures to prevent glycosylation through manipulation of cell culture conditions or via enzymatic and site-directed mutagenesis methods are also discussed. Copyright © 2004 John Wiley & Sons, Ltd.

Travel Anxiety and Intentions to Travel Internationally: Implications of Travel Risk Perception:

Abstract: This article investigates the impact of cultural and psychographic factors on perceptions of travel risk, anxiety, and intentions to travel internationally. The study involved 246 Australian and 336 foreign respondents who were surveyed as to their cultural orientation, personality, lifestyle, travel motivation, risk and safety perception, anxiety, and intentions to travel. The results of a path analysis showed that the travel risk perception was a function of cultural orientation and psychographic factors in both samples, and anxiety was a function of type of perceived risk. The terrorism and sociocultural risk emerged as the most significant predictors of travel anxiety. Intentions to travel internationally were determined by travel anxiety levels and level of perceived safety. Implications for future research and marketing practices are discussed.

Developing a Pedagogy of Teacher Education: Understanding Teaching & Learning about Teaching

Abstract: 1. Developing a Pedagogy of Teacher Education: What Does that Really Mean? Section 1: Teaching About Teaching 2.Being a Teacher Educator: A Focus on Pedagogy 3. Teaching: A Problematic Enterprise 4. Making the Tacit Explicit 5.A Shared Language: Conceptualising Knowledge for a Pedagogy of Teacher Education 6. Principles of Practice Section 2: Learning about Teaching 7. Being a Student of Teaching 8. From Student to Teacher: The Place of Effective Reflective Practice 9. Student Teacher as Researcher: Recognizing and Valuing the Development of Professional Knowledge 10. Learning Through Experience: Students of Teaching Researching their Own Practice 11. Teacher Education as a Beginning Not an End 12. Enacting a Pedagogy of Teacher Education

Not so naive Bayes: aggregating one-dependence estimators

Abstract: Of numerous proposals to improve the accuracy of naive Bayes by weakening its attribute independence assumption, both LBR and Super-Parent TAN have demonstrated remarkable error performance. However, both techniques obtain this outcome at a considerable computational cost. We present a new approach to weakening the attribute independence assumption by averaging all of a constrained class of classifiers. In extensive experiments this technique delivers comparable prediction accuracy to LBR and Super-Parent TAN with substantially improved computational efficiency at test time relative to the former and at training time relative to the latter. The new algorithm is shown to have low variance and is suited to incremental learning.

Histone H3 serine 10 phosphorylation by Aurora B causes HP1 dissociation from heterochromatin

Abstract: Histones are subject to numerous post-translational modifications. Some of these 'epigenetic' marks recruit proteins that modulate chromatin structure. For example, heterochromatin protein 1 (HP1) binds to histone H3 when its lysine 9 residue has been tri-methylated by the methyltransferase Suv39h (refs 2-6). During mitosis, H3 is also phosphorylated by the kinase Aurora B. Although H3 phosphorylation is a hallmark of mitosis, its function remains mysterious. It has been proposed that histone phosphorylation controls the binding of proteins to chromatin, but any such mechanisms are unknown. Here we show that antibodies against mitotic chromosomal antigens that are associated with human autoimmune diseases specifically recognize H3 molecules that are modified by both tri-methylation of lysine 9 and phosphorylation of serine 10 (H3K9me3S10ph). The generation of H3K9me3S10ph depends on Suv39h and Aurora B, and occurs at pericentric heterochromatin during mitosis in different eukaryotes. Most HP1 typically dissociates from chromosomes during mitosis, but if phosphorylation of H3 serine 10 is inhibited, HP1 remains chromosome-bound throughout mitosis. H3 phosphorylation by Aurora B is therefore part of a 'methyl/phos switch' mechanism that displaces HP1 and perhaps other proteins from mitotic heterochromatin.

Towards safe, non-viral therapeutic gene expression in humans

Abstract: The potential dangers of using viruses to deliver and integrate DNA into host cells in gene therapy have been poignantly highlighted in recent clinical trials. Safer, non-viral gene delivery approaches have been largely ignored in the past because of their inefficient delivery and the resulting transient transgene expression. However, recent advances indicate that efficient, long-term gene expression can be achieved by non-viral means. In particular, integration of DNA can be targeted to specific genomic sites without deleterious consequences and it is possible to maintain transgenes as small episomal plasmids or artificial chromosomes. The application of these approaches to human gene therapy is gradually becoming a reality.

