Showing papers by "Moscow State University published in 2002"
767 citations
TL;DR: The lead-lead isochron age of chondrules in the CR chondrite Acfer 059 is 4564.7 ± 0.6 million years ago, which indicates that CAI- and chondrule-forming events lasted for at least 1.3 My and supports the chronological significance of the 26Al-26Mg systematics.
Abstract: The lead-lead isochron age of chondrules in the CR chondrite Acfer 059 is 4564.7 ± 0.6 million years ago (Ma), whereas the lead isotopic age of calcium-aluminum–rich inclusions (CAIs) in the CV chondrite Efremovka is 4567.2 ± 0.6 Ma. This gives an interval of 2.5 ± 1.2 million years (My) between formation of the CV CAIs and the CR chondrules and indicates that CAI- and chondrule-forming events lasted for at least 1.3 My. This time interval is consistent with a 2- to 3-My age difference between CR CAIs and chondrules inferred from the differences in their initial26Al/27Al ratios and supports the chronological significance of the 26Al-26Mg systematics.
747 citations
TL;DR: In this article, a code that calculates the relic density of the lightest supersymmetric particle (LSP) in the minimal supersymmymmetric standard model is presented, where all tree level processes for the annihilation of the LSP are included as well as all possible coannihilation processes with neutralinos, charginos, sleptons, squarks and gluinos.
642 citations
TL;DR: Spectral reflectance of maple, chestnut and beech leaves in a wide range of pigment content and composition was investigated to devise a nondestructive technique for total carotenoid (Car) content estimation in higher plant leaves.
Abstract: Spectral reflectance of maple, chestnut and beech leaves in a wide range of pigment content and composition was investigated to devise a nondestructive technique for total carotenoid (Car) content estimation in higher plant leaves. Reciprocal reflectance in the range 510 to 550 nm was found to be closely related to the total pigment content in leaves. The sensitivity of reciprocal reflectance to Car content was maximal in a spectral range around 510 nm; however, chlorophylls (Chl) also affect reflectance in this spectral range. To remove the Chl effect on the reciprocal reflectance at 510 nm, a reciprocal reflectance at either 550 or 700 nm was used, which was linearly proportional to the Chl content. Indices for nondestructive estimation of Car content in leaves were devised and validated. Reflectances in three spectral bands, 510+/-5 nm, either 550+/-15 nm or 700+/-7.5 nm and the near infrared range above 750 nm are sufficient to estimate total Car content in plant leaves nondestructively with a root mean square error of less than 1.75 nmol/cm2.
620 citations
TL;DR: It is demonstrated that two rhodopsins, identified from cDNA sequences, function as low- and high-light-intensity phototaxis receptors in the eukaryotic alga Chlamydomonas reinhardtii by in vivo analysis of photoreceptor electrical currents and motility responses in transformants with RNA interference directed against each of the r Rhodopsin genes.
Abstract: We demonstrate that two rhodopsins, identified from cDNA sequences, function as low- and high-light-intensity phototaxis receptors in the eukaryotic alga Chlamydomonas reinhardtii. Each of the receptors consists of an ≈300-residue seven-transmembrane helix domain with a retinal-binding pocket homologous to that of archaeal rhodopsins, followed by ≈400 residues of additional membrane-associated portion. The function of the two rhodopsins, Chlamydomonas sensory rhodopsins A and B (CSRA and CSRB), as phototaxis receptors is demonstrated by in vivo analysis of photoreceptor electrical currents and motility responses in transformants with RNA interference (RNAi) directed against each of the rhodopsin genes. The kinetics, fluence dependencies, and action spectra of the photoreceptor currents differ greatly in transformants in accord with the relative amounts of photoreceptor pigments expressed. The data show that CSRA has an absorption maximum near 510 nm and mediates a fast photoreceptor current that saturates at high light intensity. In contrast, CSRB absorbs maximally at 470 nm and generates a slow photoreceptor current saturating at low light intensity. The relative wavelength dependence of CSRA and CSRB activity in producing phototaxis responses matches precisely the wavelength dependence of the CSRA- and CSRB-generated currents, demonstrating that each receptor mediates phototaxis. The saturation of the two photoreceptor currents at different light fluence levels extends the range of light intensity to which the organism can respond. Further, at intensities where both operate, their light signals are integrated at the level of membrane depolarization caused by the two photoreceptor currents.
