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Institution

National Chemical Laboratory

FacilityPune, Maharashtra, India
About: National Chemical Laboratory is a facility organization based out in Pune, Maharashtra, India. It is known for research contribution in the topics: Catalysis & Enantioselective synthesis. The organization has 8891 authors who have published 14837 publications receiving 387600 citations.


Papers
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Journal ArticleDOI
TL;DR: Direct regeneration of shoot buds was observed in MS basal medium supplemented with various concentrations of either benzyladenine (BA) or thidiazouron (TDZ) depending on the explant.
Abstract: In vitro studies were initiated with Withania somnifera (L.) Dun. for rapid micropropagation of selected chemotypes using nodes, internodes, hypocotyls and embryo explants. Direct regeneration of shoot buds was observed in MS basal medium supplemented with various concentrations of either benzyladenine (BA) or thidiazouron (TDZ) depending on the explant. Nodal explants formed multiple shoots both from pre-existing and de novo buds on Murashige and Skoog's medium (MS) containing 0.1–5.0 mg l−1 BA and a ring of de novo shoot buds on MS medium containing 0.2 and 0.3 mg l−1 TDZ. Internodal explants formed shoot buds on MS with 1.0 and 5.0 mg l−1 BA while the hypocotyl explants gave rise to multiple shoots only on MS with 0.5 mg l−1 BA. Isolated embryos gave rise to many shoot buds on MS with 0.2 and 0.3 mg l−1 TDZ. The shoot buds elongated and rooted either on MS medium with 0.01 mg l−1 BA or on half strength MS medium lacking growth regulators, which depended upon the growth regulator used in the shoot bud induction medium. Except for the embryo-derived plantlets, all other plantlets could be acclimatized with 100% success.

93 citations

Journal ArticleDOI
TL;DR: A PCR-based Seedling Sex Diagnostic Assay (SSDA) specially designed for early sexing of papaya seedlings is reported here and could be easily applied to molecular analysis of any agriculturally important trait for which specific DNA probes could be identified and hence opens new avenues of research in the field of genetic diagnostics of plants.
Abstract: Female plants of several dioecious angiosperms are commercially valued for production of fruits or seeds, viz. papaya, nutmeg, pistachio, kiwi fruit and jojoba. To make the cultivation profitable it is necessary to grow more female than male plants. To discriminate between male and female plants, sex-specific molecular markers have been identified in a few dioecious species such as Silene and pistachio. However, accurate and convenient sex diagnostic methods for early sexing of seedlings are not available to date. For the first time, we report here a PCR-based Seedling Sex Diagnostic Assay (SSDA) specially designed for early sexing of papaya seedlings. We have developed a male-specific SCAR marker in papaya by cloning a male-specific RAPD (831 bp) fragment and designing longer primers. The potential of this SCAR marker is further exploited to develop a simplified and highly accurate sex diagnostic assay by (1) including an internal PCR control, (2) following a single-step DNA extraction procedure and (3) optimising the PCR conditions to simultaneously amplify male-specific and control bands from the crude leaf extract. This diagnostic approach would be of great commercial significance to papaya growers as well as to seed companies and plant nurseries for early identification of female seedlings of dioecious species. In principle, this experimental design could be easily applied to molecular analysis of any agriculturally important trait for which specific DNA probes could be identified and hence opens new avenues of research in the field of genetic diagnostics of plants.

92 citations

Journal ArticleDOI
08 May 2009-Langmuir
TL;DR: In addition to providing an elegant means of controlling the ionic cluster size, the strategic approach of using CNT both as an anchoring backbone for -SO(3)H groups to enrich proton conductivity and as a blending agent to improve the mechanical characteristics of the Nafion phase might be helpful in alleviating many critical problems associated with the use of commercial Nafions.
Abstract: The application of sulfonic acid-functionalized multiwalled (s-MWNT) carbon nanotubes to manipulate the hydrophilic domain size of Nafion membranes is explored here as an option for tuning the proton conductivity of polymer electrolyte membranes for hydrogen−oxygen fuel cells. The electrochemical impedance experiments provide preliminary evidence of increased proton conductivity, while small-angle X-ray scattering measurements line out enhanced ionic cluster domain size in these composite membranes as the central reason for higher conductivity (70 A for the optimum composite membrane vs 50 A for Nafion 115) values. Scanning electrochemical microscopy indicates synergistic interaction between the sulfonic acid functional groups present in the Nafion membrane and those on the nanotube surface. More interestingly, the nanotube-tailored Nafion membranes ameliorate the performance of fuel cells as confirmed by measurements at a single-cell level, which reveal a maximum power density of 380 mW cm−2, higher than...

92 citations

Journal ArticleDOI
TL;DR: This hybrid ''MOF@CNF'' displayed improved thermal stability as well as gas adsorption compared to the individual counterparts.

92 citations


Authors

Showing all 8913 results

NameH-indexPapersCitations
Ashok Kumar1515654164086
Rajesh Kumar1494439140830
Tak W. Mak14880794871
John T. O'Brien12181963242
Clive Ballard11773661663
Yoshinori Tokura11785870258
John S. Mattick11636764315
Michael Dean10741963335
Ian G. McKeith10746851954
David J. Burn10044639120
Anil Kumar99212464825
Vikas Kumar8985939185
Detlef W. Bahnemann8851748826
Gautam R. Desiraju8845845301
Praveen Kumar88133935718
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Performance
Metrics
No. of papers from the Institution in previous years
YearPapers
20236
202238
2021482
2020454
2019471
2018498