Showing papers by "National Autonomous University of Mexico published in 2002"

New catalogue of optically visible open clusters and candidates

Abstract: We have compiled a new catalogue of open clusters in the Galaxy which updates the previous cat- alogues of Lyng a (1987) and of Mermilliod (1995) (included in the WEBDA database). New objects and new data, in particular, data on kinematics (proper motions) that were not present in the old catalogues, have been included. Virtually all the clusters (1537) presently known were included, which represents an increment of about 347 objects relative to the Lyng a (1987) catalogue. The catalogue is presented in a single table con- taining all the important data, which makes it easy to use. The catalogue can be accessed on line either at http://www.astro.iag.usp.br/~wilton/ or as an electronic table which will be made available at the CDS.

Future projections for Mexican faunas under global climate change scenarios

Abstract: Global climates are changing rapidly, with unexpected consequences. Because elements of biodiversity respond intimately to climate as an important driving force of distributional limitation, distributional shifts and biodiversity losses are expected. Nevertheless, in spite of modelling efforts focused on single species or entire ecosystems, a few preliminary surveys of fauna-wide effects, and evidence of climate change-mediated shifts in several species, the likely effects of climate change on species' distributions remain little known, and fauna-wide or community-level effects are almost completely unexplored. Here, using a genetic algorithm and museum specimen occurrence data, we develop ecological niche models for 1,870 species occurring in Mexico and project them onto two climate surfaces modelled for 2055. Although extinctions and drastic range reductions are predicted to be relatively few, species turnover in some local communities is predicted to be high (>40% of species), suggesting that severe ecological perturbations may result.

Formation and Growth of Au Nanoparticles inside Live Alfalfa Plants

Abstract: In modern nanotechnology one of the most exciting areas is the interaction between inorganic quantum dots and biological structures. For instance gold clusters surrounded by a shell of organic ligands covalently attach to proteins or other biological substances and can be used for labeling in structural biology. In the present report we show the possibility of using live plants for the fabrication of nanoparticles. Alfalfa plants were grown in an AuCl4 rich environment. The absorption of Au metal by the plants was confirmed by X-ray absorption studies (XAS), and transmission electron microscopy (TEM). Atomic resolution analysis confirmed the nucleation and growth of Au nanoparticles inside the plant and that the Au nanoparticles are in a crystalline state. Images also showed defects such as twins in the crystal structure, and in some cases icosahedral nanoparticles were found. X-ray EDS studies corroborated that the nanoparticles are pure gold. This is the first report on the formation of gold nanoparticl...

Mammal Population Losses and the Extinction Crisis

Abstract: The disappearance of populations is a prelude to species extinction. No geographically explicit estimates have been made of current population losses of major indicator taxa. Here we compare historic and present distributions of 173 declining mammal species from six continents. These species have collectively lost over 50% of their historic range area, mostly where human activities are intensive. This implies a serious loss of ecosystem services and goods. It also signals a substantial threat to species diversity.

A Unique Short-Chain Dehydrogenase/Reductase in Arabidopsis Glucose Signaling and Abscisic Acid Biosynthesis and Functions

Abstract: Glc has hormone-like functions and controls many vital processes through mostly unknown mechanisms in plants. We report here on the molecular cloning of GLUCOSE INSENSITIVE1 (GIN1) and ABSCISIC ACID DEFICIENT2 (ABA2) which encodes a unique Arabidopsis short-chain dehydrogenase/reductase (SDR1) that functions as a molecular link between nutrient signaling and plant hormone biosynthesis. SDR1 is related to SDR superfamily members involved in retinoid and steroid hormone biosynthesis in mammals and sex determination in maize. Glc antagonizes ethylene signaling by activating ABA2/GIN1 and other abscisic acid (ABA) biosynthesis and signaling genes, which requires Glc and ABA synergistically. Analyses of aba2/gin1 null mutants define dual functions of endogenous ABA in inhibiting the postgermination developmental switch modulated by distinct Glc and osmotic signals and in promoting organ and body size and fertility in the absence of severe stress. SDR1 is sufficient for the multistep conversion of plastid- and carotenoid-derived xanthoxin to abscisic aldehyde in the cytosol. The surprisingly restricted spatial and temporal expression of SDR1 suggests the dynamic mobilization of ABA precursors and/or ABA.