582 citations
09 Apr 2002
TL;DR: Polytopes topology and combinatorics of simplicial complexes Commutative and homological algebra of Cubical complexes Cubical Complexes Toric and quasitoric manifolds Moment-angle Complexes Cohomology of moment-angle complexes as discussed by the authors.
Abstract: Introduction Polytopes Topology and combinatorics of simplicial complexes Commutative and homological algebra of simplicial complexes Cubical complexes Toric and quasitoric manifolds Moment-angle complexes Cohomology of moment-angle complexes and combinatorics of triangulated manifolds Cohomology rings of subspace arrangement complements Bibliography Index.
547 citations
TL;DR: The results establish EPO as an important paracrine neuroprotective mediator of ischemic preconditioning and provide evidence for the following signaling cascade: HIF-1 is activated rapidly by hypoxia in astrocytes and EPO activates the neuronal EPO receptor and, subsequently, JAK-2 and thereby PI3K.
Abstract: In an in vitro model of cerebral ischemia (oxygen glucose deprivation, OGD) we investigated whether erythropoietin (EPO) plays a critical role in ischemic preconditioning. We found that EPO time and dose-dependently induced protection against OGD in rat primary cortical neurons. Protection was significant at 5 min and reached a maximum at 48 hr after EPO application. Protection was blocked by the coapplication of a soluble Epo receptor (sEpoR) or an antibody against EpoR (anti-EpoR). Medium transfer from OGD-treated astrocytes to untreated neurons induced protection against OGD in neurons, which was attenuated strongly by the application of sEpoR and anti-EpoR. In contrast, medium transfer from OGD-treated neurons to untreated neurons induced protection against OGD that did not involve EPO. In astrocytes the OGD enhanced the nuclear translocation of hypoxia-inducible factor 1 (HIF-1), the major transcription factor regulating EPO expression. Consequently, transcription of EPO-mRNA was increased in astrocytes after OGD. Cultured neurons express EpoR, and the Janus kinase-2 (JAK-2) inhibitor AG490 abolished EPO-induced tolerance against OGD. Furthermore, EPO-induced neuroprotection as well as phosphorylation of the proapoptotic Bcl family member Bad was reduced by the phosphoinositide-3 kinase (PI3K) inhibitor LY294002. The results suggest that astrocytes challenged with OGD provide paracrine protective signals to neurons. We provide evidence for the following signaling cascade: HIF-1 is activated rapidly by hypoxia in astrocytes. After HIF-1 activation the astrocytes express and release EPO. EPO activates the neuronal EPO receptor and, subsequently, JAK-2 and thereby PI3K. PI3K deactivates BAD via Akt-mediated phosphorylation and thus may inhibit hypoxia-induced apoptosis in neurons. Our results establish EPO as an important paracrine neuroprotective mediator of ischemic preconditioning.
475 citations
TL;DR: In this article, the authors used the propagation code GALPROP to calculate interstellar cosmic-ray propagation for a variety of models and showed that there is no simple model capable of accurately describing the whole variety of data: boron/carbon and sub-iron/iron ratios, spectra of protons, helium, antiprotons, positrons, electrons, and diUuse c-rays.