PI 3-kinase p110β: a new target for antithrombotic therapy

Abstract: Platelet activation at sites of vascular injury is essential for the arrest of bleeding; however, excessive platelet accumulation at regions of atherosclerotic plaque rupture can result in the development of arterial thrombi, precipitating diseases such as acute myocardial infarction and ischemic stroke. Rheological disturbances (high shear stress) have an important role in promoting arterial thrombosis by enhancing the adhesive and signaling function of platelet integrin alpha(IIb)beta(3) (GPIIb-IIIa). In this study we have defined a key role for the Type Ia phosphoinositide 3-kinase (PI3K) p110beta isoform in regulating the formation and stability of integrin alpha(IIb)beta(3) adhesion bonds, necessary for shear activation of platelets. Isoform-selective PI3K p110beta inhibitors have been developed which prevent formation of stable integrin alpha(IIb)beta(3) adhesion contacts, leading to defective platelet thrombus formation. In vivo, these inhibitors eliminate occlusive thrombus formation but do not prolong bleeding time. These studies define PI3K p110beta as an important new target for antithrombotic therapy.

A critical analysis of decision support systems research

Abstract: This paper critically analyses the nature and state of decision support systems (DSS) research. To provide context for the analysis, a history of DSS is presented which focuses on the evolution of a number of sub-groupings of research and practice: personal DSS, group support systems, negotiation support systems, intelligent DSS, knowledge management-based DSS, executive information systems/business intelligence, and data warehousing. To understand the state of DSS research an empirical investigation of published DSS research is presented. This investigation is based on the detailed analysis of 1,020 DSS articles published in 14 major journals from 1990 to 2003. The analysis found that DSS publication has been falling steadily since its peak in 1994 and the current publication rate is at early 1990s levels. Other findings include that personal DSS and group support systems dominate research activity and data warehousing is the least published type of DSS. The journal DSS is the major publishing outlet; US ‘Other’ journals dominate DSS publishing and there is very low exposure of DSS in European journals. Around two-thirds of DSS research is empirical, a much higher proportion than general IS research. DSS empirical research is overwhelming positivist, and is more dominated by positivism than IS research in general. Design science is a major DSS research category. The decision support focus of the sample shows a well-balanced mix of development, technology, process, and outcome studies. Almost half of DSS papers did not use judgement and decision-making reference research in the design and analysis of their projects and most cited reference works are relatively old. A major omission in DSS scholarship is the poor identification of the clients and users of the various DSS applications that are the focus of investigation.

The Grid Economy

Abstract: This work identifies challenges in managing resources in a Grid computing environment and proposes computational economy as a metaphor for effective management of resources and application scheduling. It identifies distributed resource management challenges and requirements of economy-based Grid systems, and discusses various representative economy-based systems, both historical and emerging, for cooperative and competitive trading of resources such as CPU cycles, storage, and network bandwidth. It presents an extensive, service-oriented Grid architecture driven by Grid economy and an approach for its realization by leveraging various existing Grid technologies. It also presents commodity and auction models for resource allocation. The use of commodity economy model for resource management and application scheduling in both computational and data grids is also presented.

Testosterone Patch Increases Sexual Activity and Desire in Surgically Menopausal Women with Hypoactive Sexual Desire Disorder

Abstract: Context: Hypoactive sexual desire disorder (HSDD) is one of the most common sexual problems reported by women, but few studies have been conducted to evaluate treatments for this condition. Objective: The objective of this study was to evaluate the efficacy and safety of a testosterone patch in surgically menopausal women with HSDD. Design: The design was a randomized, double-blind, parallel-group, placebo-controlled, 24-wk study (the Intimate SM 1 study). Setting: The study was performed at private or institutional practices. Patients: The subjects studied were women, aged 26–70 yr, with HSDD after bilateral salpingo-oophorectomy who were receiving concomitant estrogen therapy. Placebo (n = 279) or testosterone 300 μg/d (n = 283) was administered. There were 19 patients who withdrew due to adverse events in the placebo group and 24 in the 300 μg/d testosterone group. Intervention: Testosterone (300 μg/d) or placebo patches were applied twice weekly. Main Outcome Measure(s): The primary end point was the ...