Evidence for a Developing Gap in a 10 Myr Old Protoplanetary Disk

Abstract: We have developed a physically self-consistent model of the disk around the nearby 10 Myr old star TW Hya that matches the observed spectral energy distribution and 7 mm images of the disk. The model requires both significant dust-size evolution and a partially evacuated inner disk region, as predicted by theories of planet formation. The outer disk, which extends to at least 140 AU in radius, is very optically thick at infrared wavelengths and quite massive (~0.06 M☉) for the relatively advanced age of this T Tauri star. This implies long viscous and dust evolution timescales, although dust must have grown to sizes of the order of ~1 cm to explain the submillimeter and millimeter spectral slopes. In contrast, the negligible near-infrared excess emission of this system requires that the disk be optically thin inside 4 AU. This inner region cannot be completely evacuated; we need ~0.5 lunar mass of ~1 μm particles remaining to produce the observed 10 μm silicate emission. Our model requires a distinct transition in disk properties at ~4 AU separating the inner and outer disks. The inner edge of the optically thick outer disk must be heated almost frontally by the star to account for the excess flux at mid-infrared wavelengths. We speculate that this truncation of the outer disk may be the signpost of a developing gap due to the effects of a growing protoplanet; the gap is still presumably evolving because material still resides in it, as indicated by the silicate emission, the molecular hydrogen emission, and the continued accretion onto the central star (albeit at a much lower rate than typical of younger T Tauri stars). Thus, TW Hya may become the Rosetta stone for our understanding of the evolution and dissipation of protoplanetary disks.

The EcoCyc Database

Abstract: EcoCyc is an organism-specific pathway/genome database that describes the metabolic and signal-transduction pathways of Escherichia coli, its enzymes, its transport proteins and its mechanisms of transcriptional control of gene expression. EcoCyc is queried using the Pathway Tools graphical user interface, which provides a wide variety of query operations and visualization tools. EcoCyc is available at http://ecocyc.org/.

Plant Lipoxygenases. Physiological and Molecular Features

Abstract: Lipoxygenases (LOXs; EC[1.13.11.12][1]) are nonheme iron-containing dioxygenases widely distributed in plants and animals. LOX catalyzes the addition of molecular oxygen to polyunsaturated fatty acids containing a ( Z , Z )-1,4-pentadiene system to produce an unsaturated fatty acid hydroperoxide.

Limits to the use of threatened species lists

Abstract: Threatened species lists are designed primarily to provide an easily understood qualitative estimate of risk of extinction. Although these estimates of risk can be accurate, the lists have inevitably become linked to several decision-making processes. There are four ways in which such lists are commonly used: to set priorities for resource allocation for species recovery; to inform reserve system design; to constrain development and exploitation; and to report on the state of the environment. The lists were not designed for any one of these purposes, and consequently perform some of them poorly. We discuss why, if and how they should be used to achieve these purposes.

Current Consensus Guidelines for Treatment of Neurocysticercosis

Abstract: Taenia solium neurocysticercosis is a common cause of epileptic seizures and other neurological morbidity in most developing countries. It is also an increasingly common diagnosis in industrialized countries because of immigration from areas where it is endemic. Its clinical manifestations are highly variable and depend on the number, stage, and size of the lesions and the host's immune response. In part due to this variability, major discrepancies exist in the treatment of neurocysticercosis. A panel of experts in taeniasis/cysticercosis discussed the evidence on treatment of neurocysticercosis for each clinical presentation, and we present the panel's consensus and areas of disagreement. Overall, four general recommendations were made: (i) individualize therapeutic decisions, including whether to use antiparasitic drugs, based on the number, location, and viability of the parasites within the nervous system; (ii) actively manage growing cysticerci either with antiparasitic drugs or surgical excision; (iii) prioritize the management of intracranial hypertension secondary to neurocysticercosis before considering any other form of therapy; and (iv) manage seizures as done for seizures due to other causes of secondary seizures (remote symptomatic seizures) because they are due to an organic focus that has been present for a long time.