Abstract: High-energy collisions of cosmic-ray nuclei with interstellar gas are believed to be the mechanism producing the majority of cosmic-ray antiprotons. Because of the kinematics of the process, they are created with a nonzero momentum; the characteristic spectral shape with a maximum at D2 GeV and a sharp decrease toward lower energies makes antiprotons a unique probe of models for particle propagation in the Galaxy and modulation in the heliosphere. On the other hand, accurate calculation of the secondary antiproton —ux provides a ii background ˇˇ for searches for exotic signals from the annihilation of supersymmetric particles and primordial black hole evaporation. Recently, new data with large statistics on both low- and high-energy antiproton —uxes have become available which allow such tests to be performed. We use our propagation code GALPROP to calculate interstellar cosmic-ray propagation for a variety of models. We show that there is no simple model capable of accurately describing the whole variety of data: boron/carbon and sub-iron/iron ratios, spectra of protons, helium, antiprotons, positrons, electrons, and diUuse c-rays. We —nd that only a model with a break in the diUusion coefficient plus convection can reproduce measurements of cosmic-ray species, and the reproduction of primaries (p, He) can be further improved by introducing a break in the primary injection spectra. For our best-—t model we make predictions of proton and antiproton —uxes near the Earth for diUerent modulation levels and magnetic polarity using a steady state drift model of propagation in the heliosphere.
457 citations
TL;DR: A new class of nonlinear acoustic phenomena has been observed for acoustic wave interaction with simulated and realistic nonbonded contact interfaces (cracked defects) in solids.
373 citations
TL;DR: The buffer composition was shown to have a dramatic effect on the assay stability, where the commonly used phosphate buffer saline was proved to be the least suitable choice.
Abstract: Silicon microchips with immobilized antibodies were used to develop microfluidic enzyme immunoassays using chemiluminescence detection and horseradish peroxidase (HRP) as the enzyme label. Polyclonal anti-atrazine antibodies were coupled to the silicon microchip surface with an overall dimension of 13.1 x 3.2 mm, comprising 42 porous flow channels of 235-microm depth and 25-microm width. Different immobilization protocols based on covalent or noncovalent modification of the silica surface with 3-aminopropyltriethoxysilane (APTES) or 3-glycidoxypropyltrimethoxysilane (GOPS), linear polyethylenimine (LPEI, MW 750,000), or branched polyethylenimine (BPEI, MW 25,000), followed by adsorption or covalent attachment of the antibody, were evaluated to reach the best reusability, stability, and sensitivity of the microfluidic enzyme immunoassay (microFEIA). Adsorption of antibodies on a LPEI-modified silica surface and covalent attachment to physically adsorbed BPEI lead to unstable antibody coatings. Covalent coupling of antibodies via glutaraldehyde (GA) to three different functionalized silica surfaces (APTES-GA, LPEI-GA, and GOPS-BPEI-GA) resulted in antibody coatings that could be completely regenerated using 0.4 M glycine/HCl, pH 2.2. The buffer composition was shown to have a dramatic effect on the assay stability, where the commonly used phosphate buffer saline was proved to be the least suitable choice. The best long-term stability was obtained for the LPEI-GA surface with no loss of antibody activity during one month. The detection limits in the microFEIA for the three different immuno surfaces were 45, 3.8, and 0.80 ng/L (209, 17.7, and 3.7 pM) for APTES-GA, LPEI-GA, and GOPS-BPEI-GA, respectively.
316 citations
TL;DR: The Porcupine Basin, southwest of Ireland, was one of the earliest sites from where the deep-water corals Lophelia sp. and Madrepora sp. were recovered as mentioned in this paper.
TL;DR: In this article, a numerical model of river morphology for meander bends with erodible cohesive banks was developed and tested, where the governing conservation equations were implemented in a moving boundary fitted coordinate system that can be both curvilinear and nonorthogonal.
Abstract: A numerical model of river morphology for meander bends with erodible cohesive banks is herein developed and tested. The new model has three key features. First, it couples a two-dimensional depth-averaged model of flow and bed topography with a mechanistic model of bank erosion. Second, it simulates the deposition of failed bank material debris at and its subsequent removal from the toe of the bank. Finally, the governing conservation equations are implemented in a moving boundary fitted coordinate system that can be both curvilinear and nonorthogonal. This simplifies grid generation in curved channels that experience bank deformation, allowing complex planform shapes associated with irregular natural channels to be simulated. Model performance is assessed using data from two flume experiments and a natural river channel. Results are encouraging, but the model underpredicts the scour depth in pools adjacent to the outer bank and, consequently, underpredicts bank migration rates.
