National Cheng Kung University

About: National Cheng Kung University is a education organization based out in Tainan City, Taiwan. It is known for research contribution in the topics: Population & Thin film. The organization has 49723 authors who have published 69799 publications receiving 1437420 citations. The organization is also known as: NCKU.

Enhanced Production of Surfactin from Bacillussubtilis by Addition of Solid Carriers

Abstract: Addition of a small quantity of solid porous carriers (e.g., activated carbon or expanded clay) into fermentation broth significantly increased surfactin production with Bacillus subtilis ATCC 21332. Culture medium containing 25 g L -1 of activated carbon gave an optimal surfactin yield of 3600 mg L -1 , which was approximately 36-fold higher than that obtained from carrier-free liquid culture. The marked increase in surfactin production was primarily attributed to stimulation of cell growth due to the presence of activated carbon carriers. Concentration of limiting carbon substrate (glucose) is also an important factor affecting the production of surfactin, as an initial glucose concentration of 40 g L -1 resulted in optimal surfactin production. An appropriate agitation rate also benefited surfactin production, as the best yield appeared at an agitation rate of 200 rpm. Surfactin was purified from fermentation broth via a series of acidic precipitation and solvent extraction. The resulting product was nearly 90% pure with a recovery efficiency of ca. 72%. The purified surfactin reduced the surface tension of water from 72 to 27 mN m -1 with a critical micelle concentration of ca. 10 mg L -1 . The surfactin product also attained an emulsion index of 70% for kerosene and diesel at a low concentration of 100 and 600 mg L -1 , respectively.

Cytotoxic and antimalarial β-carboline alkaloids from the roots of Eurycoma longifolia

Abstract: Three new [n-pentyl β-carboline-1-propionate (1), 5-hydroxymethyl-9-methoxycanthin-6-one (2), and 1-hydroxy-9-methoxycanthin-6-one (3)] and 19 known β-carboline alkaloids were isolated from the roots of Eurycoma longifolia. The new structures were determined by comprehensive analyses of their 1D and 2D NMR and mass spectral data and by chemical transformation. These compounds were screened for in vitro cytotoxic and antimalarial activities, and 9-methoxycanthin-6-one (4) and canthin-6-one (5) demonstrated significant cytotoxicity against human lung cancer (A-549) and human breast cancer (MCF-7) cell lines.

Noncontact Measurement of Complex Permittivity and Thickness by Using Planar Resonators

Abstract: This paper presents a novel noncontact measurement technique that entails using a single-compound triple complementary split-ring resonator (SC-TCSRR) to determine the complex permittivity and thickness of a material under test (MUT). The proposed technique overcomes the problem engendered by the existence of air gaps between the sensor ground plane and the MUT. In the proposed approach, a derived governing equation of the resonance frequencies is used to estimate the thickness and complex permittivity of the MUT by calculating the resonant frequency $(f_{{ r}})$ and magnitude response in a single-step noncontact measurement process. This study theoretically analyzed and experimentally verified a simple and low-cost SC-TCSRR measurement method for assessing materials in a noncontact method. For a 0.2-mm air gap, the experiments yielded average measurement errors of 4.32% and 5.05% for the thickness and permittivity, respectively. The proposed SC-TCSRR technique provides excellent solutions for reducing the effect of air-gap conditions on permittivity, thickness, and loss tangent in noncontact measurements.

Ethnic Differences and Functional Analysis of MET Mutations in Lung Cancer

Abstract: Purpose: African Americans have higher incidence and poorer response to lung cancer treatment compared with Caucasians. However, the underlying molecular mechanisms for the significant ethnic difference are not known. The present study examines the ethnic differences in the type and frequency of MET proto-oncogene (MET) mutation in lung cancer and correlated them with other frequently mutated genes such as epidermal growth factor receptor (EGFR), KRAS2, and TP53. Experimental Design: Using tumor tissue genomic DNA from 141 Asian, 76 Caucasian, and 66 African American lung cancer patients, exons coding for MET and EGFR were PCR amplified, and mutations were detected by sequencing. Mutation carriers were further screened for KRAS2 and TP53 mutations. Functional implications of important MET mutations were explored by molecular modeling and hepatocyte growth factor binding studies. Results: Unlike the frequently encountered somatic mutations in EGFR, MET mutations in lung tumors were germline. MET-N375S, the most frequent mutation of MET, occurred in 13% of East Asians compared with none in African Americans. The frequency of MET mutations was highest among male smokers and squamous cell carcinoma. The MET-N375S mutation seems to confer resistance to MET inhibition based on hepatocyte growth factor ligand binding, molecular modeling, and apoptotic susceptibility to MET inhibitor studies. Conclusions: MET in lung cancer tissues contained nonsynonymous mutations in the semaphorin and juxtamembrane domains but not in the tyrosine kinase domain. All the MET mutations were germline. East Asians, African-Americans, and Caucasians had different MET genotypes and haplotypes. MET mutations in the semaphorin domain affected ligand binding. (Clin Cancer Res 2009;15(18):5714–23)