Nankai University

About: Nankai University is a education organization based out in Tianjin, China. It is known for research contribution in the topics: Catalysis & Adsorption. The organization has 42964 authors who have published 51866 publications receiving 1127896 citations. The organization is also known as: Nánkāi Dàxué.

Targeted Delivery of CRISPR/Cas9‐Mediated Cancer Gene Therapy via Liposome‐Templated Hydrogel Nanoparticles

Abstract: Due to its simplicity, versatility, and high efficiency, the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 technology has emerged as one of the most promising approaches for treatment of a variety of genetic diseases, including human cancers. However, further translation of CRISPR/Cas9 for cancer gene therapy requires development of safe approaches for efficient, highly specific delivery of both Cas9 and single guide RNA to tumors. Here, novel core–shell nanostructure, liposome-templated hydrogel nanoparticles (LHNPs) that are optimized for efficient codelivery of Cas9 protein and nucleic acids is reported. It is demonstrated that, when coupled with the minicircle DNA technology, LHNPs deliver CRISPR/Cas9 with efficiency greater than commercial agent Lipofectamine 2000 in cell culture and can be engineered for targeted inhibition of genes in tumors, including tumors the brain. When CRISPR/Cas9 targeting a model therapeutic gene, polo-like kinase 1 (PLK1), is delivered, LHNPs effectively inhibit tumor growth and improve tumor-bearing mouse survival. The results suggest LHNPs as versatile CRISPR/Cas9-delivery tool that can be adapted for experimentally studying the biology of cancer as well as for clinically translating cancer gene therapy.

GMS: Grid-Based Motion Statistics for Fast, Ultra-robust Feature Correspondence

Abstract: Feature matching aims at generating correspondences across images, which is widely used in many computer vision tasks. Although considerable progress has been made on feature descriptors and fast matching for initial correspondence hypotheses, selecting good ones from them is still challenging and critical to the overall performance. More importantly, existing methods often take a long computational time, limiting their use in real-time applications. This paper attempts to separate true correspondences from false ones at high speed. We term the proposed method (GMS) grid-based motion Statistics, which incorporates the smoothness constraint into a statistic framework for separation and uses a grid-based implementation for fast calculation. GMS is robust to various challenging image changes, involving in viewpoint, scale, and rotation. It is also fast, e.g., take only 1 or 2 ms in a single CPU thread, even when 50K correspondences are processed. This has important implications for real-time applications. What’s more, we show that incorporating GMS into the classic feature matching and epipolar geometry estimation pipeline can significantly boost the overall performance. Finally, we integrate GMS into the well-known ORB-SLAM system for monocular initialization, resulting in a significant improvement.

Artificial Virus Delivers CRISPR-Cas9 System for Genome Editing of Cells in Mice

Abstract: CRISPR-Cas9 has emerged as a versatile genome-editing platform. However, due to the large size of the commonly used CRISPR-Cas9 system, its effective delivery has been a challenge and limits its utility for basic research and therapeutic applications. Herein, a multifunctional nucleus-targeting “core-shell” artificial virus (RRPHC) was constructed for the delivery of CRISPR-Cas9 system. The artificial virus could efficiently load with the CRISPR-Cas9 system, accelerate the endosomal escape, and promote the penetration into the nucleus without additional nuclear-localization signal, thus enabling targeted gene disruption. Notably, the artificial virus is more efficient than SuperFect, Lipofectamine 2000, and Lipofectamine 3000. When loaded with a CRISPR-Cas9 plasmid, it induced higher targeted gene disruption efficacy than that of Lipofectamine 3000. Furthermore, the artificial virus effectively targets the ovarian cancer via dual-receptor-mediated endocytosis and had minimum side effects. When loaded with...