Brain Activity Underlying Encoding and Retrieval of Source Memory

Abstract: Neural activity elicited during the encoding and retrieval of source information was investigated with event-related functional magnetic resonance imaging (efMRI). During encoding, 17 subjects performed a natural/artificial judgement on pictures of common objects which were presented randomly in one of the four quadrants of the display. At retrieval, old pictures were mixed with new ones and subjects judged whether each picture was new or old and, if old, indicated in which quadrant it was presented at encoding. During encoding, study items that were later recognized and assigned a correct source judgement elicited greater activity than recognized items given incorrect judgements in a variety of regions, including right lateral occipital and left prefrontal cortex. At retrieval, regions showing greater activity for recognized items given correct versus incorrect source judgements included the right hippocampal formation and the left prefrontal cortex. These findings indicate a role for these regions in the encoding and retrieval of episodic information beyond that required for simple item recognition.

Ancient DNA Evidence for Old World Origin of New World Dogs

Abstract: Mitochondrial DNA sequences isolated from ancient dog remains from Latin America and Alaska showed that native American dogs originated from multiple Old World lineages of dogs that accompanied late Pleistocene humans across the Bering Strait. One clade of dog sequences was unique to the New World, which is consistent with a period of geographic isolation. This unique clade was absent from a large sample of modern dogs, which implies that European colonists systematically discouraged the breeding of native American dogs.

Planning to Save a Species: the Jaguar as a Model

Abstract: International conservation planning at the end of the twentieth century is dominated by coarse-filter, supra-organismal approaches to conservation that may be insufficient to conserve certain species such as the jag- uar ( Panthera onca ). If we are to retain broadly distributed species into the next century, we need to plan explic- itly for their survival across their entire geographic range and through political boundaries while recognizing the variety of ecological roles the species plays in different habitats. In March 1999 the Wildlife Conservation Society sponsored a priority-setting and planning exercise for the jaguar across its range, from northern Mexico to north- ern Argentina. Field scientists from 18 countries reached consensus on four types of information: (1) the spatial extent of their jaguar knowledge, (2) the known, currently occupied range of jaguars, (3) areas with substantial jaguar populations, adequate habitat, and a stable and diverse prey base, and (4) point localities where jaguars have been observed during the last 10 years. During the exercise, these experts also conducted a range-wide as- sessment of the long-term survival prospects of the jaguar and developed an algorithm for prioritizing jaguar conservation units occurring in major habitat types. From this work, we learned that the known, occupied range of the jaguar has contracted to approximately 46% of estimates of its 1900 range. Jaguar status and distribution is unknown in another 12% of the jaguar's former range, including large areas in Mexico, Colombia, and Brazil. But over 70% of the area where jaguars are thought to still occur was rated as having a high probability of sup- porting their long-term survival. Fifty-one jaguar conservation units representing 30 different jaguar geographic regions were prioritized as the basis for a comprehensive jaguar conservation program.

Neuronal correlates of decision-making in secondary somatosensory cortex

Abstract: The ability to discriminate between two sequential stimuli requires evaluation of current sensory information in reference to stored information Where and how does this evaluation occur? We trained monkeys to compare two mechanical vibrations applied sequentially to the fingertips and to report which of the two had the higher frequency We recorded single neurons in secondary somatosensory cortex (S2) while the monkeys performed the task During the first stimulus period, the firing rate of S2 neurons encoded the stimulus frequency During the second stimulus period, however, some S2 neurons did not merely encode the stimulus frequency The responses of these neurons were a function of both the remembered (first) and current (second) stimulus Moreover, a few hundred milliseconds after the presentation of the second stimulus, these responses were correlated with the monkey's decision This suggests that some S2 neurons may combine past and present sensory information for decision-making

Connectivity based k-hop clustering in wireless networks

Abstract: In this paper we describe several new clustering algorithms for nodes in a mobile ad hoc network. We propose to combine two known approaches into a single clustering algorithm which considers connectivity as a primary criterion and lower ID as secondary criterion for selecting cluster heads. The goal is to minimize the number of clusters, which results in dominating sets of smaller sizes (this is important for applications in broadcasting and Bluetooth formation). We also describe algorithms for modifying cluster structure in the presence of topological changes. Next, we generalize the cluster definition so that a cluster contains all nodes that are at a distance of at most k hops from the cluster head. The efficiency of four clustering algorithms (k-lowestID and k-CONID, k=1 and k=2) is tested by measuring the average number of created clusters, the number of border nodes, and the cluster size in random unit graphs. The most interesting experimental result is stability of the ratio of the sum of CHs and border nodes in the set. It was constantly 60-70% for 1-lowestID and 46-56% for 1-ConID, for any value of n (number of nodes) and d (average node degree). Similar conclusions and similar number were obtained for k=2. We also proposed a unified framework for most existing and new clustering algorithms where a properly defined weight at each node is the only difference in the algorithm. Finally, we propose a framework for generating random unit graphs with obstacles.