University of Bari1, Moscow State University2, Institut national de la recherche agronomique3, Imperial College London4, Oregon State University5, University of California, Davis6, United States Department of Agriculture7, Thomas Jefferson University8, University of Tokyo9, University of Florida10, Iwate University11
TL;DR: The mealybug-transmitted species have been separated from the genus Closterovirus and accommodated in a new genus named Ampelovirus (from ampelos, Greek for grapevine), and the family now comprises three genera.
Abstract: Recently obtained molecular and biological information has prompted the revision of the taxonomic structure of the family Closteroviridae. In particular, mealybug-transmitted species have been separated from the genus Closterovirus and accommodated in a new genus named Ampelovirus (from ampelos, Greek for grapevine). Thus, the family now comprises three genera. Their major properties are (i) Closterovirus: type species Beet yellows virus, genome monopartite, 15.5–19.3 kb in size, a 22–25 kDa major coat protein (CP), the gene encoding the divergent CP analogue (CPd) upstream of the CP cistron, transmission by aphids, a membership of 8 definitive and 4 tentative species; (ii) Ampelo-virus: type species Grapevine leafroll virus 3, genome monopartite 16.9–19.5 kb in size, a 35–37 kDa major CP, a CPd cistron generally located downstream of the CP gene, transmission by pseudococcid and coccid mealybugs, a membership of 6 definitive and 5 tentative species; (iii) Crinivirus: type species Lettuce infectious yellows virus, genome essentially bipartite 15.3–19 kb in size, a 28–33 kDa CP, a CPd cistron downstream of the CP gene, transmission by whiteflies (Bemisia, Trialeurodes), a membership of 7 definitive and 3 tentative species. There are five unassigned species in the family.
TL;DR: In this paper, time dependent solutions of supergravities with dilaton and arbitrary rank antisymmetric tensor field are found, which are related to Euclidean or spacelike branes.
Abstract: In this paper time dependent solutions of supergravities with dilaton and arbitrary rank antisymmetric tensor field are found. Although the solutions are nonsupersymmetric the equations of motions can be integrated in a simple form. Such supergravity solutions are related to Euclidean or spacelike branes (S-branes).
Ohio State University1, Harvard University2, University of Arizona3, Moscow State University4, Russian Academy of Sciences5, Special Astrophysical Observatory6, National Institute of Astrophysics, Optics and Electronics7, University of California, Berkeley8, University of Florida9, Sternberg Astronomical Institute10, Academy of Sciences of Uzbekistan11, University of Massachusetts Amherst12, Shanghai Astronomical Observatory13
TL;DR: In this paper, the authors present the final installment of an intensive 13-year study of variations of the optical continuum and broad H beta emission line in the Seyfert 1 galaxy NGC 5548.
Abstract: We present the final installment of an intensive 13 year study of variations of the optical continuum and broad H beta emission line in the Seyfert 1 galaxy NGC 5548. The database consists of 1530 optical continuum measurements and 1248 H beta measurements. The H beta variations follow the continuum variations closely, with a typical time delay of about 20 days. However, a year-by-year analysis shows that the magnitude of emission-line time delay is correlated with the mean continuum flux. We argue that the data are consistent with the simple model prediction between the size of the broad-line region and the ionizing luminosity, r is proportional to L(sup 1/2)(sub ion). Moreover, the apparently linear nature of the correlation between the H beta response time and the nonstellar optical continuum F(sub opt) arises as a consequence of the changing shape of the continuum as it varies, specifically F(sub opt) is proportional to F(sup 0.56)(sub UV).
TL;DR: It is suggested that persistent growth and asymmetric transition frequencies serve the biological function of providing a mechanism by which microtubules may rapidly accommodate to the changing shape and advancing edge of motile cells.
