Nanjing University

About: Nanjing University is a education organization based out in Nanjing, China. It is known for research contribution in the topics: Catalysis & Population. The organization has 85961 authors who have published 105504 publications receiving 2289036 citations. The organization is also known as: NJU & Nanking University.

NF-kappaB and its regulation on the immune system.

Abstract: NF-kappaB is a transcription factor of eukaryote, whose family comprises five members in mammals and three in drosophila. Transcription factors of the NF-kappaB family are activated in response to signals that lead to cell growth, differentiation, apoptosis and other events. NF-kappaB takes part in expression of numerous cytokines and adhesion molecules which are critical elements involved in the regulation of immune responses. In this review, we focus on our current understanding of NF-kappaB signal pathway and its role in the innate and adaptive immune responses in which these transcription factors have a key regulatory function. Furthermore we review what is currently known about their effects associated with apoptosis.

Design and sensing applications of metal–organic framework composites

Abstract: As crystalline molecular materials, metal–organic frameworks (MOFs) have unique chemical and physical properties, such as ultrahigh porosity, tunable structure, and high thermal and chemical stability. These characteristics make MOFs suitable for use in gas storage, separation, catalysis, biomedical imaging and sensing. Precise chemical modifications can especially endow MOFs with specific functions and offer the possibility of designing a new generation of sensing devices. This article focuses on the design of functional MOFs and signal-transduction strategy, including optical, electrochemical, mechanical, and photoelectrochemical schemes, for analytical applications in detection of solvent molecules, metal ions, DNA, proteins, and other important biomolecules.

CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients

Abstract: Strategies that enhance the function of T cells are critical for immunotherapy. One negative regulator of T-cell activity is ligand PD-L1, which is expressed on dentritic cells (DCs) or some tumor cells, and functions through binding of programmed death-1 (PD-1) receptor on activated T cells. Here we described for the first time a non-viral mediated approach to reprogram primary human T cells by disruption of PD-1. We showed that the gene knockout of PD-1 by electroporation of plasmids encoding sgRNA and Cas9 was technically feasible. The disruption of inhibitory checkpoint gene PD-1 resulted in significant reduction of PD-1 expression but didn't affect the viability of primary human T cells during the prolonged in vitro culture. Cellular immune response of the gene modified T cells was characterized by up-regulated IFN-γ production and enhanced cytotoxicity. These results suggest that we have demonstrated an approach for efficient checkpoint inhibitor disruption in T cells, providing a new strategy for targeting checkpoint inhibitors, which could potentialy be useful to improve the efficacy of T-cell based adoptive therapies.