Use of Tenebrio molitor (Coleoptera: Tenebrionidae) to Recycle Organic Wastes and as Feed for Broiler Chickens

Abstract: Several dried waste materials from different origins were used as a substrate to grow Tenebrio molitor L. Nutrient/amino acid values differed depending on both larval size/weight and substrate. These larvae were experimentally used as a broiler feedstuff. Seven-day-old chicks of a commercially available strain with an average weight of 126 g were randomly distributed into nine six-broiler groups. Three levels of Tenebrio molitor larvae (0, 5, and 10% dry weight) were used in a 19% protein content sorghum-soybean meal basal diet, to evaluate feed intake, weight gain, and feed efficiency. Results after 15 d showed no significant differences among treatments. These data indicate that Tenebrio molitor has the potential to be used as protein source for raising broilers.

Loop quantum gravity and light propagation

Abstract: Within loop quantum gravity we construct a coarse-grained approximation for the Einstein-Maxwell theory that yields effective Maxwell equations in flat spacetime comprising Planck scale corrections. The corresponding Hamiltonian is defined as the expectation value of the electromagnetic term in the Einstein-Maxwell Hamiltonian constraint, regularized in the manner of Thiemann, with respect to a would-be semiclassical state. The resulting energy dispersion relations entail Planck scale corrections to those in flat spacetime. Both the helicity dependent contribution of Gambini and Pullin and, for a value of a parameter of our approximation, that of Ellis and co-workers are recovered. The electric-magnetic asymmetry in the regularization procedure yields nonlinearities only in the magnetic sector which are briefly discussed. Observations of cosmological gamma ray bursts might eventually lead to the needed accuracy to study some of these quantum gravity effects.

Regulatory Circuitry of the CsrA/CsrB and BarA/UvrY Systems of Escherichia coli

Abstract: The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globular ribonucleoprotein complex. CsrA indirectly activates csrB transcription, in an apparent autoregulatory mechanism. In the present study, we elucidate the intermediate regulatory circuitry of this system. Mutations affecting the BarA/UvrY two-component signal transduction system decreased csrB transcription but did not affect csrA′-′lacZ expression. The uvrY defect was severalfold more severe than that of barA. Both csrA and uvrY were required for optimal barA expression. The latter observation suggests an autoregulatory loop for UvrY. Ectopic expression of uvrY suppressed the csrB-lacZ expression defects caused by uvrY, csrA, or barA mutations; csrA suppressed csrA or barA defects; and barA complemented only the barA mutation. Purified UvrY protein stimulated csrB-lacZ expression approximately sixfold in S-30 transcription-translation reactions, revealing a direct effect of UvrY on csrB transcription. Disruption of sdiA, which encodes a LuxR homologue, decreased the expression of uvrY′-′lacZ and csrB-lacZ fusions but did not affect csrA′-′lacZ. The BarA/UvrY system activated biofilm formation. Ectopic expression of uvrY stimulated biofilm formation by a csrB-null mutant, indicative of a CsrB-independent role for UvrY in biofilm development. Collectively, these results demonstrate that uvrY resides downstream from csrA in a signaling pathway for csrB and that CsrA stimulates UvrY-dependent activation of csrB expression by BarA-dependent and -independent mechanisms.