Abstract: Microtubule dynamics were investigated in CHO and NRK cells by novel experimental approaches designed to evaluate the microtubule behavior in the cell interior. These approaches were: (1) laser photobleaching of a path through the centrosome; (2) direct observation of microtubules in centrosome-containing cytoplasts; (3) GFP-CLIP-170 expression as a marker for microtubule plus end growth; and (iv) sequential subtraction analysis. The combination of these approaches allowed us to obtain data where the density of microtubules had previously prevented conventional methods to be applicable. In the steady state, nascent microtubules grew persistently from the centrosome towards the cell margin. Frequently, they arrived at the cell margin without undergoing any transition to the shortening phase. In contrast to the growth of microtubules, shortening of the plus ends from the periphery was non-persistent; that is, rescue was frequent and the extent of shortening showed a distribution of lengths reflecting a stochastic process. The combination of persistent growth and a cell boundary led to a difference in apparent microtubule behavior in the cell interior compared with that near the cell margin. Whereas microtubules in the cell interior showed asymmetric transition frequencies, their behavior near the cell margin showed frequent fluctuations between phases of shortening and growth. Complete microtubule turnover was accomplished by the relatively rare episodes of shortening back to the centrosome. Release from the centrosome with subsequent minus end shortening also occurred but was a minor mechanism for microtubule turnover compared with the plus end pathway. We propose a life cycle for a microtubule which consists of rapid growth from the centrosome to the cell margin followed by an indefinite period of fluctuations of phases of shortening and growth. We suggest that persistent growth and asymmetric transition frequencies serve the biological function of providing a mechanism by which microtubules may rapidly accommodate to the changing shape and advancing edge of motile cells.
TL;DR: In this article, the main aspects of nonequilibrium hot-electron phenomena in superconductors and various theoretical models developed to describe the hotelectron effect are discussed, and the most successful practical devices such as terahertz mixers and direct intensity detectors for far-infrared radiation are presented.
Abstract: The paper reviews the main aspects of nonequilibrium hot-electron phenomena in superconductors and various theoretical models developed to describe the hot-electron effect. We discuss implementation of the hot-electron avalanche mechanism in superconducting radiation sensors and present the most successful practical devices, such as terahertz mixers and direct intensity detectors, for far-infrared radiation. Our presentation also includes the novel approach to hot-electron quantum detection implemented in superconducting x-ray to optical photon counters.
TL;DR: In this article, the best modern analogues method was tested with 285 surface pollen spectra, and then applied to the 47,000 years pollen profile from Lake Mikata, Central Japan Both pollen taxa percentages and plant functional type affinity scores were used in the reconstruction of six climate variables.
TL;DR: In this article, the authors have modelled magma conduit flow incorporating viscosity variations caused by degassing and crystallization and modelled gas loss by permeable flow, with variations of permeability constrained by measurements.
Abstract: Growth of the andesitic lava dome during eruption of Soufriere Hills Volcano, Montserrat involved general increases in discharge rate and dome height with superposed fluctuations on timescales from hours to months. We have modelled magma conduit flow incorporating viscosity variations caused by degassing and crystallization. Gas loss is modelled by permeable flow, with variations of permeability constrained by measurements. Crystallization kinetics is modelled by an Avrami law. Observations and petrological studies constrain conduit diameter ( c . 30 m), magma chamber crystal content ( c . 60-65%), melt water content ( c . 5%), magma temperature ( c . 850°C) and chamber depth ( c . 5 km). Gas loss and rheological stiffening cause a maximum in magma overpressure (magma pressure minus lithostatic pressure) at depths of a few hundred metres below the dome. This maximum can explain ground deformation patterns, shallow seismicity, and short-lived Vulcanian explosions. Crystallization kinetics causes a strong feedback mechanism and multiple steady solutions for discharge rate. Small changes in chamber pressure, magma viscosity and conduit diameter strongly amplify discharge rate. Multiple solutions allow cyclic variation of discharge rate. Escalation of activity is attributed principally to influx of hot mafic magma into the chamber, resulting in chamber pressure increasing with time. Dome growth can be intrinsically unpredictable due to non-linear effects.