Activation of Thymic Regeneration in Mice and Humans following Androgen Blockade

Abstract: The thymus undergoes age-related atrophy, coincident with increased circulating sex steroids from puberty. The impact of thymic atrophy is most profound in clinical conditions that cause a severe loss in peripheral T cells with the ability to regenerate adequate numbers of naive CD4+ T cells indirectly correlating with patient age. The present study demonstrates that androgen ablation results in the complete regeneration of the aged male mouse thymus, restoration of peripheral T cell phenotype and function and enhanced thymus regeneration following bone marrow transplantation. Importantly, this technique is also applicable to humans, with analysis of elderly males undergoing sex steroid ablation therapy for prostatic carcinoma, demonstrating an increase in circulating T cell numbers, particularly naive (TREC+) T cells. Collectively these studies represent a fundamentally new approach to treating immunodeficiency states in humans.

The relationship between total quality management practices and organizational culture

Abstract: Purpose – This empirical study explores the relationship between total quality management (TQM) practices and organizational culture with the purpose of identifying the particular cultures that determine the successful implementation of TQM practices. Specifically, it tests two competing views on the relationship; the unitarist and pluralist views.Design/methodology/approach – The empirical data was drawn from 194 organizations in Australia. The research model employs the Malcolm Baldrige National Quality Award criteria as TQM framework and builds on the competing values model to frame organizational culture. The data was analysed using structural equation modelling technique.Findings – The findings support the pluralist view, wherein different subsets of TQM practices are determined by different types of cultures. Interestingly, hierarchical culture was found to have a significant relationship with certain practices of TQM. Additionally, the findings indicate that although the cultural factors underpinni...

Person-job fit versus person-organization fit as predictors of organizational attraction and job acceptance intentions : A longitudinal study

Abstract: This longitudinal field study examined the relationship between perceived person‐job (PJ) and person‐organization (PO) fit and organizational attraction, intentions to accept a job offer, and actual job offer decision. Data were collected from 193 graduate applicants prior to the selection process, during the selection process, at the end of the selection process, and after job acceptance decision. The findings showed support for the hypothesis that perceptions of PJ and PO fit influenced attraction at different stages of selection. The second hypothesis that the relationship between perceptions of PJ and PO fit and intentions to accept a job offer are mediated by organizational attraction was partially supported. Mid-selection, the relationship between PJ fit perceptions and intentions to accept a job offer was mediated by organizational attraction; in contrast, at the end of the selection process, there was a direct relationship between PJ fit perceptions and intentions. PO fit perceptions were unrelated to intentions to accept a job offer. PJ and PO fit perceptions (before and during the selection process) were unrelated to actual job acceptance decision. These findings highlight the importance of ensuring that applicants have sufficient information about the job during the recruitment and selection process.

Combining quantitative and qualitative measures of uncertainty in model-based environmental assessment: the NUSAP system.

Abstract: This article discusses recent experiences with the Numeral Unit Spread Assessment Pedigree (NUSAP) system for multidimensional uncertainty assessment, based on four case studies that vary in complexity. We show that the NUSAP method is applicable not only to relatively simple calculation schemes but also to complex models in a meaningful way and that NUSAP is useful to assess not only parameter uncertainty but also (model) assumptions. A diagnostic diagram can be used to synthesize results of quantitative analysis of parameter sensitivity and qualitative review (pedigree analysis) of parameter strength. It provides an analytic tool to prioritize uncertainties according to quantitative and qualitative insights in the limitations of available knowledge. We show that extension of the pedigree scheme to include societal dimensions of uncertainty, such as problem framing and value-laden assumptions, further promotes reflexivity and collective learning. When used in a deliberative setting, NUSAP pedigree assessment has the potential to foster a deeper social debate and a negotiated management of complex environmental problems.

Language Difficulties of International Students in Australia: The Effects of Prior Learning Experience.

Abstract: Globalisation has placed a growing importance on English language speaking and listening. Prior research indicates that many international students from Asia, studying in Australia, face serious learning difficulties and lack confidence in speaking and taking a proactive role in classrooms. The paper reports on data gathered in interviews with students from five Asian nations, which suggest that these learning difficulties are grounded in weaknesses in students’ prior learning experiences – focused on grammar and reading skills in teacher-centred classrooms, not conversational skills – and in beliefs about language learning instilled during schooling. The paper proposes strategies for overcoming these problems.