Astrophysical and astrochemical insights into the origin of life

Abstract: Stellar nucleosynthesis of heavy elements such as carbon allowed the formation of organic molecules in space, which appear to be widespread in our Galaxy. The physical and chemical conditions—including density, temperature, ultraviolet (UV) radiation and energetic particles—determine reaction pathways and the complexity of organic molecules in different space environments. Dense interstellar clouds are the birth sites of stars of all masses and their planetary systems. During the protostellar collapse, interstellar organic molecules in gaseous and solid phases are integrated into protostellar disks from which planets and smaller solar

Roles of prolactin and related members of the prolactin/growth hormone/placental lactogen family in angiogenesis

Abstract: Prolactin, growth hormone and placental lactogen are members of a family of polypeptide hormones which share structural similarities and biological activities. Numerous functions have been attributed to these hormones, among which stand out their recently discovered effects on angiogenesis, the process by which new blood vessels are formed from the pre-existing microvasculature. Prolactin, growth hormone and placental lactogen, along with two non-classical members of the family, proliferin and proliferin-related protein, can act both as circulating hormones and as paracrine/autocrine factors to either stimulate or inhibit various stages of the formation and remodeling of new blood vessels, including endothelial cell proliferation, migration, protease production and apoptosis. Such opposing actions can reside in similar but independent molecules, as is the case of proliferin and proliferin-related protein, which stimulate and inhibit angiogenesis respectively. The potential to exert opposing effects on angiogenesis can also reside within the same molecule as the parent protein can promote angiogenesis (i.e. prolactin, growth hormone and placental lactogen), but after proteolytic processing the resulting peptide fragment acquires anti-angiogenic properties (i.e. 16 kDa prolactin, 16 kDa growth hormone and 16 kDa placental lactogen). The unique properties of the peptide fragments versus the full-length molecules, the regulation of the protease responsible for specific protein cleavage, the selective expression of specific receptors and their associated signal transduction pathways are issues that are being investigated to further establish the precise contribution of these hormones to angiogenesis under both physiological and pathological situations. In this review article, we summarize the known and speculative issues underlying the effects of the prolactin, growth hormone and placental lactogen family of proteins on angiogenesis, and address important remaining enigmas in this field of research.

An accurate and efficient Bayesian method for automatic segmentation of brain MRI

Abstract: Automatic three-dimensional (3-D) segmentation of the brain from magnetic resonance (MR) scans is a challenging problem that has received an enormous amount of attention lately Of the techniques reported in the literature, very few are fully automatic In this paper, we present an efficient and accurate, fully automatic 3-D segmentation procedure for brain MR scans It has several salient features; namely, the following 1) Instead of a single multiplicative bias field that affects all tissue intensities, separate parametric smooth models are used for the intensity of each class 2) A brain atlas is used in conjunction with a robust registration procedure to find a nonrigid transformation that maps the standard brain to the specimen to be segmented This transformation is then used to: segment the brain from nonbrain tissue; compute prior probabilities for each class at each voxel location and find an appropriate automatic initialization 3) Finally, a novel algorithm is presented which is a variant of the expectation-maximization procedure, that incorporates a fast and accurate way to find optimal segmentations, given the intensity models along with the spatial coherence assumption Experimental results with both synthetic and real data are included, as well as comparisons of the performance of our algorithm with that of other published methods

Effects of dry tropical forest fragmentation on the reproductive success and genetic structure of the tree Samanea saman.