TL;DR: It is shown that desferrioxamine dose-dependents and time-dependently induces tolerance against focal cerebral ischemia in rats and mice, and against oxygen–glucose deprivation in purified cortical neurons.
Abstract: The widely prescribed drug desferrioxamine is a known activator of the hypoxia-inducible transcription factor 1 (HIF-1) and the subsequent transcription of erythropoietin. In the brain, HIF-1 is a master switch of the transcriptional response to hypoxia, whereas erythropoietin is a potent neuroprotectant. The authors show that desferrioxamine dose-dependently and time-dependently induces tolerance against focal cerebral ischemia in rats and mice, and against oxygen–glucose deprivation in purified cortical neurons. Desferrioxamine induced HIF-1 DNA binding and transcription of erythropoietin in vivo, the temporal kinetics of which were congruent with tolerance induction. Desferrioxamine is a promising drug for the induction of tolerance in humans when ischemia can be anticipated.
TL;DR: Evidence is provided that potyviral HCpro and hordeivirus γb proteins contribute to systemic viral infection, symptom severity, and RNA silencing suppression, and HCpro's ability to suppress the recovery of plants from viral infection emphasizes recovery as a manifestation of RNAsilencing.
Abstract: RNA silencing is a natural defense mechanism against genetic stress factors, including viruses. A mutant hordeivirus (Barley stripe mosaic virus [BSMV]) lacking the γb gene was confined to inoculated leaves in Nicotiana benthamiana, but systemic infection was observed in transgenic N. benthamiana expressing the potyviral silencing suppressor protein HCpro, suggesting that the γb protein may be a long-distance movement factor and have antisilencing activity. This was shown for γb proteins of both BSMV and Poa semilatent virus (PSLV), a related hordeivirus. Besides the functions in RNA silencing suppression, γb and HCpro had analogous effects on symptoms induced by the hordeiviruses. Severe BSMV-induced symptoms were correlated with high HCpro concentrations in the HCpro-transgenic plants, and substitution of the γb cistron of BSMV with that of PSLV led to greatly increased symptom severity and an altered pattern of viral gene expression. The efficient systemic infection with the chimera was followed by the development of dark green islands (localized recovery from infection) in leaves and exemption of new developing leaves from infection. Recovery and the accumulation of short RNAs diagnostic of RNA silencing in the recovered tissues in wild-type N. benthamiana were suppressed in HCpro-transgenic plants. These results provide evidence that potyviral HCpro and hordeivirus γb proteins contribute to systemic viral infection, symptom severity, and RNA silencing suppression. HCpro's ability to suppress the recovery of plants from viral infection emphasizes recovery as a manifestation of RNA silencing.
TL;DR: In this paper, experimental data on impurity states in narrow-gap lead telluride-based semiconductors are summarized and theoretical models describing the nontrivial properties of such states are presented.
Abstract: Experimental data on impurity states in narrow-gap lead telluride based semiconductors are summarized. Theoretical models describing the nontrivial properties of such states are presented. Applications to the design of highly sensitive far-infrared detectors are considered.
TL;DR: In this article, a reconstruction of Kerov's proof of Gaussian fluctuations around the limit shape of a curve is presented, largely based on the unpublished notes of the original proof.
Abstract: Consider random Young diagrams with fixed number n of boxes, distributed according to the Plancherel measure M n. That is, the weight M n(λ) of a diagram λ equals dim2 λ/n!, where dim λ denotes the dimension of the irreducible representation of the symmetric group indexed by λ. As n → ∞, the boundary of the (appropriately rescaled) random shape λ concentrates near a curve Ω (Logan-Shepp 1977, Vershik-Kerov 1977). In 1993, Kerov announced a remarkable theorem describing Gaussian fluctuations around the limit shape Ω. Here we propose a reconstruction of his proof. It is largely based on Kerov’s unpublished work notes, 1999
TL;DR: In this paper, the tetragonal (α) −orthorhombic (β) −monoclinic (γ) transitions in nanometric tungsten oxide samples were investigated.