Abstract: Tropical trees are particularly vulnerable to forest fragmentation because of their low densities, self-incompatibilty system, and high rates of outbreeding. Forest fragmentation is likely to decrease gene flow, increase endogamy, and eventually produce a high differentiation among remnant populations. Our objectives were to evaluate the effect of forest fragmentation on the reproductive success, progeny vigor, and genetic variation of the tropical dry-forest tree Samanea saman. We conducted our study in the dry forest of Costa Rica and compared two tree conditions to evaluate forest fragmentation: (1) isolated, individual trees more than 500 m from the nearest conspecific and surrounded by agricultural fields, pastures, or small remnant forest patches and (2) trees in continuous populations of 10 or more individuals per hectare and surrounded by undisturbed forest. We compared the probability of natural pollination, seed production, genetic variation, and progeny vigor of trees in isolation and in continuous populations. The probability of flowers receiving more than 23 pollen tubes at the base of styles (the flowers more likely to produce a mature fruit) was significantly greater for trees in continuous populations (2.1%) than for trees in isolation (0.4%). However, flowers from the two tree conditions received similar pollen loads on their stigmas. Fruits of trees from continuous populations produced similar numbers of seeds as isolated trees did and had a similar probability of seed abortion. The probability of seed predation by bruchid beetles was significantly greater in continuous populations (30%) than in isolated trees (20%). The number of undamaged seeds ( potentially viable) was similar for both tree conditions. A genetic analysis of progeny showed that levels of genetic diversity in trees in isolation and in continuous populations were comparable. The effective self-fertilization rate and inbreeding coefficient of the progeny were slightly higher for isolated trees than for trees in continuous populations. In addition, there was more genetic similarity in the progeny of isolated trees, within and between fruits. Seeds produced by different fruits within a tree were more likely to be related in an isolated tree than in a tree from continuous populations. Seeds produced by trees from continuous populations were more likely to germinate and to produce greater leaf area and biomass as seedlings than progeny from isolated trees. We concluded that the fragmentation of tropical dry forests affects the genetic variation and vigor of S. saman progeny. Isolated trees showed high reproductive capacity, however, in spite of their habitat condition. Resumen: Los arboles tropicales son particularmente vulnerables a la fragmentacion de bosques debido a su baja densidad, su sistema de auto-incompatibilidad y altas tasas de exogamia. La fragmentacion del bosque posiblemente decrece el flujo de genes, incrementa la endogamia y eventualmente produce una alta diferenciacion entre las poblaciones remanentes. Nuestros objetivos fueron evaluar el efecto de la fragmentacion del bosque sobre el exito reproductivo, el vigor de la progenie y la variacion genetica del arbol de bosque tropical seco Samanea saman. Realizamos nuestro estudio en el bosque tropical seco de Costa Rica y comparamos dos condiciones de los arboles para evaluar la fragmentacion del bosque: (1) individuos aislados mas de 500 m del individuo conespecifico mas cercano y rodeados por campos agricolas, ganaderos o pequenos fragmentos de bosque y (2) arboles en poblaciones continuas de 10 o mas individuos por hectarea y rodeados de bosque no perturbado. Comparamos la probabilidad de la polinizacion natural, produccion de semillas, variacion genetica y vigor de la progenie en arboles aislados y en poblaciones continuas. La probabilidad de que las flores reciban mas de 23 tubos de polen en la base de los pistilos ( las flores con mayor probabilidad de producir un fruto maduro) fue significativamente mayor para arboles en poblaciones continuas (2.1%) que en arboles aislados (0.4%). Sin embargo, flores en arboles en las dos condiciones recibieron similares cargas de polen en sus estigmas. Los frutos de arboles de poblaciones continuas produjeron similares numeros de semillas y tuvieron similar probabilidad de abortar que los de arboles aislados. La probabilidad de depredacion de semillas por escarabajos bruquidos fue significativamente mayor en las poblaciones continuas (30%) que en arboles aislados (20%). El numero de semillas sin dano ( potencialmente viables) fue similar en arboles en las dos condiciones. El analisis genetico de la progenie mostro que los niveles de diversidad genetica de arboles aislados y en poblaciones continuas fueron comparables. La tasa de autofecundacion efectiva y el coeficiente de endogamia de la progenie fue ligeramente mayor en arboles aislados que en los de poblaciones continuas. Adicionalmente, hubo mayor similitud genetica en la progenie de arboles aislados, dentro y entre frutos. Semillas producidas por distintos frutos del mismo arbol tuvieron mas probabilidad de relacion que los de poblaciones continuas. Semillas producidas por arboles en poblaciones continuas tuvieron mayor posibilidad de germinar y producir mayor area foliar y biomasa que las plantulas de la progenie de arboles aislados. Concluimos que la fragmentacion de bosques tropicales secos afecta la variacion genetica y el vigor de la progenie de S. saman. Sin embargo, los arboles aislados tuvieron una alta capacidad reproductiva a pesar de la condicion de su habitat.

Is metronidazole carcinogenic

Abstract: Metronidazole (MTZ, 1-[2-hydroxyethyl]-2-methyl-5-nitroimidazole), an antiparasitic and antibacterial compound, is one of the world's most used drugs. MTZ is potentially carcinogenic to humans due to the following facts: it is a proven mutagen in bacterial systems, it is genotoxic to human cells and also, it is carcinogenic to animals. However, due to inadequate epidemiological evidence, it is not considered as a risk factor for cancer in humans. As it will be discussed here, the existing population studies are deficient since they have not included sufficient sample size, the follow-up time has not been long enough, and the individual sensitivity to the drug might have been acting as a confounding factor. Due to the increasing use of this drug, more and improved studies are needed to elucidate its mechanism of genotoxicity and its carcinogenic potential.