TL;DR: The modern classification of small heat shock proteins (sHsp) is presented and peculiarities of their primary structure and the mechanism of formation of oligomeric complexes are described.
Abstract: The modern classification of small heat shock proteins (sHsp) is presented and peculiarities of their primary structure and the mechanism of formation of oligomeric complexes are described. Data on phosphorylation of sHsp by different protein kinases are presented and the effect of phosphorylation on oligomeric state and chaperone activity of sHsp is discussed. Intracellular location of sHsp under normal and stress conditions is described and it is emphasized that under certain condition sHsp interact with different elements of cytoskeleton. The literature concerning the effect of sHsp on polymerization of actin in vitro is analyzed. An attempt is made to compare effects of sHsp on polymerization of actin in vitro with the results obtained on living cells under normal conditions and after heat shock or hormone action. The literature concerning possible effects of sHsp on cell motility is also analyzed.
TL;DR: A survey of recent experimental results is given, presenting the growth of oxide epitaxial thin films in P−T−x conditions, which are far from those necessary for the existence of corresponding phases in a bulk state as mentioned in this paper.
Abstract: A survey of recent experimental results is given, presenting the growth of oxide epitaxial thin films in P−T−x conditions, which are far from those necessary for the existence of corresponding phases in a bulk state. The unstable in bulk BaCu3O4, NdMn7O12, RNiO3, and unusual polymorphous forms of BaRuO3, RMnO3, TiO2, and Mn3O4 are some examples. The stabilizing effect is observed only if epitaxial growth is induced. A rich variety of the effect observations are demonstrated to be of a thermodynamic origin, rather than of a kinetic one. Epitaxial stabilization is shown to be the result of the low energy of coherent interfaces formed due to epitaxy.
TL;DR: A systematic gene disruption approach was chosen to individually inactivate the majority of the cyanobacterial genes encoding peptidase subunits that are related to chloroplast enzymes, allowing classification of the peptidases that are required for cell viability or are involved in specific stress responses.
Abstract: A set of 62 genes that encode the entire peptidase complement of Synechocystis sp. PCC 6803 has been identified in the genome database of that cyanobacterium. Sequence comparisons with the Arabidopsis genome uncovered the presumably homologous chloroplast components inherited from their cyanobacterial ancestor. A systematic gene disruption approach was chosen to individually inactivate, by customary transformation strategies, the majority of the cyanobacterial genes encoding peptidase subunits that are related to chloroplast enzymes. This allowed classification of the peptidases that are required for cell viability or are involved in specific stress responses. The comparative analysis between Synechocystis and Arabidopsis chloroplast peptidases showed that: (1) homologous enzymes that arose by gene duplications in cyanobacteria are functionally diverse and frequently do not complement each other, (2) the chloroplast appears to house a number of distinct peptidase polypeptide chains of cyanobacterial origin (49) which is comparable with a cyanobacterial cell (62) and (3) the peptidase complement in plastids results from a combination of the loss of some cyanobacterial peptidases and the gain or diversification of subclasses of peptidases. This reorganization in the pattern of proteolytic enzymes may reflect distinct environmental and physiological changes between prokaryotic and organellar systems.
TL;DR: In this article, the authors discuss the possibility to introduce low noise damping produced by light into mechanical oscillator (one of the Fabry-Perot (FP) resonator mirrors is the mechanical resonator).
TL;DR: In this paper, the authors developed a generic model of magma discharge through a conduit from an open-system magma chamber with continuous replenishment, which takes account of the principal controls on flow, namely the replenishment rate, the chamber size, elastic deformation of the chamber walls, conduit resistance, and variations of the magma viscosity.